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In vitro tissue culture initiation from potted and garden Hydrangea macrophylla explants
Metka Šiško, 2016, original scientific article

Abstract: The effect of sterilisation treatments on the initiation of the culture of three Hydrangea macrophylla genotypes in vitro was studied. The results indicated difference among different disinfection treatment; the consequence of treatments with dichloroisocyanuric acid, in combination with silver nitrate, was a significantly higher survival rate comparing to treatments based on NaOCl. Success of sterilisation was significantly influenced by studied genotypes. Two potted hydrangea genotypes (G2 and G3) showed higher survival rate (45.00 %, 55.00 %, respectively) comparing to the genotype 1 (originated from a local garden), which exhibited the highest percentage of contamination rate (58.75 %). Culturing the explants on a McCown woody plant medium supplemented with BAP at 2 mg/L and NAA at 0.005 mg/L produced the highest number of shoots with multiplication rate of 1.31 in 5 weeks. The highest rooting percentage (100.00 %) was obtained with the genotype 2 on a McCown woody plant medium supplemented with 0.5 mg/L IBA.
Keywords: micropropagation, tissue culture, hydrangea, Hydrangea macrophylla, plant disinfection
Published: 14.11.2017; Views: 883; Downloads: 264
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Biomaterials and host versus graft response
Tomaž Velnar, Gorazd Bunc, Robert Klobucar, Lidija Gradišnik, 2016, review article

Abstract: Biomaterials and biotechnology are increasing becoming an important area in modern medicine. The main aim in this area is the development of materials, which are biocompatible to normal tissue. Tissue-implant interactions with molecular, biological and cellular characteristics at the implant-tissue interface are important for the use and development of implants. Implantation may cause an inflammatory and immune response in tissue, foreign body reaction, systemic toxicity and imminent infection. Tissue-implant interactions determine the implant life-period. The aims of the study are to consider the biological response to implants. Biomaterials and host reactions to implants and their mechanisms are also briefly discussed.
Keywords: host versus graft disease, GVHD, biomaterial, wound healing, transplant, tissue, prosthetic, implants, biological response, complications
Published: 03.08.2017; Views: 679; Downloads: 102
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Micropropagation of the narrow endemic Hladnikia pastinacifolia (Apiaceae)
Jana Ambrožič-Dolinšek, Terezija Ciringer, Mitja Kaligarič, 2016, original scientific article

Abstract: The monotypic Hladnikia pastinacifolia Rchb. is a narrow endemic species, with an extremely small distribution area in Slovenia, prone to any kind of threat that could lead to species extinction. Tissue culture techniques are proposed as a conservation measure for rapid propagation and ex-situ conservation. Tissue culture was initiated from seeds and juvenile plants obtained from natural sites on a solid Murashige and Skoog (MS) medium, with and without growth regulators. We tested various combinations and concentrations of growth regulators, and the best proliferation of axillary shoots, on average 14, was obtained on MS medium with 5 μM BAP and 3 μM IBA and 3% sucrose. Rooting was achieved after transferral of the shoots to an MS medium with 2 μM IBA and 3% sucrose. The rooted plants were acclimatized on a mixture of limestone sand, potting soil and vermiculite in a ratio of 10:2:2, with pH in the range of 7.5–8.0. In vitro propagation methods provide an important opportunity for the propagation and preservation of H. pastinacifolia by rapidly increasing the number of plants, without disturbing the wild population.
Keywords: biotechnology, micropropagation, micropropagation, tissue culture, Hladnikia pastinacifolia, Apiaceae
Published: 28.03.2017; Views: 895; Downloads: 329
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Tissue culture of Pyrethrum (Tanacetum cinerariifolium) and associated microbial contamination
Jana Ambrožič-Dolinšek, Maja Ravnikar, Jana Žel, Tina Demšar, Marjana Camloh, Katarina Cankar, Tanja Dreo, 2010, original scientific article

Abstract: Microbial contamination was observed on several subcultures of Pyrethrum (Tanacetum cinerariifolium) (Trevir.) Schultz-bip. callus lines. The presence of microorganisms was detected by isolation of contaminants in pure culture from 7 out of 34 callus lines and direct amplification of eubacterial 16S rDNA in the pyrethrum callus and plants and isolated bacteria. Altogether 16 contaminants were further analyzed, observing their morphology on several media and restriction of amplified 16S rDNA. Analysis revealed presence and persistence of morphologically and genetically diverse bacteria in pyrethrum tissue culture. Due to cross reactivity of 16S rDNA primers with DNA of plant origin, no conclusions could be drawn on the origin of contaminants.
Keywords: tissue cultures, Pyrethrum, Tanacetum cinerariifolium, callus culture, microbial contamination, PCR
Published: 21.12.2015; Views: 851; Downloads: 18
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Is tissue augmentation a reality in biosurgery? An experimental study of endothelial cell invasion into tissue filler
Tomaž Velnar, Vladimir Smrkolj, Marjan Rupnik, Lidija Gradišnik, 2013, original scientific article

Abstract: New therapeutic approaches for wound treatment are evolving. Non healing wounds in oncology and after trauma may be cured by a novel technique of tissue augmentation with soft tissue fillers. The principle resides in filling the wound with collagen filler in order to seal the defect and promote healing. Successful angiogenesis forms the basis of tissue filler survival and determines the outcome of the healing process. During this study, basic data about endothelial cell invasion into collagen-made substratum was collected that could be used for neoangiogenesis studies in tissue augmentation techniques for large wound defect treatment. In the in vitro assay, the human umbilical vein endothelial cells (HUVEC) grow into a three-dimensional framework of collagenous tissue fillers, forming the basic step for angiogenesis. After heparins were used as chemotactic agents, a typical bell-shaped relationship between chemotaxis and agent concentrations was found. Significant cell infiltration was present in the assays with chemotactic agents. These observations support the potential for tissue augmentation with soft tissue fillers that could be used in acute and chronic non healing traumatic and oncology wounds after extensive surgical resections and radiotherapy.
Keywords: angiogenesis, cell invasion, tissue augmentation, tissue filler, wound healing
Published: 10.07.2015; Views: 881; Downloads: 79
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