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Title:Real-time polymerase chain reaction for quantitative assessment of common pathogens associated with healthcare-acquired infections on hospital textiles
Authors:Rozman, Urška (Author)
Fijan, Sabina (Author)
Šostar-Turk, Sonja (Author)
Mlakar, Vid (Author)
Files:URL http://trj.sagepub.com/content/early/2013/05/21/0040517513487792.full.pdf+html
 
Language:English
Work type:Not categorized (r6)
Typology:1.01 - Original Scientific Article
Organization:FZV - Faculty of Health Sciences
Abstract:A hospital environment may act as a significant reservoir for potential pathogens that can be transmitted with hospital textiles, which could represent a source of healthcare-acquired infections. Quantitative assessment of nosocomial pathogens with real time polymerase chain reaction (qPCR) on textiles can serve to verify the achievement of standards for textile hygiene of hospital laundry that assess the risk for acquiring hospital infection frominappropriately disinfected textiles. The aim of the study was to establish qPCR for quantitative assessment of selected common nosocomial pathogens (Clostridium difficile, Staphylococcus aureus, Klebsiella pneumoniaeand Pseudomonas aeruginosa) on hospital textiles and to compare the efficiency of the molecular method to the standard procedures for evaluating the bio burden of textiles in hospitals. This study demonstrated that presenceof nosocomial pathogens on hospital textiles can be confirmed with qPCR even where conventional techniques do not give any results. qPCR offers apossibility to confirm the presence of microorganisms in dead or viable but non-culturable states that cannot be detected by conventional sampling techniques but may still pose a hazard to public health.
Keywords:healthcare-acquired infections, hospital textiles, Clostridium difficile, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa
Year of publishing:2013
Number of pages:str. 2032-2041
Numbering:Vol. 83, Iss. 19
UDC:616-022.36:614
ISSN on article:1746-7748
COBISS_ID:1913252 Link is opened in a new window
DOI:10.1177/0040517513487792 Link is opened in a new window
NUK URN:URN:SI:UM:DK:S4Q902SF
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Downloads:77
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Record is a part of a journal

Title:Textile research journal
Publisher:Sage
ISSN:1746-7748
COBISS.SI-ID:518328601 New window

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