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Title:Supercritical fluids as solvents for enzymatic reactions
Authors:Leitgeb, Maja (Author)
Primožič, Mateja (Author)
Knez, Željko (Author)
Files:.pdf Acta_Chimica_Slovenica_2007_Habulin,_Primozic,_Knez_Supercritical_fluids_as_solvents_for_enzymatic_reactions.pdf (177,49 KB)
MD5: A5A4F4950D99DEAB2EAFEB295C089E7A
URL http://acta-arhiv.chem-soc.si/54/54-4-667.pdf
Work type:Scientific work (r2)
Typology:1.02 - Review Article
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Enzymes may act in different solvent systems. Water as the solvent in vivo may be replaced partially or mostly with other solvents, such as micro-emulsions, organic solvents, reversed micelles, ionic liquids and supercritical fluids (SCFs).Several types of enzymatic reactions were performed in SCFs. Influence of SCFs on enzyme stability and activity is presented on different examples; on different reaction systems (hydrolysis, transesterification...) and on the use of non-immobilized (Subtilisin carlsberg, Aspergillus niger...) as well as immobilized enzymes. Several types of high-pressure enzymatic reactors (batch-, stirred-tank-, extractive semibatch-, recirculating batch-, semicontinuous flow-, continuous packed-bed-, and continuous-membrane reactors) have been used for the performance of enzymatic reactions. In the studies on stability of biocatalysts in a high-pressure batch-stirred tank reactor changes in biocatalysts activity due to pressurization/depressurization steps were observed. Interesting alternative to overcome this inconvenience is the use of the high-pressure continuous membrane reactors, where just single compression and expansion step is necessary.
Keywords:enzymatic reactions, supercritical carbon dioxide, high-pressure, enzyme stability, high-pressure reactors
Year of publishing:2007
Number of pages:str. 667-677
Numbering:št. 4, Letn. 54
ISSN on article:1318-0207
COBISS_ID:12002582 New window
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Record is a part of a journal

Title:Acta Chimica Slovenica
Shortened title:Acta Chim. Slov.
Publisher:Slovensko kemijsko društvo
COBISS.SI-ID:14086149 New window


License:CC BY 4.0, Creative Commons Attribution 4.0 International
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:31.05.2012

Secondary language

Abstract:Encimi so lahko aktivni v različnih okoljih. Vodo, kot topilo in vivo, lahko delno ali v celoti nadomestimo z drugimi topili, kot so; mikro-emulzije, organska topila, reverzni miceliji, ionske tekočine in superkritični fluidi (SCFs). V SCFs so bile izvedene mnoge encimsko katalizirane reakcije. Vpliv SCF na stabilnost in aktivnost encima je v članku predstavljen na različnih primerih; z uporabo različnih reakcijskih sistemov (hidroliza, transesterifikacija ...), z uporabo prostih (Subtilisin carlsberg, Aspergillus niger …) in imobiliziranih encimov. Za izvedbo encimsko kataliziranih reakcij se uporabljajo naslednji tipi visokotlačnih encimskih reaktorjev; šaržni mešalni, ekstrakcijski polšaržni, recirkulacijski šaržni, polkontinuirani pretočni, kontinuirani z nasutim slojem biokatalizatorja in kontinuirani membranski reaktorji. Študije stabilnosti biokatalizatorja v visokotlačnem šaržnem mešalnem reaktorju so pokazale, da se aktivnost biokatalizatorja spreminja zaradi kompresije/dekompresije po vsaki šarži. Temu se lahko izognemo z uporabo visokotlačnega kontinuiranega membranskega reaktorja.
Keywords:encimske reakcije, superkritični $CO_2$, visoki tlaki, stabilnost encimov, visokotlačni reaktorji


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