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Naslov:Flow cytometry of HEK 293T cells interacting with polyelectrolyte multilayer capsules containing fluorescein-labeled poly(acrylic acid) as a pH sensor
Avtorji:ID Reibetanz, Uta (Avtor)
ID Haložan, David (Avtor)
ID Brumen, Milan (Avtor)
ID Donath, Edwin (Avtor)
Datoteke:URL http://pubs3.acs.org/acs/journals/doilookup?in_doi=10.1021/bm061200r
 
Jezik:Angleški jezik
Vrsta gradiva:Neznano
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FNM - Fakulteta za naravoslovje in matematiko
Opis:Polyelectrolyte multilayer sensor capsules, 5 m in diameter, which contained fluorescein-labeled poly(acrylic acid) (PAAAF) as pH-sensitive reporter molecules, were fabricated and employed to explore their endocytotic uptake into HEK 293T cells by flow cytometry. The percentage of capsules residing in the endolysosomal compartment was estimated from the fluorescence intensity decrease caused by acidification. Capsules attached to the extracellular surface of the plasma membrane were identified by trypan blue quenching. The number of capsules in the cytoplasm was rather small, being below the detection limit of the method. The advantages of polyelectrolyte multilayer capsules are that the fluorophore is protected from interaction with cellular compartments and that the multilayer can be equipped with additional functions.
Ključne besede:biophysics, polyelectrolytes, multilayer capsules, polyelectrolyte capsules
Leto izida:2007
PID:20.500.12556/DKUM-35845 Novo okno
UDK:577.3
COBISS.SI-ID:15383304 Novo okno
ISSN pri članku:1525-7797
NUK URN:URN:SI:UM:DK:T6UWPRFP
Datum objave v DKUM:07.06.2012
Število ogledov:1639
Število prenosov:114
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
Področja:Ostalo
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Skupna ocena:(0 glasov)
Vaša ocena:Ocenjevanje je dovoljeno samo prijavljenim uporabnikom.
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Gradivo je del revije

Naslov:Biomacromolecules
Založnik:American Chemical Society
ISSN:1525-7797
COBISS.SI-ID:22445317 Novo okno

Sekundarni jezik

Jezik:Angleški jezik
Ključne besede:biofizika, polielektroliti, večplastne kapsule, polielektrolitne kapsule


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