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Title:Pressure stability of lipases and their use in different systems
Authors:ID Leitgeb, Maja (Author)
ID Knez, Željko (Author)
Files:.pdf Acta_Chimica_Slovenica_2001_Leitgeb,_Knez_Pressure_stability_of_lipases_and_their_use_in_different_systems.pdf (608,04 KB)
MD5: F228BB58E24D979B9A351DA996A7D6EF
URL http://acta-arhiv.chem-soc.si/48/48-4-521.pdf
Work type:Scientific work
Typology:1.01 - Original Scientific Article
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:For the investigation of the solvent impact on the enzymes, lipases from different sources (Pseudomonas fluorescences, Rhizopus javanicus, Rhizopus niveus, Candida rugose and Porcine pancreas) were used. Stability and activity of these lipases in aqueous medium in supercritical $CO_2$ and liquid propane at 100 bar and 40°C were studied. On the basis of previous results lipases were used for their application in two different systems. The application of the polysulphone membrane in the continuous stirred tank membrane reactor was studied on the model system of the hydrolysis of oleyl oleate in propane at high pressure. As a catalyst the Candida rugosa lipase was used. The next utilization of lipases was the use of on silica arerogel self-immobilized lipase from Porcine pancreas as catalyst for esterification reaction in near-critical propane at 40°C and 100 bar.
Keywords:chemical processing, supercritical fluids, lipases, enzyme stability, high pressure membrane reactor
Publication status in journal:Published
Article version:Publisher's version of article
Year of publishing:2001
Number of pages:str. 521-532
Numbering:Letn. 48, št. 4
PID:20.500.12556/DKUM-53177 New window
ISSN on article:1318-0207
COBISS.SI-ID:6781974 New window
Publication date in DKUM:10.07.2015
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Record is a part of a journal

Title:Acta Chimica Slovenica
Shortened title:Acta Chim. Slov.
Publisher:Slovensko kemijsko društvo
COBISS.SI-ID:14086149 New window


License:CC BY 4.0, Creative Commons Attribution 4.0 International
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:10.07.2015

Secondary language

Abstract:Za študijo vpliva topil na encime smo izbrali lipaze iz raznih virov (Pseudomonas fluorescences, Rhizopus javanicus, Rhizopus niveus, Candida rugosa in Prochine pancreas). Ugotavljali smo spremembe v aktivnosti oz. stabilnosti omenjenih lipaz v vodnem mediju pri atmosferskem tlaku, v superkritičnem $CO_2$ in sub-kritičnem propanu. Na osnovi dobljenih rezultatov smo lipaze uporabili kot katalizatorje v dveh različnih sistemih. Uporabo polisulfonskih membran v kontinuiranem mešalnem membranskem reaktorju smo študirali s pomočjo modelnega sistema hidrolize oleil oleata v sub-kritičnem propanu. Kot katalizator smo uporabili lipazo iz candida rugose. Druga aplikacija pa je bila uporaba Porchine pancreas lipaze, ki smo jo predhodno sami imobilizirali na silicijevem aerogelu, za katalizo esterifikacije v sub-kritičnem propanu.
Keywords:kemijska procesna tehnika, superkritični fluidi, lipaze, stabilnost encimov, visokotlačni membranski reaktor


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