| Naslov: | SNAP-25b-deficiency increases insulin secretion and changes spatiotemporal profile of $Ca^{2+}$ oscillations in $\beta$ cell networks |
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| Avtorji: | Daraio, Teresa (Avtor)
Križančić Bombek, Lidija (Avtor)
Gosak, Marko (Avtor)
Valladolid-Acebes, Ismael (Avtor)
Skelin, Maša (Avtor)
Refai, Essam (Avtor)
Berggren, Per-Olof (Avtor)
Brismar, Kerstin (Avtor)
Rupnik, Marjan (Avtor)
Bark, Christina (Avtor) |
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| Datoteke: |  Scientific_Reports_2017_Daraio_et_al._SNAP-25b-deficiency_increases_insulin_secretion_and_changes_spatiotemporal_profile_of_Ca2oscillati.pdf (3,80 MB)
 http://www.nature.com/articles/s41598-017-08082-y
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| Jezik: | Angleški jezik |
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| Vrsta gradiva: | Znanstveno delo (r2) |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: | MF - Medicinska fakulteta |
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| Opis: | SNAP-25 is a protein of the core SNARE complex mediating stimulus-dependent release of insulin from pancreatic $\beta$ cells. The protein exists as two alternatively spliced isoforms, SNAP-25a and SNAP-25b, differing in 9 out of 206 amino acids, yet their specific roles in pancreatic $\beta$ cells remain unclear. We explored the effect of SNAP-25b-deficiency on glucose-stimulated insulin release in islets and found increased secretion both in vivo and in vitro. However, slow photo-release of caged $Ca^{2+}$ in $\beta$ cells within pancreatic slices showed no significant differences in $Ca^{2+}$-sensitivity, amplitude or rate of exocytosis between SNAP-25b-deficient and wild-type littermates. Therefore, we next investigated if $Ca^{2+}$ handling was affected in glucose-stimulated [beta] cells using intracellular $Ca^{2+}$-imaging and found premature activation and delayed termination of [$Ca^{2+}$] i elevations. These findings were accompanied by less synchronized $Ca^{2+}$-oscillations and hence more segregated functional $\beta$ cell networks in SNAP-25b-deficient mice. Islet gross morphology and architecture were maintained in mutant mice, although sex specific compensatory changes were observed. Thus, our study proposes that SNAP-25b in pancreatic [beta] cells, except for participating in the core SNARE complex, is necessary for accurate regulation of $Ca^{2+}$-dynamics. |
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| Ključne besede: | insulin secretion, pre-diabetes |
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| Leto izida: | 2017 |
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| Št. strani: | str. 1-14 |
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| Številčenje: | Letn. 7 |
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| ISSN: | 2045-2322 |
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| UDK: | 612.349.7 |
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| COBISS_ID: | 512737080  |
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| DOI: | 10.1038/s41598-017-08082-y |
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| ISSN pri članku: | 2045-2322 |
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| Licenca: | 
To delo je dosegljivo pod licenco Creative Commons Priznanje avtorstva 4.0 Mednarodna |
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| Število ogledov: | 27 |
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| Število prenosov: | 0 |
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| Metapodatki: |     |
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| Področja: | Ostalo
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| | | | Skupna ocena: | (0 glasov) |
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| Vaša ocena: | Ocenjevanje je dovoljeno samo prijavljenim uporabnikom. |
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