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Title:Membrane potential and calcium dynamics in beta cells from mouse pancreas tissue slices : theory, experimentation, and analysis
Authors:Rupnik, Marjan (Author)
Špelič, Denis (Author)
Gosak, Marko (Author)
Skelin, Maša (Author)
Žalik, Borut (Author)
Dolenšek, Jurij (Author)
Stožer, Andraž (Author)
Files:.pdf Sensors_2015_Dolensek_et_al._Membrane_Potential_and_Calcium_Dynamics_in_Beta_Cells_from_Mouse_Pancreas_Tissue_Slices_Theory,_Experimenta.pdf (4,17 MB)
Work type:Scientific work (r2)
Typology:1.02 - Review Article
Organization:MF - Faculty of Medicine
Abstract:Beta cells in the pancreatic islets of Langerhans are precise biological sensors for glucose and play a central role in balancing the organism between catabolic and anabolic needs. A hallmark of the beta cell response to glucose are oscillatory changes of membrane potential that are tightly coupled with oscillatory changes in intracellular calcium concentration which, in turn, elicit oscillations of insulin secretion. Both membrane potential and calcium changes spread from one beta cell to the other in a wave-like manner. In order to assess the properties of the abovementioned responses to physiological and pathological stimuli, the main challenge remains how to effectively measure membrane potential and calcium changes at the same time with high spatial and temporal resolution, and also in as many cells as possible. To date, the most wide-spread approach has employed the electrophysiological patch-clamp method to monitor membrane potential changes. Inherently, this technique has many advantages, such as a direct contact with the cell and a high temporal resolution. However, it allows one to assess information from a single cell only. In some instances, this technique has been used in conjunction with CCD camera-based imaging, offering the opportunity to simultaneously monitor membrane potential and calcium changes, but not in the same cells and not with a reliable cellular or subcellular spatial resolution. Recently, a novel family of highly-sensitive membrane potential reporter dyes in combination with high temporal and spatial confocal calcium imaging allows for simultaneously detecting membrane potential and calcium changes in many cells at a time. Since the signals yielded from both types of reporter dyes are inherently noisy, we have developed complex methods of data denoising that permit for visualization and pixel-wise analysis of signals. Combining the experimental approach of high-resolution imaging with the advanced analysis of noisy data enables novel physiological insights and reassessment of current concepts in unprecedented detail.
Keywords:calcium sensors, membrane potential sensors, calcium imaging, membrane potential imaging, beta cell, pancreas, denoising, patch-clamp
Year of publishing:2015
Number of pages:str. 27393-27419
Numbering:št. 11, Letn. 15
ISSN on article:1424-8220
COBISS_ID:512558136 Link is opened in a new window
DOI:10.3390/s151127393 Link is opened in a new window
License:CC BY 4.0
This work is available under this license: Creative Commons Attribution 4.0 International
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Record is a part of a journal

Shortened title:Sensors
COBISS.SI-ID:10176278 New window

Document is financed by a project

Funder:ARRS - Agencija za raziskovalno dejavnost Republike Slovenije (ARRS)
Funding Programme:Raziskovalni projekti - temeljni
Project no.:P3-0396
Name:Celične in tkivne mreže

Secondary language

Keywords:senzorji, kalcij, membranski potencial, beta celice, trebušna slinavka, razšumljanje, metoda patch-clamp


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