| Title: | Protein adsorption on various plasma-treated polyethylene-terephthalate substrates |
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| Authors: | Recek, Nina (Author)
Jaganjac, Morana (Author)
Kolar, Metod (Author)
Milković, Lidija (Author)
Mozetič, Miran (Author)
Stana-Kleinschek, Karin (Author)
Vesel, Alenka (Author) |
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| Files: |  Molecules_2013_Recek_et_al._Protein_Adsorption_on_Various_Plasma-Treated_Polyethylene_Terephthalate_Substrates.pdf (2,39 MB)
MD5: EB2F9C62655A876BA5973F3DACBD291E
 http://www.mdpi.com/1420-3049/18/10/12441/
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| Language: | English |
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| Work type: | Scientific work (r2) |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FS - Faculty of Mechanical Engineering
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| Abstract: | Protein adhesion and cell response to plasma-treated polymer surfaces were studied. The polymer polyethylene terephthalate (PET) was treated in either an oxygen plasma to make the surface hydrophilic, or a tetrafluoromethane CF4 plasma to make the surface hydrophobic. The plasma source was radiofrequency (RF) discharge. The adsorption of albumin and other proteins from a cell-culture medium onto these surfaces was studied using a quartz crystal microbalance (QCM), X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The cellular response to plasma-treated surfaces was studied as well using an MTT assay and scanning electron microscopy (SEM). The fastest adsorption rate was found on the hydrophilic oxygen plasma-treated sample, and the lowest was found on the pristine untreated sample. Additionally, the amount of adsorbed proteins was higher for the oxygen-plasma-treated surface, and the adsorbed layer was more viscoelastic. In addition, cell adhesion studies support this finding because the best cell adhesion was observed on oxygen-plasma-treated substrates. |
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| Keywords: | oxygen and fluorine plasma treatment, polymer surface modification, protein adsorption, cell adhesion, quartz crystal microbalance, QCM |
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| Year of publishing: | 2013 |
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| Number of pages: | str. 12441-12463 |
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| Numbering: | št. 10, Letn. 18 |
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| ISSN: | 1420-3049 |
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| UDC: | 533 |
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| ISSN on article: | 1420-3049 |
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| COBISS_ID: | 27136551  |
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| DOI: | 10.3390/molecules181012441 |
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| NUK URN: | URN:SI:UM:DK:QQ5MC8JC |
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| Views: | 512 |
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| Downloads: | 285 |
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| Metadata: |     |
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| Categories: | Misc.
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