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Title:Efficiency of four sampling methods used to detect two common nosocomial pathogens on textiles
Authors:Rozman, Urška (Author)
Šostar-Turk, Sonja (Author)
Fijan, Sabina (Author)
Files:URL http://trj.sagepub.com/content/early/2012/05/15/0040517512445339.full.pdf+html
 
Language:English
Work type:Not categorized (r6)
Typology:1.01 - Original Scientific Article
Organization:FZV - Faculty of Health Sciences
Abstract:Detecting microorganisms on textiles is useful for many purposes, for example to determine the bioburden before laundering, assess the reduction in bacterial counts in connection with various laundry processes, or trace transfer routes in infection control investigations. Therefore a validated, reproducible and rational method is needed. For sampling microorganisms on textile surfaces the most commonly used method is the contact plate method using RODAC plates, first described by Hall and Hartnett followed by the swab sampling technique. Both methods can only capture microorganisms on the surface of the textiles while microorganisms that have penetrated into the deeper structure of the material will not be detected. In our research the contact plate method and the swabbing technique were compared with two wash-off methods. For the first wash-off method the destructive elution methodwas used, where microorganisms were eluted from the fabrics by shaking the fabrics for a certain time in an elution medium. For the fourth sampling method a nondestructive method that included a compact test device called MorapexŽ was used, which is based on forced desorption by pressing the microorganisms through the fabric without destroying the fabric. In our research, two types of microorganisms were included (Klebsiella pneumoniae andStaphylococcus aureus) that cause common nosocomial infections. The aim of this study was to compare the efficiency of the four sampling methods for detecting microorganisms on textiles and to determine the lowest concentration, which can still be detected. The percentage of microorganisms that were detected by both elution methods was substantially higher than by sampling of fabrics with the contact plate method or swabbing. It can be concluded that a nondestructive method using a modified MorapexŽ device can beapplied for quick determination of the hygienic condition of textiles.
Keywords:textile hygiene, Staphylococcus aureus, Klebsiella pneumoniae, swabbing, RODAC plates, elution method, Morapex
Year of publishing:2012
Number of pages:str. 2099-2105
Numbering:Vol. 82, no. 20
UDC:677:613
ISSN on article:1746-7748
COBISS_ID:1822372 Link is opened in a new window
DOI:10.1177/0040517512445339 Link is opened in a new window
NUK URN:URN:SI:UM:DK:UOQHYMNI
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Downloads:66
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Record is a part of a journal

Title:Textile research journal
Publisher:Sage
ISSN:1746-7748
COBISS.SI-ID:518328601 New window

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