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Title:The analysis of intracellular and intercellular calcium signaling in human anterior lens capsule epithelial cells with regard to different types and stages of the cataract
Authors:ID Gosak, Marko (Author)
ID Markovič, Rene (Author)
ID Fajmut, Aleš (Author)
ID Marhl, Marko (Author)
ID Hawlina, Marko (Author)
ID Andjelić, Sofija (Author)
Files:.pdf PLOS_ONE_2015_Gosak_et_al._The_Analysis_of_Intracellular_and_Intercellular_Calcium_Signaling_in_Human_Anterior_Lens_Capsule_Epithelial_C.PDF (1,45 MB)
MD5: 9CD7AF39C6B4FB3D02B2294E46980A15
URL http://dx.plos.org/10.1371/journal.pone.0143781
Work type:Scientific work
Typology:1.01 - Original Scientific Article
Organization:MF - Faculty of Medicine
Abstract:In this work we investigated how modifications of the Ca2+ homeostasis in anterior lens epithelial cells (LECs) are associated with different types of cataract (cortical or nuclear) and how the progression of the cataract (mild or moderate) affects the Ca2+ signaling. We systematically analyzed different aspects of intra- and inter-cellular Ca2+ signaling in the human LECs, which are attached to surgically isolated lens capsule (LC), obtained during cataract surgery. We monitored the temporal and spatial changes in intracellular Ca2+ concentration after stimulation with acetylcholine by means of Fura-2 fluorescence captured with an inverted microscope. In our analysis we compared the features of Ca2+ signals in individual cells, synchronized activations, spatio-temporal grouping and the nature of intercellular communication between LECs. The latter was assessed by using the methodologies of the complex network theory. Our results point out that at the level of individual cells there are no significant differences when comparing the features of the signals with regard either to the type or the stage of the cataract. On the other hand, noticeable differences are observed at the multicellular level, despite inter-capsule variability. LCs associated with more developed cataracts were found to exhibit a slower collective response to stimulation, a less pronounced spatio-temporal clustering of LECs with similar signaling characteristics. The reconstructed intercellular networks were found to be sparser and more segregated than in LCs associated with mild cataracts. Moreover, we show that spontaneously active LECs often operate in localized groups with quite well aligned Ca2+ activity. The presence of spontaneous activity was also found to affect the stimulated Ca2+ responses of individual cells. Our findings indicate that the cataract progression entails the impairment of intercellular signaling thereby suggesting the functional importance of altered Ca2+ signaling of LECs in cataractogenesis.
Keywords:intracellular calcium signaling, anterior lens, epithelial cells, cataract
Publication status in journal:Published
Article version:Publisher's version of article
Year of publishing:2015
Numbering:Letn. 10, št. 12
PID:20.500.12556/DKUM-66301 New window
ISSN on article:1932-6203
COBISS.SI-ID:2645676 New window
DOI:10.1371/journal.pone.0143781 New window
Publication date in DKUM:19.06.2017
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Record is a part of a journal

Title:PloS one
Publisher:Public Library of Science
COBISS.SI-ID:2005896 New window

Document is financed by a project

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Project number:P3-0333
Name:Očesne bolezni odraslih in otrok


License:CC BY 4.0, Creative Commons Attribution 4.0 International
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:19.06.2017

Secondary language

Keywords:medcelični prenos kalcija, leča, epitelijske celice, katarakta


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