Title: | Protein adsorption on various plasma-treated polyethylene-terephthalate substrates |
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Authors: | ID Recek, Nina (Author) ID Jaganjac, Morana (Author) ID Kolar, Metod (Author) ID Milković, Lidija (Author) ID Mozetič, Miran (Author) ID Stana-Kleinschek, Karin (Author) ID Vesel, Alenka (Author) |
Files: | Molecules_2013_Recek_et_al._Protein_Adsorption_on_Various_Plasma-Treated_Polyethylene_Terephthalate_Substrates.pdf (2,39 MB) MD5: EB2F9C62655A876BA5973F3DACBD291E
http://www.mdpi.com/1420-3049/18/10/12441/
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Language: | English |
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Work type: | Scientific work (r2) |
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Typology: | 1.01 - Original Scientific Article |
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Organization: | FS - Faculty of Mechanical Engineering
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Abstract: | Protein adhesion and cell response to plasma-treated polymer surfaces were studied. The polymer polyethylene terephthalate (PET) was treated in either an oxygen plasma to make the surface hydrophilic, or a tetrafluoromethane CF4 plasma to make the surface hydrophobic. The plasma source was radiofrequency (RF) discharge. The adsorption of albumin and other proteins from a cell-culture medium onto these surfaces was studied using a quartz crystal microbalance (QCM), X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The cellular response to plasma-treated surfaces was studied as well using an MTT assay and scanning electron microscopy (SEM). The fastest adsorption rate was found on the hydrophilic oxygen plasma-treated sample, and the lowest was found on the pristine untreated sample. Additionally, the amount of adsorbed proteins was higher for the oxygen-plasma-treated surface, and the adsorbed layer was more viscoelastic. In addition, cell adhesion studies support this finding because the best cell adhesion was observed on oxygen-plasma-treated substrates. |
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Keywords: | oxygen and fluorine plasma treatment, polymer surface modification, protein adsorption, cell adhesion, quartz crystal microbalance, QCM |
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Year of publishing: | 2013 |
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Publication status in journal: | Published |
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Article version: | Publisher's version of article |
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Number of pages: | str. 12441-12463 |
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Numbering: | št. 10, Letn. 18 |
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ISSN: | 1420-3049 |
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UDC: | 533 |
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ISSN on article: | 1420-3049 |
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COBISS.SI-ID: | 27136551  |
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DOI: | 10.3390/molecules181012441  |
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NUK URN: | URN:SI:UM:DK:QQ5MC8JC |
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Publication date in DKUM: | 22.06.2017 |
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Views: | 732 |
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Downloads: | 332 |
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Metadata: |  |
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Categories: | Misc.
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