1. Recent developments in electrochemical-impedimetric biosensors for virus detectionZala Štukovnik, Urban Bren, 2022, pregledni znanstveni članek Opis: Viruses, including influenza viruses, MERS-CoV (Middle East respiratory syndrome coronavirus), SARS-CoV (severe acute respiratory syndrome coronavirus), HAV (Hepatitis A virus), HBV (Hepatitis B virus), HCV (Hepatitis C virus), HIV (human immunodeficiency virus), EBOV (Ebola virus), ZIKV (Zika virus), and most recently SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2), are responsible for many diseases that result in hundreds of thousands of deaths yearly. The ongoing outbreak of the COVID-19 disease has raised a global concern and intensified research on the detection of viruses and virus-related diseases. Novel methods for the sensitive, rapid, and on-site detection of pathogens, such as the recent SARS-CoV-2, are critical for diagnosing and treating infectious diseases before they spread and affect human health worldwide. In this sense, electrochemical impedimetric biosensors could be applied for virus detection on a large scale. This review focuses on the recent developments in electrochemical-impedimetric biosensors for the detection of viruses.
Ključne besede: electrochemical impedance spectroscopy, impedimetric biosensor, genosensor, aptasensor, immunosensor, virus detection, SARS-CoV-2, HIV, influenza virus, hepatitis virus
Objavljeno v DKUM: 05.12.2024; Ogledov: 0; Prenosov: 7
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2. Covalent modification of chitosan surfaces with a sugar amino acid and lysine analoguesTobias Dorn, Matjaž Finšgar, Karin Stana-Kleinschek, Tobias Alexander Steindorfer, Martin Simon Thonhofer, Tanja Wrodnigg, Rupert Kargl, 2024, izvirni znanstveni članek Opis: This work explores the modifcation and characterization of chitosan thin flms as a model for functionalized polysaccharide
interfaces. The solid–liquid interface of oligo- and polysaccharides is crucial for various biological processes such as cell
adhesion and recognition. By covalent surface modifcation of the chitosan via amide formation with diferent small molecules containing carboxylic acids, e.g. specially designed glycoside hydrolase inhibitors, interactions with biomolecules and
living cells could potentially be controlled in the future. As a frst step towards this aim, three fuorescent compounds were
conjugated onto nanometric chitosan thin flms. The layers were analysed by fuorescence spectroscopy, X-ray photoelectron
spectroscopy, time-of-fight secondary ion mass spectrometry, and atomic force microscopy, to proof the covalent attachment
of the target molecules. By this analysis, a uniform and chemically stable covalent attachment of the target molecules on
the chitosan thin flms could be demonstrated under various conditions. This publication serves as a proof-of-concept-study
for further biofunctionalization, pattering, and interaction studies involving polysaccharide interfaces, glycosidase inhibitors, proteins, or living cells.
Ključne besede: carbohydrates, fluorescence spectroscopy, amino acid, chitosan, thin flm modifcation, C-Glycosides
Objavljeno v DKUM: 07.08.2024; Ogledov: 127; Prenosov: 12
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3. Properties of the fluoroacrylate and methacryloxypropyl-trimethoxysilane applied to a layer of Cu2O on bronze as either single or multi-component coatingsLuka Škrlep, Tadeja Kosec, Matjaž Finšgar, Andrijana Sever Škapin, Erika Švara Fabjan, 2023, izvirni znanstveni članek Ključne besede: bronze, Cu2O layer on bronze, fluoropolymer coating, protection efficiency, surface spectroscopy
Objavljeno v DKUM: 10.05.2024; Ogledov: 183; Prenosov: 12
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4. Influence of supercritical carbon dioxide on the activity and conformational changes of α-amylase, lipase, and peroxidase in the solid state using white wheat flour as an exampleMilena Ivanović, Željko Knez, Maja Leitgeb, 2023, izvirni znanstveni članek Ključne besede: enzyme inactivation, supercritical carbon dioxide, a-amilaze, lipase, antioxidants, horseradish peroxidase, structural changes, CD spectroscopy
Objavljeno v DKUM: 22.04.2024; Ogledov: 183; Prenosov: 12
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5. Review of bioplastics characterisation by terahertz techniques in the view of ensuring a circular economyAndreja Abina, Tjaša Korošec, Uroš Puc, Aleksander Zidanšek, 2023, pregledni znanstveni članek Opis: The increasing scarcity of natural resources, worsening global climate change, environmental degradation, and rising demand for food are forcing the biotechnology and plastics industries to seek and apply circular economy models that would lead to a sustainable transition in the production and use of bioplastics. Circular economy models can improve the economic productivity of bio-based plastics and have a positive impact on the environment by reducing conventional plastic waste and the consumption of petrochemical feedstocks for plastic production. In addition, some agricultural wastes that have the potential to be used as bioplastics can be reused. Terahertz (THz) systems are already used in the plastics and rubber industries for non-destructive testing, detection, imaging, and quality control. Several reports have highlighted the potential applications of THz spectroscopy and imaging in polymer analysis and plastics characterisation. This potential is even greater with chemometric methods and artificial intelligence algorithms. In this review, we focus on applications that support the transformation of the biotechnology sector to the circular economy, particularly via the transition from conventional plastics to bioplastics. In this review, we discuss the potential of THz systems for the characterisation and analysis of bioplastics and biopolymers. The results of previous studies on biopolymers in the THz frequency range are summarised. Furthermore, the potential of using artificial intelligence approaches such as machine learning as advanced analytical methods in THz spectroscopy and imaging, in addition to the conventionally used chemometric methods, is discussed. The results of this review highlight that THz technology can contribute to closed technological circles in important areas of biotechnology and the related plastics and rubber industries.
Ključne besede: terahertz spectroscopy, terahertz imaging, circular economy, biopolymer, biotechnology, bioplastics
Objavljeno v DKUM: 16.04.2024; Ogledov: 354; Prenosov: 189
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6. Insights into the stabilization of interferon alpha by two surfactants revealed by STD-NMR spectroscopyHristo Svilenov, Katharina T. Kopp, Alexander P. Golovanov, Gerhard Winter, Matja Zalar, 2023, drugi znanstveni članki Ključne besede: drug-excipient interactions, protein aggregation, protein formulations, proteins, surfactants, Nuclear Magnetic Resonance, NMR, spectroscopy
Objavljeno v DKUM: 12.04.2024; Ogledov: 197; Prenosov: 19
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7. Analytical gas sensing in the terahertz spectral rangeAndreja Abina, Uroš Puc, Mojca Jazbinšek, Aleksander Zidanšek, 2023, izvirni znanstveni članek Opis: Exploiting the terahertz (THz) part of the electromagnetic spectrum is attracting attention in various scientific and applied disciplines worldwide. THz technology has also revealed its potential as an effective tool for gas analysis in astronomy, biomedicine and chemical analysis. Recently, it has also become important in environmental applications for monitoring hazardous and toxic gases in the atmosphere. This paper gives an overview of THz gas detection analytical methods for environmental and biomedical applications, starting with a brief introduction to THz technology and an explanation of the interaction of THz radiation with gaseous species and the atmosphere. The review focuses on several gaseous species and groups of air pollutants that have been or can be analysed by THz spectrometry. The review concludes that different but complementary THz detection methods allow unique detection, identification and quantification of gaseous and particulate air pollutants with high selectivity, specificity and sensitivity. THz detection methods also allow further technological improvements and open new application possibilities.
Ključne besede: terahertz spectroscopy, gas sensing, air pollution, environmental monitoring
Objavljeno v DKUM: 28.03.2024; Ogledov: 205; Prenosov: 18
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8. Urinary metabolic biomarker profiling for cancer diagnosis by terahertz spectroscopy : review and perspectiveAndreja Abina, Tjaša Korošec, Uroš Puc, Mojca Jazbinšek, Aleksander Zidanšek, 2023, pregledni znanstveni članek Opis: In the last decade, terahertz (THz) technologies have been introduced to the detection, identification, and quantification of biomolecules in various biological samples. This review focuses on substances that represent important biomarkers in the urine associated with various cancers and their treatments. From a diagnostic point of view, urine liquid biopsy is particularly important because it allows the non-invasive and rapid collection of large volumes of samples. In this review, the THz spectral responses of substances considered metabolic biomarkers in urine and obtained in previous studies are collected. In addition, the findings from the relatively small number of prior studies that have already been carried out on urine samples are summarised. In this context, we also present the different THz methods used for urine analysis. Finally, a brief discussion is given, presenting perspectives for future research in this field, interpreted based on the results of previous studies. This work provides important information on the further application of THz techniques in biomedicine for detecting and monitoring urinary biomarkers for various diseases, including cancer.
Ključne besede: terahertz spectroscopy, urinary biomarkers, metabolic biomarkers, cancer diagnostics, biomolecules, non-invasive detection, biomedical detection
Objavljeno v DKUM: 14.03.2024; Ogledov: 212; Prenosov: 32
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9. Nanomaterials and their recent applications in impedimetric biosensingZala Štukovnik, Regina Fuchs-Godec, Urban Bren, 2023, pregledni znanstveni članek Opis: Impedimetric biosensors measure changes in the electrical impedance due to a biochemical process, typically the binding of a biomolecule to a bioreceptor on the sensor surface. Nanomaterials can be employed to modify the biosensor's surface to increase the surface area available for biorecognition events, thereby improving the sensitivity and detection limits of the biosensor. Various nanomaterials, such as carbon nanotubes, carbon nanofibers, quantum dots, metal nanoparticles, and graphene oxide nanoparticles, have been investigated for impedimetric biosensors. These nanomaterials have yielded promising results in improving sensitivity, selectivity, and overall biosensor performance. Hence, they offer a wide range of possibilities for developing advanced biosensing platforms that can be employed in various fields, including healthcare, environmental monitoring, and food safety. This review focuses on the recent developments in nanoparticle-functionalized electrochemical-impedimetric biosensors.
Ključne besede: impedimetric biosensor, electrochemical impedance spectroscopy, nanomaterials, metal nanoparticles, carbon nanofibers, carbon nanotubes, graphene oxide, quantum dots
Objavljeno v DKUM: 12.02.2024; Ogledov: 6175; Prenosov: 24
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10. Frequency range optimization for continuous wave Terahertz imagingBlaž Pongrac, Andrej Sarjaš, Dušan Gleich, izvirni znanstveni članek Opis: S krajšimi valovnimi dolžinami kot mikrovalovi in večjo globino prodora v material kot infrardeča svetloba, valovi v TeraHertz-nem (THz) spektru ponujajo edinstvene možnosti testiranja materialov. THz tehnologija ponuja neinvazivna in nedestruktivna testiranja v obliki spektroskopije in slikanja. Najbolj uporabljeni sistemi za THz slikanje so sistemi spektroskopije v časovni domeni. Vendar sistemi spektroskopije frekvenčne domene ponujajo odlično frekvenčno ločljivost in so primerni za biomedicinske aplikacije. THz-no slikanje na podlagi spektroskopije v frekvenčnem prostoru je časovno kompleksno in ima pomanjkljivosti zaradi napak pri generiranju THz valov. V tem članku je predstavljen nov princip enodimenzionalnega zajemanja s THz valovi. Predlagana optimizacija frekvenčnega območja temelji na konvolucijski nevronski omreži. Predstavljena je frekvenčna optimizacija za določitev optimalnega frekvenčnega območja za zajem podatkov. Optimalno frekvenčno območje ali pasovna širina morata biti dovolj široka za učinkovito zaznavanje faze in morata biti na presečišču več spektralnih odtisov v opazovanem mediju. Presek spektralnih odtisov je ocenjen z uporabo predlaganega algoritma za optimizacijo frekvenčnega območja, ki temelji na konvolucijski nevronski mreži in algoritmu za občutljivost okluzije. Predlagani algoritem izbira samodejno najobčutljivejši frekvenčni pas THz spektra in omogoča zelo hitre zajeme za pregled in klasifikacijo objektov.
Ključne besede: terahertz, spectroscopy, imaging, convolutional neural network, occlusion sensitivity, optimization
Objavljeno v DKUM: 07.12.2023; Ogledov: 421; Prenosov: 23
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