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Naslov:SNAP-25b-deficiency increases insulin secretion and changes spatiotemporal profile of $Ca^{2+}$ oscillations in $\beta$ cell networks
Avtorji:Daraio, Teresa (Avtor)
Križančić Bombek, Lidija (Avtor)
Gosak, Marko (Avtor)
Valladolid-Acebes, Ismael (Avtor)
Skelin, Maša (Avtor)
Refai, Essam (Avtor)
Berggren, Per-Olof (Avtor)
Brismar, Kerstin (Avtor)
Rupnik, Marjan (Avtor)
Bark, Christina (Avtor)
Datoteke:.pdf Scientific_Reports_2017_Daraio_et_al._SNAP-25b-deficiency_increases_insulin_secretion_and_changes_spatiotemporal_profile_of_Ca2oscillati.pdf (3,80 MB)
 
URL http://www.nature.com/articles/s41598-017-08082-y
 
Jezik:Angleški jezik
Vrsta gradiva:Znanstveno delo (r2)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:MF - Medicinska fakulteta
Opis:SNAP-25 is a protein of the core SNARE complex mediating stimulus-dependent release of insulin from pancreatic $\beta$ cells. The protein exists as two alternatively spliced isoforms, SNAP-25a and SNAP-25b, differing in 9 out of 206 amino acids, yet their specific roles in pancreatic $\beta$ cells remain unclear. We explored the effect of SNAP-25b-deficiency on glucose-stimulated insulin release in islets and found increased secretion both in vivo and in vitro. However, slow photo-release of caged $Ca^{2+}$ in $\beta$ cells within pancreatic slices showed no significant differences in $Ca^{2+}$-sensitivity, amplitude or rate of exocytosis between SNAP-25b-deficient and wild-type littermates. Therefore, we next investigated if $Ca^{2+}$ handling was affected in glucose-stimulated [beta] cells using intracellular $Ca^{2+}$-imaging and found premature activation and delayed termination of [$Ca^{2+}$] i elevations. These findings were accompanied by less synchronized $Ca^{2+}$-oscillations and hence more segregated functional $\beta$ cell networks in SNAP-25b-deficient mice. Islet gross morphology and architecture were maintained in mutant mice, although sex specific compensatory changes were observed. Thus, our study proposes that SNAP-25b in pancreatic [beta] cells, except for participating in the core SNARE complex, is necessary for accurate regulation of $Ca^{2+}$-dynamics.
Ključne besede:insulin secretion, pre-diabetes
Leto izida:2017
Št. strani:str. 1-14
Številčenje:Letn. 7
ISSN:2045-2322
UDK:612.349.7
COBISS_ID:512737080 Povezava se odpre v novem oknu
DOI:10.1038/s41598-017-08082-y Povezava se odpre v novem oknu
ISSN pri članku:2045-2322
Licenca:CC BY 4.0
To delo je dosegljivo pod licenco Creative Commons Priznanje avtorstva 4.0 Mednarodna
Število ogledov:126
Število prenosov:11
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Gradivo je del revije

Naslov:Scientific Reports
Založnik:Nature Publishing Group
ISSN:2045-2322
COBISS.SI-ID:18727432 Novo okno

Gradivo je financirano iz projekta

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Program financ.:Raziskovalni projekti - temeljni
Številka projekta:P3-0396
Naslov:Celične in tkivne mreže
Akronim:
ID projekta:info:eu-repo/grantAgreement/ARRS/Raziskovalni%20projekti%20-%20temeljni/P3-0396

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:izločanje inzulina, prediabetes


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