| | SLO | ENG | Piškotki in zasebnost

Večja pisava | Manjša pisava

Iskanje po katalogu digitalne knjižnice Pomoč

Iskalni niz: išči po
išči po
išči po
išči po
* po starem in bolonjskem študiju

Opcije:
  Ponastavi


1 - 8 / 8
Na začetekNa prejšnjo stran1Na naslednjo stranNa konec
1.
Theoretical and experimental investigation of calcium-contraction coupling in airway smooth muscle
Prisca Mbikou, Aleš Fajmut, Milan Brumen, Etienne Roux, 2006, izvirni znanstveni članek

Opis: We investigated theoretically and experimentally the ▫$Ca^{2+}$▫-contraction couplingin rat tracheal smooth muscle. ▫$[Ca^{2+}]_i$▫, isometric contraction and myosin light chain (MLC) phosphorylation were measured in response to 1 mM carbachol. Theoretical modeling consisted in coupling a model of ▫$Ca^{2+}-dependent$▫ MLC kinase (MLCK) activation with a four-state model of smooth muscle contractile apparatus. Stimulation resulted in a short-time contraction obtained within 1 min, followed by a long-time contraction up to the maximal force obtained in 30 min. ML-7 and Wortmannin (MLCK inhibitors) abolished the contraction. Chelerythrine (PKC inhibitor) did not change the short-time, but reduced the long-time contraction. ▫$[Ca^{2+}]_i$▫ responses of isolated myocytes recorded during the first 90 s consisted in a fast peak, followed by a plateau phase and, in 28 % of the cells, superimposed ▫$Ca^{2+}$▫ oscillations. MLC phosphorylation was maximal at 5 s and then decreased, whereas isometric contraction followed a Hill-shaped curve. The model properlypredicts the time course of MLC phosphorylation and force of the short-time response. With oscillating ▫$Ca^{2+}$▫ signal, the predicted force does not oscillate. According to the model, the amplitude of the plateau and the frequency of oscillations encode for the amplitude of force, whereas the peak encodes for force velocity. The long-time phase of the contraction, associated with a second increase in MLC phosphorylation, may be explained, at least partially, by MLC phosphatase (MLCP) inhibition, possibly via PKC inhibition.
Ključne besede: biophysics, mathematical modelling, modelling, calcium oscillations, contractions, force development, muscle cells, smooth muscles, myosin kinase
Objavljeno: 07.06.2012; Ogledov: 935; Prenosov: 52
URL Povezava na celotno besedilo

2.
Modeling of molecular and cellular mechanisms involved in [Ca sup 2+] signal encoding in airway myocytes
Marko Marhl, Denis Noble, Etienne Roux, 2006, pregledni znanstveni članek

Opis: In airway myocytes signal transduction via cytosolic calcium plays an important role. In relation with experimental results we review models of basic molecular and cellular mechanisms involved in the signal transduction from the myocyte stimulation to the activation of the contractile apparatus. We concentrate on mechanisms for encoding of input signals into Ca2+ signals and the mechanisms for their decoding. The mechanisms are arranged into a general scheme of cellular signaling, the so-called bow-tie architecture of signaling, in which calcium plays the role of a common media for cellular signals and links the encoding and decoding part. The encoding of calcium signals in airway myocytes is better known and is presented in more detail. Inparticular, we focus on three recent models taking into account the intracellular calcium handling and ion fluxes through the plasma membrane. Themodel of membrane conductances was originally proposed for predicting membrane depolarization and voltage-dependent Ca2+ influx triggered by initialcytosolic Ca2+ increase as observed on cholinergic stimulation. Cellular models of intracellular Ca2+ handling were developed to investigate the role of a mixed population of InsP3 receptor isoforms and the cellular environment in the occurrence of Ca2+ oscillations, and the respective role ofthe sarcoplasmic reticulum, mitochondria, and cytosolic Ca2+-binding proteins in cytosolic Ca2+ clearance. Modeling the mechanisms responsible for the decoding of calcium signals is developed in a lesser extent; however, the most recent theoretical studies are briefly presented in relation with the known experimental results.
Ključne besede: biophysics, mathematical modelling, modelling, calcium oscillations, contractions, airway smooth muscle cells, muscle cells, smooth muscles, encoding, decoding, bow-tie structures
Objavljeno: 07.06.2012; Ogledov: 1047; Prenosov: 13
URL Povezava na celotno besedilo

3.
The extraction of neural information from the surface EMG for the control of upper-limb prostheses
Dario Farina, Ning Jiang, Hubertus Rehbaum, Aleš Holobar, Bernhard Graimann, Hans Dietl, Oskar Aszmann, 2014, izvirni znanstveni članek

Opis: Despite not recording directly from neural cells, the surface electromyogram (EMG) signal contains information on the neural drive to muscles, i.e., the spike trains of motor neurons. Using this property, myoelectric control consists of the recording of EMG signals for extracting control signals to command external devices, such as hand prostheses. In commercial control systems, the intensity of muscle activity is extracted from the EMG and used for single degrees of freedom activation (direct control). Over the past 60 years, academic research has progressed to more sophisticated approaches but, surprisingly, none of these academic achievements has been implemented in commercial systems so far. We provide an overview of both commercial and academic myoelectric control systems and we analyze their performance with respect to the characteristics of the ideal myocontroller. Classic and relatively novel academic methods are described, including techniques for simultaneous and proportional control of multiple degrees of freedom and the use of individual motor neuron spike trains for direct control. The conclusion is that the gap between industry and academia is due to the relatively small functional improvement in daily situations that academic systems offer, despite the promising laboratory results, at the expense of a substantial reduction in robustness. None of the systems so far proposed in the literature fulfills all the important criteria needed for widespread acceptance by the patients, i.e. intuitive, closed-loop, adaptive, and robust real-time ( 200 ms delay) control, minimal number of recording electrodes with low sensitivity to repositioning, minimal training, limited complexity and low consumption. Nonetheless, in recent years, important efforts have been invested in matching these criteria, with relevant steps forwards.
Ključne besede: neural drive to muscle, high-density EMG, motor neuron, motor unit, myoelectronic control, pattern recognition, regression
Objavljeno: 25.05.2015; Ogledov: 492; Prenosov: 0

4.
Noninvasive, accurate assessment of the behavior of representative populations of motor units in targeted reinnervated muscles
Dario Farina, Hubertus Rehbaum, Aleš Holobar, Ivan Vujaklija, Ning Jiang, Christian Hofer, Stefan Salminger, Hans-Willem van Vliet, Oskar Aszmann, 2014, izvirni znanstveni članek

Opis: Targeted muscle reinnervation (TMR) redirects nerves that have lost their target, due to amputation, to remaining muscles in the region of the stump with the intent of establishing intuitive myosignals to control a complex prosthetic device. In order to directly recover the neural code underlying an attempted limb movement, in this paper, we present the decomposition of high-density surface electromyographic (EMG) signals detected from three TMR patients into the individual motor unit spike trains. The aim was to prove, for the first time, the feasibility of decoding the neural drive that would reach muscles of the missing limb in TMR patients, to show the accuracy of the decoding, and to demonstrate the representativeness of the pool of extracted motor units. Six to seven flexible EMG electrode grids of 64 electrodes each were mounted over the reinnervated muscles of each patient, resulting in up to 448 EMG signals. The subjects were asked to attempt elbow extension and flexion, hand open and close, wrist extension and flexion, wrist pronation and supination, of their missing limb. The EMG signals were decomposed using the Convolution Kernel Compensation technique and the decomposition accuracy was evaluated with a signal-based index of accuracy, called pulse-to-noise ratio (PNR). The results showed that the spike trains of 3 to 27 motor units could be identified for each task, with a sensitivity of the decomposition > 90%, as revealed by PNR. The motor unit discharge rates were within physiological values of normally innervated muscles. Moreover, the detected motor units showed a high degree of common drive so that the set of extracted units per task was representative of the behavior of the population of active units. The results open a path for a new generation of human-machine interfaces in which the control signals are extracted from noninvasive recordings and the obtained neural information is based directly on the spike trains of motor neurons.
Ključne besede: electromyographic, EMG, decomposition, high-density EMG, motor neuron, motor unit, myoelectronic control, neural drive to muscle, target muscle reinervation, TMR
Objavljeno: 25.05.2015; Ogledov: 593; Prenosov: 0

5.
Skeletal muscle derived cell cultures as potent models in regenerative medicine research
Robi Kelc, Martin Trapečar, Matjaž Vogrin, Avrelija Cencič, 2013, pregledni znanstveni članek

Opis: Cell cultures have been used extensively by many scientists in the last decades to study various cell and tissue mechanisms. They have many advantagesover in vivo experimental models, but their limitations are to be considered as well. As skeletal muscle-derived cell cultures are becoming increasingly interesting in studies of muscle regeneration processes the question of their relevance in experiments arises according to in vivo experimental models. This article reviews studies that have simultaneously performed in vivo and in vitro experiments on skeletal muscle and discusses the correlation of both results. Although they seem to correlate, no such studies on humans have been performed so far.
Ključne besede: skeletal muscle cell, cell culture, In vivo, In vitro, experimental model
Objavljeno: 10.07.2015; Ogledov: 611; Prenosov: 72
URL Povezava na celotno besedilo

6.
Progressive fastICA peel-off and convolution kernel compensation demonstrate high agreement for high density surface EMG decomposition
Maoqi Chen, Aleš Holobar, Xu Zhang, Ping Zhou, 2016, izvirni znanstveni članek

Opis: Decomposition of electromyograms (EMG) is a key approach to investigating motor unit plasticity. Various signal processing techniques have been developed for high density surface EMG decomposition, among which the convolution kernel compensation (CKC) has achieved high decomposition yield with extensive validation. Very recently, a progressive FastICA peel-off (PFP) framework has also been developed for high density surface EMG decomposition. In this study, the CKC and PFP methods were independently applied to decompose the same sets of high density surface EMG signals. Across 91 trials of 64-channel surface EMG signals recorded from the first dorsal interosseous (FDI) muscle of 9 neurologically intact subjects, there were a total of 1477 motor units identified from the two methods, including 969 common motor units. On average, 10.6 ± 4.3 common motor units were identified from each trial, which showed a very high matching rate of 97.85 ± 1.85% in their discharge instants. The high degree of agreement of common motor units from the CKC and the PFP processing provides supportive evidence of the decomposition accuracy for both methods. The different motor units obtained from each method also suggest that combination of the two methods may have the potential to further increase the decomposition yield.
Ključne besede: EMG, electromyograms, muscle, convultions
Objavljeno: 15.06.2017; Ogledov: 399; Prenosov: 186
.pdf Celotno besedilo (1,18 MB)
Gradivo ima več datotek! Več...

7.
Motor unit characteristics after targeted muscle reinnervation
Tamás Kapelner, Ning Jiang, Aleš Holobar, Ivan Vujaklija, Aidan Roche, Dario Farina, Oskar Aszmann, 2016, izvirni znanstveni članek

Opis: Targeted muscle reinnervation (TMR) is a surgical procedure used to redirect nerves originally controlling muscles of the amputated limb into remaining muscles above the amputation, to treat phantom limb pain and facilitate prosthetic control. While this procedure effectively establishes robust prosthetic control, there is little knowledge on the behavior and characteristics of the reinnervated motor units. In this study we compared the m. pectoralis of five TMR patients to nine able-bodied controls with respect to motor unit action potential (MUAP) characteristics. We recorded and decomposed high-density surface EMG signals into individual spike trains of motor unit action potentials. In the TMR patients the MUAP surface area normalized to the electrode grid surface (0.25 ± 0.17 and 0.81 ± 0.46, p < 0.001) and the MUAP duration (10.92 ± 3.89 ms and 14.03 ± 3.91 ms, p < 0.01) were smaller for the TMR group than for the controls. The mean MUAP amplitude (0.19 ± 0.11 mV and 0.14 ± 0.06 mV, p = 0.07) was not significantly different between the two groups. Finally, we observed that MUAP surface representation in TMR generally overlapped, and the surface occupied by motor units corresponding to only one motor task was on average smaller than 12% of the electrode surface. These results suggest that smaller MUAP surface areas in TMR patients do not necessarily facilitate prosthetic control due to a high degree of overlap between these areas, and a neural information—based control could lead to improved performance. Based on the results we also infer that the size of the motor units after reinnervation is influenced by the size of the innervating motor neuron.
Ključne besede: target muscle reinnervation, motor unit, controlling muscles
Objavljeno: 19.06.2017; Ogledov: 371; Prenosov: 200
.pdf Celotno besedilo (1,51 MB)
Gradivo ima več datotek! Več...

8.
Biomechanical characteristics of skeletal muscles and associations between running speed and contraction time in 8- to 13-year-old children
Jernej Završnik, Rado Pišot, Boštjan Šimunič, Peter Kokol, Helena Blažun, 2017, izvirni znanstveni članek

Opis: Objective: To investigate associations between running speeds and contraction times in 8- to 13-year-old children. Method: This longitudinal study analyzed tensiomyographic measurements of vastus lateralis and biceps femoris muscles’ contraction times and maximum running speeds in 107 children (53 boys, 54 girls). Data were evaluated using multiple correspondence analysis. Results: A gender difference existed between the vastus lateralis contraction times and running speeds. The running speed was less dependent on vastus lateralis contraction times in boys than in girls. Analysis of biceps femoris contraction times and running speeds revealed that running speeds of boys were much more structurally associated with contraction times than those of girls, for whom the association seemed chaotic. Conclusion: Joint category plots showed that contraction times of biceps femoris were associated much more closely with running speed than those of the vastus lateralis muscle. These results provide insight into a new dimension of children’s development.
Ključne besede: children, tensiomyography, running speed, contraction time, skelet muscle, motor development
Objavljeno: 13.07.2017; Ogledov: 413; Prenosov: 214
.pdf Celotno besedilo (454,48 KB)
Gradivo ima več datotek! Več...

Iskanje izvedeno v 0.1 sek.
Na vrh
Logotipi partnerjev Univerza v Mariboru Univerza v Ljubljani Univerza na Primorskem Univerza v Novi Gorici