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Title:Vpliv različnih aditivov v izbranih polimernih materialih na rast gram pozitivnih in gram negativnih mikroorganizmov
Authors:ID Kučuk, Nika (Author)
ID Primožič, Mateja (Mentor) More about this mentor... New window
ID Leitgeb, Maja (Comentor)
Files:.pdf UN_Kucuk_Nika_2017.pdf (2,46 MB)
MD5: DBB5C2BE5F512E795979513778C79965
PID: 20.500.12556/dkum/8e284939-aa14-4c24-878f-8a08b4fe857d
 
Language:Slovenian
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Glavni namen diplomske naloge je bil določiti tisti polimerni material, ki bo najbolj zaviral rast mikroorganizmov. Antimikrobno aktivnost izbranih polimernih materialov z dodatkom različnih koncentracij cinkovega oksida (ZnO) ter drugih aditivov (stearinska kislina) smo testirali na gram negativni bakteriji Escherichia coli in gram pozitivni bakteriji Staphylococcus aureus. Analizni postopek smo izvedli v skladu z ISO standardom 22196:2007, kjer je opisana metoda, s katero smo določili antimikrobne lastnosti izbranih polimernih materialov. Uporabili smo osem različnih materialov, od katerih so bili štirje iz polietilena (PE), ostali štirje pa iz polipropilena (PP). Izbrani polimerni materiali so vsebovali različne koncentracije ZnO ter preostalih primesi. Antimikrobno aktivnost materialov smo določevali na osnovi primerjave med številom preživelih bakterijskih kolonij na testnih vzorcih, ki so vsebovali ZnO, s številom preživelih bakterijskih kolonij na slepih vzorcih, ki niso vsebovali ZnO ali drugih primesi. Rezultati so pokazali, da ZnO in dodane primesi v vseh uporabljenih polimernih materialih uspešno inhibirajo rast in razmnoževanje gram pozitivne bakterije S. aureus. Popolna inhibicija na gram negativno bakterijo E. coli pa je bila dokazana le pri dveh izbranih polimerih. Metodo po ISO 22196:2007 smo potrdili na dveh komercialnih materialih, ki sta obdelana s tehnologijo Microban® in GermoSAFE ter zagotavljata antimikrobno učinkovitost.
Keywords:antimikrobne lastnosti, inhibicija rasti mikroorganizmov, E. coli, S. aureus, cinkov oksid, polimerni materiali, polietilen, polipropilen
Place of publishing:Maribor
Publisher:[N. Kučuk]
Year of publishing:2017
PID:20.500.12556/DKUM-67491 New window
UDC:665.652.2(043.2)
COBISS.SI-ID:21049366 New window
NUK URN:URN:SI:UM:DK:VULTN0TG
Publication date in DKUM:14.09.2017
Views:1598
Downloads:84
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:21.08.2017

Secondary language

Language:English
Title:The effect of different additives in the selected polymeric materials on the growth of gram positive and gram negative microorganisms
Abstract:The main purpose of this diploma thesis was to determine polymeric material that will most inhibit the growth of microorganisms. The antimicrobial activity of the selected materials with the addition of different concentrations of zinc oxide (ZnO) and other additives (stearic acid) on gram negative bacteria Escherichia coli and gram positive bacteria Staphylococcus aureus was tested. The analytical procedure was carried out in accordance with ISO standard 22196:2007, which describes a method for determining the antimicrobial properties on selected polymeric materials. Eight different materials, of which four were made of polyethylene (PE) and the other four of polypropylene (PP) were used. The selected polymeric materials contained different concentrations of ZnO and other additives. The antimicrobial activity of the materials on the basis of comparison between the number of viable bacteria colonies recovered from samples treated with ZnO particles, with the number of viable bacteria colonies recovered from zero samples that did not contain ZnO or other additives was determined. The results showed that ZnO and added additives in all used polymeric materials successfully inhibit the growth and reproduction of gram positive bacteria S. aureus. Complete inhibition of gram negative bacteria E. coli was proved only on two selected polymers. The ISO 22196:2007 method was confirmed on two commercial materials which provide antimicrobial efficacy, treated with technology Microban® and GermoSAFE.
Keywords:antimicrobial properties, inhibition of the growth of microorganisms, E. coli, S. aureus, zinc oxide, polymeric materials, polyethylene, polypropylene


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