| Naslov: | Determination of topiramate in human plasma using liquid chromatography tandem mass spectrometry |
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| Avtorji: | ID Vnučec, Tanja (Avtor)
ID Cvitkovič-Maričič, Lea (Avtor)
ID Prosen, Helena (Avtor)
ID Brodnjak-Vončina, Darinka (Avtor) |
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| Datoteke: |  Acta_Chimica_Slovenica_2013_Popov_et_al._Determination_of_topiramate_in_human_plasma_using_liquid_chromatography_tandem_mass_spectrometr.pdf (189,19 KB)
MD5: 2BCBAE1A3F84F1CB92F1331CAC98A245
 http://acta-arhiv.chem-soc.si/60/60-1-144.pdf
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| Jezik: | Angleški jezik |
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| Vrsta gradiva: | Znanstveno delo |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: | FKKT - Fakulteta za kemijo in kemijsko tehnologijo
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| Opis: | The LC-MS/MS method for determination of the anti-epileptic drug topiramate (TPM) in human plasma was developed and validated for pharmacokinetic and bioequivalence study purposes. For quantitative determination of TPM values the method with deuterated internal standard (topiramate-d12) and liquid chromatography with tandem mass spectrometry was used. TPM was extracted from the human plasma using the solid-phase extraction procedure on a Strata X extraction column. Negative ions were monitored in the selected reaction monitoring mode (SRM) and transitions m/z 338.2 > 78.2 and m/z 350.3 > 78.2 were used for the quantitative evaluation of TPM and the internal standard, respectively. The results obtained from validation were statistically evaluated according to the requirements of European Medicines Agency (EMA) and Food and Drug Administration (FDA) regulatory guidelines. The linearity of the method was checked within a concentration range from 10 to 2000 ng/mL. Successful validation confirmed that this method is precise, accurate, sensitive and therefore suitable for determination of topiramate plasma levels in pharmacokinetic and bioequivalence studies. |
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| Ključne besede: | topiramate, liquid chromatography tandem mass spectroscopy, human plasma, bioequivalence study |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Leto izida: | 2013 |
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| Št. strani: | str. 144-150 |
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| Številčenje: | Letn. 60, št. 1 |
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| PID: | 20.500.12556/DKUM-58141  |
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| ISSN: | 1318-0207 |
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| UDK: | 543.544.5:543.51:615.07 |
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| COBISS.SI-ID: | 36618757 |
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| ISSN pri članku: | 1318-0207 |
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| NUK URN: | URN:SI:UM:DK:AXOJG6XP |
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| Datum objave v DKUM: | 18.08.2017 |
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| Število ogledov: | 1609 |
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| Število prenosov: | 104 |
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| Metapodatki: |    |
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| Področja: | Ostalo
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