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Naslov:In vitro selection and characterization of new probiotic candidates from table olive microbiota
Avtorji:Botta, Cristian (Avtor)
Langerholc, Tomaž (Avtor)
Cencič, Avrelija (Avtor)
Cocolin, Luca (Avtor)
Datoteke:.pdf PLoS_ONE_2014_Botta_et_al._In_Vitro_Selection_and_Characterization_of_New_Probiotic_Candidates_from_Table_Olive_Microbiota.PDF (1,63 MB)
MD5: B0B93E3C1E5060494E49FA7BD1A32A0B
 
URL http://dx.plos.org/10.1371/journal.pone.0094457
 
Jezik:Angleški jezik
Vrsta gradiva:Znanstveno delo (r2)
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FKBV - Fakulteta za kmetijstvo in biosistemske vede
Opis:To date, only a few studies have investigated the complex microbiota of table olives in order to identify new probiotic microorganisms, even though this food matrix has been shown to be a suitable source of beneficial lactic acid bacteria (LAB). Two hundred and thirty eight LAB, belonging to Lactobacillus plantarum, Lactobacillus pentosus and Leuconostoc mesenteroides species, and isolated from Nocellara Etnea table olives, have been screened in this survey through an in vitro approach. A simulation of transit tolerance in the upper human gastrointestinal tract, together with autoaggregation and hydrophobicity, have been decisive in reducing the number of LAB to 17 promising probiotics. None of the selected strains showed intrinsic resistances towards a broad spectrum of antibiotics and were therefore accurately characterized on an undifferentiated and 3D functional model of the human intestinal tract made up of H4-1 epithelial cells. As far as the potential colonization of the intestinal tract is concerned, a high adhesion ratio was observed for Lb. plantarum O2T60C (over 9%) when tested in the 3D functional model, which closely mimics real intestinal conditions. The stimulation properties towards the epithelial barrier integrity and the in vitro inhibition of L. monocytogenes adhesion and invasion have also been assessed. Lb. plantarum S1T10A and S11T3E enhanced trans-epithelial electrical resistance (TEER) and therefore the integrity of the polarized epithelium in the 3D model. Moreover, S11T3E showed the ability to inhibit L. monocytogenes invasion in the undifferentiated epithelial model. The reduction in L. monocytogenes infection, together with the potential enhancement of barrier integrity and an adhesion ratio that was above the average in the 3D functional model (6.9%) would seem to suggest the Lb. plantarum S11T3E strain as the most interesting candidate for possible in vivo animal and human trials.
Ključne besede:antibiotics, bacteria pathogen, cell metabolism, olives, probiotics, digestion
Leto izida:2014
Št. strani:str. 1-15
Številčenje:št. 4, Letn. 9
ISSN:1932-6203
UDK:579
COBISS_ID:3702316 Novo okno
DOI:10.1371/journal.pone.0094457 Novo okno
ISSN pri članku:1932-6203
NUK URN:URN:SI:UM:DK:BANLNWBP
Število ogledov:587
Število prenosov:274
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
Področja:Ostalo
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Gradivo je del revije

Naslov:PloS one
Založnik:Public Library of Science
ISSN:1932-6203
COBISS.SI-ID:2005896 Novo okno

Gradivo je financirano iz projekta

Financer:EC - European Commission
Program financ.:FP7
Številka projekta:243471
Naslov:Table olive fermentation with selected strains of probiotic lactic acid bacteria. Towards a new functional food.
Akronim:PROBIOLIVES

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:19.06.2017

Sekundarni jezik

Jezik:Slovenski jezik
Ključne besede:antibiotiki, patogena bakterija, celični metabolizem, olive, probiotiki, prebava


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