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Title:Rotaviral RNA found on various surfaces in a hospital laundry
Authors:Fijan, Sabina (Author)
Steyer, Andrej (Author)
Poljšak-Prijatelj, Mateja (Author)
Cencič, Avrelija (Author)
Šostar-Turk, Sonja (Author)
Koren, Srečko (Author)
Files:URL http://dx.doi.org/10.1016/j.jviromet.2007.10.011
Work type:Unknown ()
Typology:1.01 - Original Scientific Article
Organization:FS - Faculty of Mechanical Engineering
Abstract:The aim of this investigative study was to determine the presence of rotaviral RNA at various control points (CP) of a hospital laundry. One of the possible sources of hospital infections is inappropriately laundered and disinfected hospital textiles. RT-PCR and nested PCR for gene amplification using specific primers following RNA isolation were used to determine the presence of rotaviral RNA on swabs. In addition, rotavirus suspensions were inoculated on marked surfaces as positive controls for different surfaces (cotton textiles, folding table and industrial dryer). Rotaviral RNA was found on various laundry surfaces: technical equipment, storage shelves, transport vehicles, personnel's hands, damp textiles, and folded laundry. Rotaviral RNA was also detected at all positive controls on tested surfaces after 24 h. Based on the results, it is very important to take into consideration the proper handling of textiles after washing as one of the precautions against hospital-acquired infections. This paper reports the presence of rotaviral RNA for the first time on surfaces in laundries and equipment, as well as textiles.
Keywords:laundry hygiene, rotaviruses, hospital-acquired disease prevention, sanitary measures, occupational health
Year of publishing:2008
ISSN on article:0166-0934
COBISS_ID:11935766 Link is opened in a new window
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Record is a part of a journal

Title:Journal of virological methods
Shortened title:J. virol. methods
COBISS.SI-ID:25810432 New window

Secondary language

Keywords:higiena bolnišničnega perila, rota virus, preprečevanje bolnišničnih okužb, sanitarno-mikrobiološki parametri, delovna higiena


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