| Title: | Pressure stability of lipases and their use in different systems |
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| Authors: | ID Leitgeb, Maja (Author)
ID Knez, Željko (Author) |
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| Files: |  Acta_Chimica_Slovenica_2001_Leitgeb,_Knez_Pressure_stability_of_lipases_and_their_use_in_different_systems.pdf (608,04 KB)
MD5: F228BB58E24D979B9A351DA996A7D6EF
 http://acta-arhiv.chem-soc.si/48/48-4-521.pdf
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| Language: | English |
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| Work type: | Scientific work |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FKKT - Faculty of Chemistry and Chemical Engineering
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| Abstract: | For the investigation of the solvent impact on the enzymes, lipases from different sources (Pseudomonas fluorescences, Rhizopus javanicus, Rhizopus niveus, Candida rugose and Porcine pancreas) were used. Stability and activity of these lipases in aqueous medium in supercritical $CO_2$ and liquid propane at 100 bar and 40°C were studied.
On the basis of previous results lipases were used for their application in two different systems. The application of the polysulphone membrane in the continuous stirred tank membrane reactor was studied on the model system of the hydrolysis of oleyl oleate in propane at high pressure. As a catalyst the Candida rugosa lipase was used. The next utilization of lipases was the use of on silica arerogel self-immobilized lipase from Porcine pancreas as catalyst for esterification reaction in near-critical propane at 40°C and 100 bar. |
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| Keywords: | chemical processing, supercritical fluids, lipases, enzyme stability, high pressure membrane reactor |
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| Publication status in journal: | Published |
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| Article version: | Publisher's version of article |
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| Year of publishing: | 2001 |
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| Number of pages: | str. 521-532 |
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| Numbering: | Letn. 48, št. 4 |
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| PID: | 20.500.12556/DKUM-53177  |
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| ISSN: | 1318-0207 |
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| UDC: | 66-987 |
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| ISSN on article: | 1318-0207 |
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| COBISS.SI-ID: | 6781974 |
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| NUK URN: | URN:SI:UM:DK:EYM2GW6U |
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| Publication date in DKUM: | 10.07.2015 |
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| Views: | 1243 |
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| Downloads: | 177 |
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| Metadata: |     |
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| Categories: | Misc.
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