| | SLO | ENG | Cookies and privacy

Bigger font | Smaller font

Show document

Title:Vpliv velikosti vcepka in gojišča na regeneracijo brstov rebrinčevolistne hladnikovke (Hladnikia pastinacifolia Rchb.) v tkivni kulturi
Authors:Mujakić, Jakup (Author)
Ambrožič-Dolinšek, Jana (Mentor) More about this mentor... New window
Ciringer, Terezija (Co-mentor)
Files:.pdf UN_Mujakic_Jakup_2016.pdf (1,86 MB)
MD5: 2B526BDE789A10213680834AAF66B26E
 
Language:Slovenian
Work type:Undergraduate thesis (m5)
Typology:2.11 - Undergraduate Thesis
Organization:FNM - Faculty of Natural Sciences and Mathematics
Abstract:Rebrinčevolistna hladnikovka (Hladnikia pastinacifolia Rchb.) je redka in potencialno ogrožena endemična vrsta, vključena v program krioprezervacije. Cilj diplomske naloge je določiti najbolj optimalno velikost vcepka, ki bi bila primerna za postopke krioprezervacije in najprimernejša gojišča, za regeneracijo takšnih vcepkov. Raziskali smo tudi učinek predpriprave pri 5 °C na regeneracijo vcepkov. Uporabili smo tri različne velikosti vcepkov brez predhodne krioprezervacije, in sicer majhne (0,5–1 mm), srednje (1–2 mm) in velike (2–3 mm). Le-te smo vcepili na tri različna gojišča, ki jih uporabljamo pri postopkih mikropropagacije, in sicer osnovno MS; osnovno MS z 2 μM BAP (6-benzilamino purin); osnovno MS z 5 μM BAP in 3 μM IBA (indol–3–maslena kislina). Velikost vcepka je vplivala na preživetje in ponovno rast vcepkov. Najboljšo regeneracijo v brste smo dosegli pri velikih vcepkih na osnovnem gojišču MS, medtem ko smo najslabše rezultate dobili pri malih vcepkih. Vcepki na gojiščih z dodanimi rastnimi regulatorji so večinoma regenerirali v kalus ali propadli. Predpriprava je pozitivno vplivala na preživetje vcepkov, vendar je učinke predpriprave potrebno še dodatno raziskati.
Keywords:Hladnikia pastinacifolia, Apiaceae, tkivna kultura, krioprezervacija, velikost vcepka vršiček poganjka
Year of publishing:2016
Publisher:[J. Mujakić]
Source:Maribor
UDC:57(043.2)
COBISS_ID:22439944 New window
NUK URN:URN:SI:UM:DK:FWNGG91M
Views:1103
Downloads:73
Metadata:XML RDF-CHPDL DC-XML DC-RDF
Categories:FNM
:
  
Average score:(0 votes)
Your score:Voting is allowed only for logged in users.
Share:AddThis
AddThis uses cookies that require your consent. Edit consent...

Hover the mouse pointer over a document title to show the abstract or click on the title to get all document metadata.

Secondary language

Language:English
Title:The effect of explant size and growth media on regeneration of Hladnikia pastinacifolia Rchb. in tissue culture
Abstract:Hladnikia pastinacifolia Rchb. is a rare and potentially endangered endemic species, involved in the program of cryopreservation. The purpose of the project is to investigate the influence of different culture media on the regeneration of different sizes of explants. Our goal is to determine the optimal size of the explant, which would still be appropriate for the procedures of cryopreservation. We are also investigating the most suitable culture medium for the regeneration of shoot tips. We have also studied the effects of pretreatment in a cool place at 5°C. We have used three different sizes of shoot tips: small (0,5–1 mm), medium (1–2 mm) and large (2–3 mm), which were moved to the different culture media, namely a basic MS medium; MS medium with added 2μM BAP (6-benzylaminopurine) and MS medium with added 5μM BAP and 3μM IBA (indole-3-butyric acid). The size of the explant is effecting on the survival and regrowth of them. The best survival and regrowth rate was achieved in large explants with basic MS medium, while small ones gave the worst results. Explants cultured on medium with added growth regulators usually regenerated callus or they died. Pretreatment had also a positive impact on survival of explants but it needs to be further investigated.
Keywords:Hladnikia pastinacifolia, Apiaceae, tissue culture, cryopreservation


Comments

Leave comment

You have to log in to leave a comment.

Comments (0)
0 - 0 / 0
 
There are no comments!

Back
Logos of partners University of Maribor University of Ljubljana University of Primorska University of Nova Gorica