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Title:IZDELAVA IN KARAKTERIZACIJA HITOZANSKIH NANODELCEV ZA MEDICINSKO UPORABO
Authors:Kolar, Metod (Author)
Leitgeb, Maja (Mentor) More about this mentor... New window
Srčič, Stanko (Co-mentor)
Fras Zemljič, Lidija (Co-mentor)
Files:.pdf UNI_Kolar_Metod_2010.pdf (3,22 MB)
MD5: 910F743495561CDC8969056263F691C1
 
Language:Slovenian
Work type:Undergraduate thesis (m5)
Typology:2.11 - Undergraduate Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Namen diplomskega dela je bil izdelati in okarakterizirati nanodelce, ki nastanejo s tehniko ionotropnega geliranja iz hitozana in natrijevega tripolifosfata (TPP). Nanodelcem smo določili naboj s polielektrolitsko titracijo, velikosti z metodo dinamičnega sipanja laserske svetlobe (DLS) in zeta potencial z elektroforetičnim sipanjem svetlobe ter protimikrobni učinek po standardu ASTM E 2149-01. Preverili smo vpliv različnih masnih razmerij reaktantov in vpliv dodatka stabilizatorja polietilen glikola (PEG) na lastnosti disperzije nanodelcev. Ugotovili smo, da se s povečanjem masnega razmerja hitozan/TPP povečujejo: množina prostih amino skupin in s tem naboj, hidrodinamski radij ter zeta potencial, kakor tudi protimikrobni učinek na nekatere testirane mikroorganizme. Ugotovili smo, da dodatek stabilizatorja polietilen glikola (PEG) k disperziji nanodelcev ne vpliva izrazito na spremembe hidrodinamskega premera nanodelcev, medtem ko znižuje heterodisperznost velikosti nanodelcev in zeta potencial, in tako naredi disperzije manj stabilne. Iz tega sklepamo, da PEG proučevanih molekulskih mas ni najustreznejši stabilizator za disperzije hitozanskih nanodelcev. Ugotovili smo, da so nanodelci protimikrobno učinkoviti; presenetljivo celo bolje inhibirajo rast mikroorganizmov kot sama raztopina hitozana. Nano-formulacija hitozana omogoča njegovo optimalno in nadzorovano difuzijo v celice patogenih mikroorganizomov in je tako njihova degradacije še učinkovitejša.
Keywords:hitozan, nanodelci, TPP, ionotropno geliranje, ionsko geliranje, protimikrobna učinkovitost
Year of publishing:2010
Publisher:[M. Kolar]
Source:Maribor
UDC:577.15:66.098:620.3(043.2)
COBISS_ID:14560278 New window
NUK URN:URN:SI:UM:DK:YRTTXPAX
Views:2972
Downloads:387
Metadata:XML RDF-CHPDL DC-XML DC-RDF
Categories:KTFMB - FKKT
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Secondary language

Language:English
Title:PRODUCTION AND CHARACTERIZATION OF CHITOSAN NANOPARTICLES FOR MEDICAL USE
Abstract:In this study, we tried to obtain nanoparticles from chitosan and sodium tripolyphosphate (TPP) using ionotropic gelation techniques. The obtained nanoparticles were characterized for positive charge using polyelectrolyte titrations, particle size using dynamic light scattering (DLS), zeta potential of particles using electrophoretic light scattering, as well as antibacterial activities of nanoparticles were examined using standard ASTM E 2149-01. The results show that an increase in the w/w ratio of chitosan/TPP triggers an increase in the amount of free amino groups (positive charge), hydrodynamic diameter, zeta potential and also intensifies the antimicrobial effect of nanoparticles. The results also show that the addition of stabilizer polyethylene glycol (PEG) does not have major effect on the hydrodynamic diameter of nanoparticles, however it decreases heterodispersity of the size as well zeta potential, thus making dispersions less stable. This leads to the conclusion that PEG is not an appropriate stabilizer for studied nanoparticles dispersions. Moreover, the antibacterial activity results suggests that the chitosan in nanoparticulate form display higher antibacterial activity against all tested microorganisms than the chitosan in the free form. Nano-formulation of chitosan enables optimal and controlled diffusion of chitosan into the cells of pathogen microorganisms, thus making their degradation more efficient.
Keywords:chitosan, nanoparticles, TPP, ionotropic gelation, ionic gelation, antimicrobial activity


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