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Naslov:Use of non-conventional cell disruption method for extraction of proteins from black yeasts
Avtorji:ID Čolnik, Maja (Avtor)
ID Primožič, Mateja (Avtor)
ID Knez, Željko (Avtor)
ID Leitgeb, Maja (Avtor)
Datoteke:.pdf Frontiers_in_Bioengineering_and_Biotechnology_2016_Colnik_et_al._Use_of_Non-Conventional_Cell_Disruption_Method_for_Extraction_of_Protei.pdf (2,13 MB)
MD5: 4C3C9BE60F6C16F194250FD079612AD4
PID: 20.500.12556/dkum/f22c92d9-01b8-4787-9a70-67f64f61e592
 
URL http://journal.frontiersin.org/article/10.3389/fbioe.2016.00033/full
 
Jezik:Angleški jezik
Vrsta gradiva:Znanstveno delo
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FKKT - Fakulteta za kemijo in kemijsko tehnologijo
Opis:The influence of pressure and treatment time on cells disruption of different black yeasts and on activities of extracted proteins using supercritical carbon dioxide process was studied. The cells of three different black yeasts Phaeotheca triangularis, Trimatostroma salinum, and Wallemia ichthyophaga were exposed to supercritical carbon dioxide (SC $CO_2$) by varying pressure at fixed temperature (35°C). The black yeasts cell walls were disrupted, and the content of the cells was spilled into the liquid medium. The impact of SC $CO_2$ conditions on secretion of enzymes and proteins from black yeast cells suspension was studied. The residual activity of the enzymes cellulase, $beta$-glucosidase, $alpha$-amylase, and protease was studied by enzymatic assay. The viability of black yeast cells was determined by measuring the optical density of the cell suspension at 600 nm. The total protein concentration in the suspension was determined on UV–Vis spectrophotometer at 595 nm. The release of intracellular and extracellular products from black yeast cells was achieved. Also, the observation by an environmental scanning electron microscopy shows major morphological changes with SC $CO_2$-treated cells. The advantages of the proposed method are in a simple use, which is also possible for heat-sensitive materials on one hand and on the other hand integration of the extraction of enzymes and their use in biocatalytical reactions.
Ključne besede:P. triangularis, W. ichtyophaga, T. salinum, supercritical carbon dioxide, enzyme activity, cells
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Leto izida:2016
Št. strani:str. 1-12
Številčenje:Letn. 4
PID:20.500.12556/DKUM-66788 Novo okno
ISSN:2296-4185
UDK:66
COBISS.SI-ID:19560726 Novo okno
DOI:10.3389/fbioe.2016.00033 Novo okno
ISSN pri članku:2296-4185
NUK URN:URN:SI:UM:DK:6BTSLNMZ
Datum objave v DKUM:10.07.2017
Število ogledov:1360
Število prenosov:378
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
Področja:Ostalo
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Skupna ocena:(0 glasov)
Vaša ocena:Ocenjevanje je dovoljeno samo prijavljenim uporabnikom.
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Gradivo je del revije

Naslov:Frontiers in bioengineering and biotechnology
Skrajšan naslov:Front. bioeng. biotechnol.
Založnik:Frontiers Editorial Office
ISSN:2296-4185
COBISS.SI-ID:523093017 Novo okno

Gradivo je financirano iz projekta

Financer:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:J2-4232
Naslov:Magnetni nanodelci kot potencialni nosilci biološko aktivnih substanc

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:10.07.2017

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:P. triangularis, W. ichtyophaga, T. salinum, superkritični ogljikov dioksid, aktivnost encimov, celice


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