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Title:DOLOČEVANJE KRVNE SLIKE S HEMATOLOŠKIMI ANALIZATORJI
Authors:Rotar, Danica (Author)
Brodnjak-Vončina, Darinka (Mentor) More about this mentor... New window
Kolar, Mitja (Co-mentor)
Files:.pdf VS_Rotar_Danica_2014.pdf (2,85 MB)
 
Language:Slovenian
Work type:Bachelor thesis/paper (mb11)
Typology:2.11 - Undergraduate Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Namen diplomske naloge je kontrola kakovosti rezultatov hematološkega analizatorja ABX Pentra XL 80, proizvajalca HORIBA Medical, ki smo jo izvajali v diagnostičnem laboratoriju Zdravstvenega doma Ormož. Ugotavljali smo točnost in natančnost meritev omenjenega hematološkega analizatorja. Nadzor kakovosti v laboratoriju je namenjen za odkrivanje, zmanjšanje in odpravo pomanjkljivosti v analitskem procesu v medicinskem laboratoriju. Kontrola kakovosti je ključnega pomena pri izboljšanju kakovosti rezultatov meritev parametrov krvne slike pri pacientih, ki jih vsak dan sporočamo zdravnikom in naročnikom. Naključnim pacientom v ZD Ormož smo odvzeli vzorce polne krvi z dodatkom antikoagulanta K3EDTA in izvedli meritve krvnih slik. Primerljivost izmerjenih rezultatov smo ugotavljali z referenčnim hematološkim analizatorjem ABX Pentra 60. Dobljene rezultate smo statistično ovrednotili s parnim t-testom in linearno regresijo. Ponovljivost smo izvajali z večkratnimi meritvami vzorcev znotraj delovnega dne. Izračunane koeficiente variacij smo primerjali s podanimi koeficienti variacij proizvajalca hematološkega analizatoja HORIBA Medical. Merjenje točnosti smo izvajali s komercialnimi kontrolnimi vzorci ABX Difftrol na dveh koncentracijskih nivojih (normalni in visoki nivo). Dobljene rezultate kontrolnih vzorcev smo statistično ovrednotili s t-testom in jih hkrati tudi vnesli v kontrolne karte, ter jih ocenili z Westgardovimi pravili. Diferencialne krvne slike levkocitov smo primerjali z mikroskopskim diferenciranjem krvnih razmazov, ki smo jih izdelali sami in pobarvali po metodi May-Grünwald Giemsa. Ugotovili smo, da statistično ovrednotenje hematoloških vrednosti s t-testom in parnim t-testom ni najbolj primerno. S pomočjo linearne regresije in kontrolnih kart smo prišli do sklepa, da je hematološki analizator dovolj točen in natančen za uporabo analize krvnih slik.
Keywords:hematološki analizator, kompletna krvna slika, kontrola kakovosti
Year of publishing:2014
Publisher:[D. Rotar]
Source:Maribor
UDC:612.111(043.2)
COBISS_ID:18242838 Link is opened in a new window
NUK URN:URN:SI:UM:DK:V4X1MPGG
Views:1065
Downloads:280
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Categories:KTFMB - FKKT
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Secondary language

Language:English
Title:DETERMINATION OF BLOOD COUNTS USING HEMATOLOGY ANALYZERS
Abstract:The subject of this diploma thesis is the quality control of ABX Pentra XL 80 Hematology Analyser (made by HORIBA Medical) results, undertaken in the diagnostic laboratory in Ormož Health Centre (Zdravstveni dom Ormož). We were establishing its accuracy, and precision of measurements. Such laboratory quality control is intended to detect, reduce and eliminate imperfections in the analytical process in a medical laboratory. Quality control is a key issue in improving the quality of patients' results, which are reported to doctors daily. Random patients in Ormož Health Centre were taken full blood samples, added K3EDTA anticoagulant and performed measurements of blood count. Comparability of the measured results was performed by a reference ABX Pentra 60 Hematology Analyser. The results were statitically processed by a two sample t-test and linear regression. Repeatability was implemented by several repeated measurements during the course of a working day. The calculated coefficient of variation was compared to the coefficient of variation given by Hematology Analyser manufacturer, HORIBA Medical. The measurement of precision was implemented by commercial control of ABX Difftrol in two levels (normal and high levels). The given results of control samples were statistically processed with a t-test and fed into control charts and then assessed with Westguard rules. Leukocyte Differential Count was compared to microscope differencial blood smears, prepared in house by using May-Grünwald Giemsa stain procedure. We have concluded that statistical processing of haematological values performed by t-test and a two sample t-test is not suitable. By applying linear regression and quality charts we have concluded that hematology analyser is sufficiently accurate and precise for use in blood count analysis.
Keywords:haematological analyzer, complete blood count, quality control


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