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Naslov:Isolation and characterization of human articular chondrocytes from surgical waste after total knee arthroplasty (TKA)
Avtorji:Naranđa, Jakob (Avtor)
Gradišnik, Lidija (Avtor)
Gorenjak, Mario (Avtor)
Vogrin, Matjaž (Avtor)
Maver, Uroš (Avtor)
Datoteke:.pdf PeerJ_2017_Naranda_et_al._Isolation_and_characterization_of_human_articular_chondrocytes_from_surgical_waste_after_total_knee_arthroplas.pdf (42,50 MB)
 
URL https://peerj.com/articles/3079/
 
Jezik:Angleški jezik
Vrsta gradiva:Znanstveno delo (r2)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:MF - Medicinska fakulteta
Opis:BACKGROUND: Cartilage tissue engineering is a fast-evolving field of biomedical engineering, in which the chondrocytes represent the most commonly used cell type. Since research in tissue engineering always consumes a lot of cells, simple and cheap isolation methods could form a powerful basis to boost such studies and enable their faster progress to the clinics. Isolated chondrocytes can be used for autologous chondrocyte implantation in cartilage repair, and are the base for valuable models to investigate cartilage phenotype preservation, as well as enable studies of molecular features, nature and scales of cellular responses to alterations in the cartilage tissue. METHODS: Isolation and consequent cultivation of primary human adult articular chondrocytes from the surgical waste obtained during total knee arthroplasty (TKA) was performed. To evaluate the chondrogenic potential of the isolated cells, gene expression of collagen type 2 (COL2), collagen 1 (COL1) and aggrecan (ACAN) was evaluated. Immunocytochemical staining of all mentioned proteins was performed to evaluate chondrocyte specific production. RESULTS: Cartilage specific gene expression of COL2 and ACAN has been shown that the proposed protocol leads to isolation of cells with a high chondrogenic potential, possibly even specific phenotype preservation up to the second passage. COL1 expression has confirmed the tendency of the isolated cells dedifferentiation into a fibroblast-like phenotype already in the second passage, which confirms previous findings that higher passages should be used with care in cartilage tissue engineering. To evaluate the effectiveness of our approach, immunocytochemical staining of the evaluated chondrocyte specific products was performed as well. DISCUSSION: In this study, we developed a protocol for isolation and consequent cultivation of primary human adult articular chondrocytes with the desired phenotype from the surgical waste obtained during TKA. TKA is a common and very frequently performed orthopaedic surgery during which both femoral condyles are removed. The latter present the ideal source for a simple and relatively cheap isolation of chondrocytes as was confirmed in our study.
Ključne besede:aggrecan, collagen 2, gene expression, human articular chondrocytes, isolation protocol, phenotype preservation, TKA, total knee arthroplasty
Leto izida:2017
Št. strani:str. 1-20
Številčenje:Letn. 5
ISSN:2167-8359
UDK:617.583-089
COBISS_ID:512695352 Povezava se odpre v novem oknu
DOI:10.7717/peerj.3079 Povezava se odpre v novem oknu
ISSN pri članku:2167-8359
NUK URN:URN:SI:UM:DK:HIFEMFCI
Licenca:CC BY 4.0
To delo je dosegljivo pod licenco Creative Commons Priznanje avtorstva 4.0 Mednarodna
Število ogledov:388
Število prenosov:134
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Gradivo je del revije

Naslov:PeerJ
Založnik:PeerJ Inc.
ISSN:2167-8359
COBISS.SI-ID:31891929 Novo okno

Gradivo je financirano iz projekta

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Številka projekta:P3-0036
Naslov:Bio-psiho-socialni model kvalitete življenja

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:agrekan, kolagen 2, ekspresija gena, artikularni hondrociti, izolacijski protokol, ohranjanje fenotipa, TKA, totalna artroplastika kolena


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