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Title:Extraction and separation of active compounds by supercritical fluids extraction and preparative supercritical chromatography
Authors:ID Bartolome Ortega, Alejandro (Author)
ID Knez, Željko (Mentor) More about this mentor... New window
ID Škerget, Mojca (Co-mentor)
Files:.pdf DOK_Bartolome_Ortega_Alejandro_2017.pdf (3,20 MB)
MD5: C3D59723CCC6C46210A016688547A5F4
 
Language:English
Work type:Doctoral dissertation
Typology:2.08 - Doctoral Dissertation
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:This doctoral dissertation is focused on the benefits of supercritical fluids technology in several industry processes. Although, supercritical CO2 is currently the most employed supercritical fluid at laboratory scale, its presence in the industry field is still limited, due to the necessity of high investment costs. Despite that fact, operations with supercritical CO2 are cheap, environmentally friendly and simple, which make it an excellent alternative to organic compounds. Extraction and separation of highly pure active compounds, such as xanthohumol or beta-sitosterol, are demanded for pharmaceutical or food industries, which create an excellent opportunity for supercritical CO2 operations, since they are solvent free. This manuscript is divided into three different parts. In the first part, conventional extraction processes and supercritical fluid process are theoretically compared and supercritical fluid extraction from saw palmetto berries is presented. In the second part, conventional chromatographic techniques and supercritical fluid chromatography are theoretically compared and separation of xanthohumol from hop extracts by supercritical fluid chromatography is presented. In the last part, different encapsulation and emulsification processes are listed and defined. These processes are very important for food and pharmaceutical companies, since several active compounds are not water soluble which create the necessity of different formulations in order to be solved in an appropriate media. Regarding the experimental part, extractions from saw palmetto berries were performed at various pressures and temperatures, which were higher than the ones that were applied in previous studies, in order to estimate and calculate different parameters (solubility, extraction kinetic curves, composition,…), and to compare different studies. Separation of xanthohumol was carried out in a homemade supercritical chromatography, which manageable variables were temperature, pressure, mass flow-rate, modifier, and stationary phase. The results were compared with those ones which were obtained using conventional techniques, in order to prove the suitability and the improvement of this technique against others tecniques.
Keywords:supercritical CO2, supercritical fluid extraction, supercritical fluid chromatography, xanthohumol, saw palmetto berries, Beta-Sitosterol, encapsulation
Place of publishing:Maribor
Publisher:A. Bartolomé Ortega
Year of publishing:2017
PID:20.500.12556/DKUM-65591 New window
UDC:664.1.033:66.081.1(043.3)
COBISS.SI-ID:20672278 New window
NUK URN:URN:SI:UM:DK:24RBAQRV
Publication date in DKUM:13.07.2017
Views:2013
Downloads:243
Metadata:XML RDF-CHPDL DC-XML DC-RDF
Categories:KTFMB - FKKT
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Secondary language

Language:Slovenian
Title:Ekstrakcija in separacija aktivnih spojin z nadkritično ekstrakcijo in preparativno nadkritično kromatografijo
Abstract:V okviru doktorske disertacije smo predstavili koristi tehnologij s superkritičnimi fluidi v različnih industrijskih procesih. Čeprav je superkritični CO2 trenutno eden najpogosteje zastopanih superkritičnih fluidov v laboratorijskem merilu, pa je njegova prisotnost na področju industrije še vedno omejena, saj takšna vrsta tehnologije zahteva potrebe po zelo visokih investicijskih stroških. Kljub temu so procesi, kjer se uporablja superkritični CO2 preprosti, okolju prijazni, cena CO2 pa relativno nizka. Takšni procesi predstavljajo odlično alternativo organskim topilom. Postopki ekstrakcije in ločevanja zelo čistih aktivnih substanc, kot sta ksantohumol in beta-sitosterol za zahteve bodisi farmacevtske bodisi prehrambne industrije, kažejo odlično priložnost uporabe procesov s superkritičnim CO2 brez uporabe organskih topil. Doktorska disertacija je razdeljena na tri različne dele. V prvem delu smo raziskovali konvencionalne ekstrakcije in ekstrakcije s superkritičnimi fluidi iz jagod žago-listne palme. V nadaljevanju smo predstavili ter med seboj primerjali konvencionalne kromatografske tehnike s superkritično kromatografijo. Uspešno smo s pomočjo superkritične kromatografije ločili ksantohumol iz hmeljskega ekstrakta. V zadnjem delu doktorske disertacije smo predstavili različne tehnike emulgiranja in enkapsulacije. Ti procesi so zelo pomembni v prehrambni in farmacevtski indistriji, saj je večina aktivnih komponent netopna v vodi. Takšni procesi omogočajo proizvodnjo takšnih formulacij, ki se nato topijo v ustreznih medijih. Eksperimentalni del doktorske disertacije obsega ekstrakcije iz jagod žago-listne palme pri višjih obratopvnih pogojih kot so bili uporabljeni v predhodnih študijah. Ločevanje ksantohumola smo izvedli na pilotni napravi za superkritično kromatografijo, kjer smo spreminjali temperaturo, tlak, masni pretok, mobilno in stacionarno fazo. Rezultate smo nato primerjali s tistimi pridobljenimi s konvencionalnimi tehnikami. Skušali smo dokazati prednosti uporabe superkritične kromatografije pred drugimi tehnikami.
Keywords:superkritični CO2, superkritična ekstrakcija, superkritična kromatografija, ksantohumol, jagode žago-listne palme, beta-sitosterol, enkapsulacija


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