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Title:DOLOČEVANJE MEJE DETEKCIJE IZBRANIH MIKROORGANIZMOV NA RAZLIČNIH POVRŠINAH V BOLNIŠNIČNEM OKOLJU Z METODO PCR
Authors:Murko, Patricija (Author)
Fijan, Sabina (Mentor) More about this mentor... New window
Šostar Turk, Sonja (Co-mentor)
Files:.pdf MAG_Murko_Patricija_2015.pdf (2,41 MB)
MD5: 8C50CCC7631CD36758F6A56D2FCE20EB
 
Language:Slovenian
Work type:Master's thesis/paper (mb22)
Typology:2.09 - Master's Thesis
Organization:FZV - Faculty of Health Sciences
Abstract:Izhodišča: V magistrski nalogi smo določevali mejo detekcije izbranim mikroorganizmom na različnih površinah v bolnišničnem okolju. Prenos s človeka na človeka je najbolj pogost, saj je v bolnišničnem okolju veliko pacientov, prav tako pa tudi zdravstvenega osebja. Različne površine v bolnišničnem okolju lahko tudi pripomorejo k razširitvi mikroorganizmov. Dokazano je, da so mikroorganizmi sposobni preživeti na površinah tudi do nekaj tednov. Metodologija: V raziskavi smo vzorčili predhodno kontaminirane kovinske, steklene in plastične površine in jemali brise le-teh po 24-urnem sušenju. Izbrani mikroorganizmi so bili: E. faecium, S. aureus, B. subtilis, E. coli, K. pneumoniae, P. aeruginosa in C. albicans. Iz brisov smo eluirali mikroorganizme s fiziološko raztopino. Iz eluata smo najprej določili koncentracijo mikroorganizmov s klasično mikrobiološko metodo z nanosom na selektivne medije. Z metodo PCR smo nato določili še prisotnost DNK izbranih mikroorganizmov. Za statistično analizo smo uporabili programsko orodje IBM SPSS 20. Z neparametričnima testoma smo ugotavljali statistično pomembne razlike. Rezultati: Z molekularno metodo smo rezultat dobili v nekaj urah, s klasično inkubacijsko metodo je trajalo 2–3 dni. Ko smo primerjali preživetje izbranih mikroorganizmov pri različnih koncentracijah na kovinski in stekleni površini, nismo opazili statistično značilnih razlik, saj je bil p ≥ 0,05. Ko pa smo zajeli še plastično površino, so se razlike pokazale pri po Gramu pozitivnima bakterijama E. faecium in S. aureus ter po Gramu negativnima bakterijama E. coli in P. aeruginosa, saj je bil p ≤ 0,05. Sklep: Klasična gojitvena metoda je dolgotrajna, medtem ko se metoda PCR lahko uporablja za hitro oceno čistoče površin, saj smo v nekaj urah prišli do rezultatov.
Keywords:bolnišnične okužbe, detekcija, površine, PCR, Enterococcus faecium, Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Candida albicans.
Year of publishing:2015
Publisher:[P. Murko]
Source:Maribor
UDC:616-022(043.2)
COBISS_ID:2080420 New window
NUK URN:URN:SI:UM:DK:NRL07PWY
Views:1521
Downloads:129
Metadata:XML RDF-CHPDL DC-XML DC-RDF
Categories:FZV
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Secondary language

Language:English
Title:DETERMINATION OF THE DETECTION LIMIT OF CHOSEN MICROORGANISMS ON VARIOUS SURFACES IN HOSPITAL ENVIRONMENT USING THE PCR METHOD
Abstract:Introduction: In this master's thesis, we have determined the detection limit of selected microorganisms on variable surfaces in a hospital environment. Transmission from person to person is most common, because in a hospital environment there are many patients as well as medical staff. Various surfaces in the hospital environment can contribute to the spread of microorganisms. It has been proven that micro-organisms are able to survive on surfaces up to several weeks. Methodology: In this study, we sampled previously contaminated metal, glass and plastic surfaces and took swabs after 24-hour drying. Selected microorganisms were: E. faecium, S. aureus, B. subtilis, E. coli, K. pneumoniae, P. aeruginosa, and C. albicans. Then we eluted microorganisms from the swabs with a saline solution. First we determinated the concentration of microorganisms from eluate by classical microbiological method with the application on the selective agars. Then we determined the presence of the chosen microorganisms DNA with the PCR method. We used a software tool IBM SPSS 20 for statistical analysis,. With non-parametric tests, we searched for statistically significant differences. Results: With the molecular method results were acheived within a few hours; on the other hand, results from the classic incubation method took 2 to 3 days. When we compared the survival of selected microorganisms at variable concentrations on metal and glass surface, we did not notice statistically significant differences, since p was ≥ 0.05. However, when we added plastic surface to the comparison, differences were shown in Gram-positive bacteria E. faecium and S. aureus and at the Gram-negative bacteria E. coli and P. aeruginosa (p ≤ 0.05). Conclusion: Classic cultivation method is a time-consuming method, whereas the PCR method can be used for rapid cleanliness surface evaluation, since we acheived results within a few hours.
Keywords:Nosocomial infections, detection limit, surfaces, PCR, Enterococcus faecium, Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Candida albicans.


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