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Title:Cell physiology in tissue slices : studying beta cells in the islets of Langerhans
Authors:ID Stožer, Andraž (Author)
ID Dolenšek, Jurij (Author)
ID Skelin, Maša (Author)
ID Rupnik, Marjan (Author)
Files:URL http://www.actamedbio.mf.uni-mb.si/03_10id_amb_97_13_v2.pdf
 
URL http://www.actamedbio.mf.uni-mb.si/03_10id_amb_97_13_v2.pdf
 
.pdf 02.pdf (1,24 MB)
MD5: FF7CB2DE6A3A66EFA86AEE48BFEE4801
 
Language:English
Work type:Scientific work
Typology:1.02 - Review Article
Organization:MF - Faculty of Medicine
Abstract:Isolated endocrine cells have enabled the development of numerous methods to assess basic cellular architecture and define a significant part of the molecular machinery involved in cellular processes, such as excitability or the exocytotic release of hormones following cytosolic calcium increase. These efforts have generated consensus models explaining the activation or general operation of a particular cell type. The use of fresh tissue slices can go even further and uncover a series of emergent properties never explained or predicted by consensus models. In this paper, we present a selection of our most important experimental findings in the beta cells of pancreatic tissue slices.
Keywords:celica beta, Langerhansov otoček, tkivna rezina trebušne slinavke miši, elektrofiziologija, konfokalna fluorescenčna mikroskopija, kalcijevo barvilo, teorija grafov
Publication status:Published
Publication version:Version of Record
Publication date:01.01.2013
Year of publishing:2013
Number of pages:str. 20-32
Numbering:Vol. 6, [no.] 1
PID:20.500.12556/DKUM-50510-bddd1c14-2b86-0184-c253-8c4b8d18634e New window
UDC:612.349.7
ISSN on article:1855-5640
COBISS.SI-ID:512298296 New window
NUK URN:URN:SI:UM:DK:9WTZPTJI
Publication date in DKUM:10.07.2015
Views:1657
Downloads:75
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Categories:Misc.
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Record is a part of a journal

Title:Acta medico-biotechnica : AMB
Publisher:Medicinska fakulteta, Medicinska fakulteta, Univerzitetna založba Univerze v Mariboru
ISSN:1855-5640
COBISS.SI-ID:242526720 New window

Secondary language

Language:Slovenian
Title:Celična fiziologija v tkivnih rezinah : preučevanje celic beta v Langerhansovih otočkih
Abstract:Uporaba izoliranih endokrinih celic je omogočila razvoj številnih metod za oceno osnovne celične arhitekture in opredelitev pomembnega dela molekularnih mehanizmov, ki sodelujejo pri celičnih procesih, kot sta vzdražnost celic in izločanje hormonov v procesu eksocitoze po porastu znotrajcelične koncentracije kalcijevih ionov. Ta prizadevanja so vodila v oblikovanje dogovornih modelov, ki razlagajo aktivacijo in splošen način delovanja določenega celičnega tipa. Z uporabo svežih tkivnih rezin lahko presežemo dosedanje raziskave in razkrijemo celo vrsto porajajočih se lastnosti, ki jih dogovorni modeli ne morejo zlahka predvideti niti razložiti. V tem članku predstavljamo izbor naših najpomembnejših eksperimentalnih ugotovitev na celicah beta v tkivnih rezinah trebušne slinavke.
Keywords:beta cell, islet of Langerhans, mouse pancreatic tissue slice, electrophysiology, confocal fluorescent microscopy, calcium dye, graph theory


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This document is a collection and includes these documents:
  1. Acta medico-biotechnica

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