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Naslov:A novel metalloprotease from Bacillus cereus for protein fibre processing
Avtorji:Sousa, Fernanda de (Avtor)
Jus, Suzana (Avtor)
Erbel, Anita (Avtor)
Kokol, Vanja (Avtor)
Cavaco-Paulo, Artur (Avtor)
Gübitz, Georg M. (Avtor)
Datoteke:URL http://dx.doi.org/10.1016/j.enzmictec.2006.12.017
 
Jezik:Angleški jezik
Vrsta gradiva:Neznano ()
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:FS - Fakulteta za strojništvo
Opis:A novel protease produced by Bacillus cereus grown on wool as carbon and nitrogen source was purified. B. cereus protease is a neutral metalloprotease with a molecular mass of 45.6 kDa. The optimum activity was at 45 °C and pH 7.0. The substrate specificity was assessed using oxidized insulin B-chain and synthetic peptide substrates. The cleavage of the insulin B-chain was determined to be Asn3, Leu6, His10-Leu11, Ala14, Glu21, after 12 h incubation. Among the peptide substrates, the enzyme did not exhibit activity towards ester substrates; with p-nitroanilide, the kinetic data indicate that aliphatic and aromatic amino acids were the preferred residues at the P1 position. For furylacryloyl peptides substrates, which are typical substrates for thermolysin, the enzyme exhibited high hydrolytic activity with a Km values of 0.858 and 2.363 mM for N-(3-[2-Furyl]acryloyl)-Ala-Phe amide and N-(3-[2-Furyl]acryloyl)-Gly-Leu amide, respectively. The purified protease hydrolysed proteins substrates such as azocasein, azocoll, keratin azure and wool.
Ključne besede:textile finishing, enzymatic modification, wool fibre, enzymes, Bacilus cereus, specificity, kinetics, metalloprotease
Leto izida:2007
UDK:677.31.027.6:577.15
COBISS_ID:11072278 Novo okno
ISSN pri članku:0141-0229
NUK URN:URN:SI:UM:DK:ASFWMV1C
Število ogledov:1419
Število prenosov:83
Metapodatki:XML RDF-CHPDL DC-XML DC-RDF
Področja:Ostalo
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Gradivo je del revije

Naslov:Enzyme and microbial technology
Skrajšan naslov:Enzyme microb. technol.
Založnik:Butterworth Scientific Ltd
ISSN:0141-0229
COBISS.SI-ID:25406976 Novo okno

Sekundarni jezik

Jezik:Angleški jezik
Ključne besede:plemenitenje tekstilij, enzimska modifikacija, volnena vlakna, encimi, Bacilus cereus, proteaze, specifičnost, kinetika, metaloproteaza


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