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Title:Vzorčenje in dokazovanje prisotnosti klebsiella pneumoniae na bolnišničnih tekstilijah z različnimi metodami
Authors:Bočkaj, Marko (Author)
Fijan, Sabina (Mentor) More about this mentor... New window
Šostar Turk, Sonja (Co-mentor)
Files:.pdf VS_Bockaj_Marko_2014.pdf (945,27 KB)
MD5: 543B67C66CD29E0A4C2CA787D3F7394E
 
Language:Slovenian
Work type:Bachelor thesis/paper (mb11)
Typology:2.11 - Undergraduate Thesis
Organization:FZV - Faculty of Health Sciences
Abstract:Bolnišnične okužbe so okužbe, povezane z zdravljenjem, bivanjem ali rehabilitacijo v bolnišnici in so najpogostejši zaplet pri zdravljenju. Najpogosteje do njih pride zaradi neupoštevanja in neustreznega izvajanja preventivnih ukrepov za preprečevanje bolnišničnih okužb, med katerimi je najpomembnejša higiena rok. Bolnišnične okužbe povzročajo mikroorganizmi, kot so bakterije, virusi, glive in paraziti. Ena od povzročiteljev takšnih okužb je bakterija Klebsiella pneumoniae, ki je najpomembnejši povzročitelj bolnišničnih okužb iz rodu Klebsiella. Okužbe z bakterijo Klebsiella pneumoniae najpogosteje prizadenejo dihala, sečila in rane. Patogeni mikroorganizmi, ki povzročajo okužbe, se pogosto zadržujejo v tekstilijah, ki se uporabljajo pri vsakdanji praksi v bolnišnici. Zato je detekcija mikroorganizmov v bolnišničnih tekstilijah pomembna pri raziskovanjih na področju obvladovanja in preprečevanja bolnišničnih okužb, ki pestijo bolnike tako dolgo, kot obstajajo bolnišnice in ostale ustanove, ki obravnavajo dlje hospitalizirane bolnike. V naši raziskavi smo primerjali tri različne metode za detekcijo bakterije Klebsiella pneumoniae na bolnišničnih tekstilijah. Najprej smo vzorčili bakterije z klasično metodo jemanja odtisov z RODAC ploščicami, nato z aparatom Morapex A in za konec še z hitrim testom za detekcijo psevdomonad in enterobakterij (Inštitut Hohenstein, Nemčija). Ugotovili smo, da je metoda, pri kateri uporabljamo aparat Morapex A, bolj učinkovita od klasične metode jemanja odtisov z RODAC ploščicami. Hiter test za odkrivanje bakterij na kontaminiranih površinah (Bacteria quick test), pa je neprimeren za uporabo v praksi, saj njegovi rezultati niso tako natančni in zanesljivi kot rezultati, pridobljeni z drugimi metodami vzorčenja.
Keywords:Bolnišnične okužbe, Klebsiella pneumoniae, Morapex A, RODAC, hitri test
Year of publishing:2014
Publisher:[M. Bočkaj]
Source:Maribor
UDC:616.9:677.021(043.2)
COBISS_ID:2039716 New window
NUK URN:URN:SI:UM:DK:MXCLUSBK
Views:4801
Downloads:246
Metadata:XML RDF-CHPDL DC-XML DC-RDF
Categories:FZV
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Secondary language

Language:English
Title:Sampling and proving the presence of klebsiella pneumoniae on hospital textiles using different methods
Abstract:Nosocomial infections are infections acquired during treatment, residence or rehabilitation in hospitals. They present the most common complication of treatment in hospitals. The most common reasons for their occurrence are ignoring and improper implementation of preventive measures of nosocomial infections, the main being hand hygiene. Nosocomial infections are caused by microorganisms, like bacteria, viruses, fungi and parasites. One of the causative organisms of these infections is also the bacteria Klebsiella pneumoniae, the most important representative of the genus Klebsiella regarding nosocomial infections. Infections with Klebsiella pneumoniae most commonly affect the respiratory system, urinary system and wounds. The pathogenic microorganisms causing the infections are commonly found in textiles, which are being used in everyday clinical practice. This is the reason why the detection of microorganisms in hospital textiles is an important factor for researching in the field of managing and preventing nosocomial infections, which trouble patients for as long as hospitals exist and all institutions, that deal with patients hospitalised for a longer time. In our study, we compared different methods for detecting Klebsiella pneumoniae in hospital textiles and found out, that the method using the Morapex A apparatus is more efficient than the classical method of sampling with RODAC plates. We have also tested the Bacteria quick test (Institute Hohenstein, Germany)for detecting Pseudomonads and Enterobacteriaceae on contaminated surfaces and came to the conclusion, that the rapid test is inappropriate for use in everyday clinical practice, because the results were not as exact and reliable as results obtained with other sampling methods.
Keywords:Nosocomial infections, Klebsiella pneumoniae, Morapex A, RODAC, rapid test


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