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Title:Comparison of methods for detection of four common nosocomial pathogens on hospital textiles
Authors:Fijan, Sabina (Author)
Šostar-Turk, Sonja (Author)
Rozman, Urška (Author)
Files:.pdf Zdravstveno_varstvo_2014_Fijan,_Turk,_Rozman_Comparison_of_methods_for_detection_of_four_common_nosocomial_pathogens_on_hospital_textile.pdf (811,70 KB)
 
URL http://www.degruyter.com/view/j/sjph.2014.53.issue-1/sjph-2014-0003/sjph-2014-0003.xml
 
Language:English
Work type:Scientific work (r2)
Typology:1.01 - Original Scientific Article
Organization:FZV - Faculty of Health Sciences
Abstract:Introduction: Although the most common vehicle for transmission of health-care acquired infections is the personto- person transmission route, the role of environment should not be ignored and hospital linen may contribute to the spreading of nosocomial infections. The contact plate method and swabbing are common methods for sampling microorganisms on textiles; however, results are available after two days as they are based on incubation followed by phenotypeidentification. An important alternative is using quick wash-off methods followed by PCR detection, which shortens the identification process from two days to a few hours. Methods: The following test microorganisms at different concentrations were inoculated onto textile swatches and dried overnight: Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and Clostridium difficile. RODAC plate sampling as well as a non-destructive wash-off method for capturing microorganisms from the textilesusing a Morapex device were used. The elution suspension from the Morapex device was used for two methods. In the first method, classical incubation on selective media followed by phenotypic identification was used and in the second method DNA was extracted from the elution suspension followed by amplification and agarose gel electrophoresis to visualize amplified products. Conclusions: All chosen bacteria were found using all methods. However, the most sensitive proved to be detection using PCR amplification as we detected the sample with initial concentration of 102 cfu/mL inoculated onto the textile surface before drying. The final detectablerecovered bacterial concentration on textiles was up to 10 cfu/mL.
Keywords:health care associated infections, hospital textiles, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Clostridium difficile, Morapex
Year of publishing:2014
Number of pages:str. 17-25
Numbering:št. 1, Letn. 53
ISSN:0351-0026
UDC:616.9:677.021
ISSN on article:0351-0026
COBISS_ID:1966756 Link is opened in a new window
DOI:10.2478/sjph-2014-0003 Link is opened in a new window
NUK URN:URN:SI:UM:DK:ICMI5EU5
License:CC BY-NC-ND 4.0
This work is available under this license: Creative Commons Attribution Non-Commercial No Derivatives 4.0 International
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Downloads:211
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Record is a part of a journal

Title:Zdravstveno varstvo
Shortened title:Zdr. varst.
Publisher:De Gruyter Open
ISSN:0351-0026
COBISS.SI-ID:3287810 New window

Document is financed by a project

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Project no.:1000-10-310152
Name:

Secondary language

Language:Slovenian
Title:Primerjava metod za detekcijo štirih pogostih povzročiteljev bolnišničnih okužb na bolnišničnih tekstilijah
Abstract:Izhodišča: Čeprav so bolniki ali osebje najpogostejši vir bolnišnično pridobljenih okužb, ne smemo zanemariti vloge okolja, med katere sodijo bolnišnične tekstilije, ki lahko prispevajo k širitvi bolnišničnih okužb. Najpogostejši metodi vzorčenja mikroorganizmov na tekstilijah sta vzorčenje s kontaktnimi ploščami in z brisi, vendar so izsledki na voljo čez dva dni, ker so osnovani na fenotipski identifikaciji po inkubaciji vzorca na gojiščih. Pomembna alternativa so metode eluiranja, ki jim sledi detekcija s PCR-metodo,kar skrajša proces identifikacije z dveh dni na nekaj ur. Metode: Na tekstilne vzorce smo pri različnih koncentracijah nanesli naslednje izbranemikroorganizme - Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa in Clostridium difficile - in jih sušili čez noč. Iz tekstilnih vzorcev smo nato zajeli mikroorganizme z vzorčenjem sploščicami RODAC kakor tudi z nedestruktivno metodo eluiranja z aparatom Morapex. Eluirano suspenzijo iz aparata Morapex smo nadalje uporabili za dve metodi. Pri prvi metodi smo eluat inkubirali na selektivna gojišča, čemur je sledila fenotipska identifikacija, pri drugi metodi pa smo iz eluata ekstrahirali DNK in ga pomnožili ter nato s pomnoženimi produkti izvedli agarozno gelsko elektroforezo. Zaključki: Vse mikroorganizme smo zaznali z vsemi metodami, vendar je bila najobčutljivejša metoda pomnoževanje DNK s PCR-metodo, saj smo po inkubaciji zaznali tudi vzorec z začetno koncentracijo 102 cfu/mL bakterijske suspenzije, inokulirane na tekstilno površino pred sušenjem. Končna koncentracija bakterijske suspenzije, ki smo jo zaznali na tekstilnem vzorcu, je bila do 10 cfu/mL.
Keywords:bolnišnično pridobljene okužbe, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Clostridium difficile, Morapex


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