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1.
In vitro tissue culture initiation from potted and garden Hydrangea macrophylla explants
Metka Šiško, 2016, original scientific article

Abstract: The effect of sterilisation treatments on the initiation of the culture of three Hydrangea macrophylla genotypes in vitro was studied. The results indicated difference among different disinfection treatment; the consequence of treatments with dichloroisocyanuric acid, in combination with silver nitrate, was a significantly higher survival rate comparing to treatments based on NaOCl. Success of sterilisation was significantly influenced by studied genotypes. Two potted hydrangea genotypes (G2 and G3) showed higher survival rate (45.00 %, 55.00 %, respectively) comparing to the genotype 1 (originated from a local garden), which exhibited the highest percentage of contamination rate (58.75 %). Culturing the explants on a McCown woody plant medium supplemented with BAP at 2 mg/L and NAA at 0.005 mg/L produced the highest number of shoots with multiplication rate of 1.31 in 5 weeks. The highest rooting percentage (100.00 %) was obtained with the genotype 2 on a McCown woody plant medium supplemented with 0.5 mg/L IBA.
Keywords: micropropagation, tissue culture, hydrangea, Hydrangea macrophylla, plant disinfection
Published in DKUM: 14.11.2017; Views: 1134; Downloads: 281
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2.
Micropropagation of the narrow endemic Hladnikia pastinacifolia (Apiaceae)
Jana Ambrožič-Dolinšek, Terezija Ciringer, Mitja Kaligarič, 2016, original scientific article

Abstract: The monotypic Hladnikia pastinacifolia Rchb. is a narrow endemic species, with an extremely small distribution area in Slovenia, prone to any kind of threat that could lead to species extinction. Tissue culture techniques are proposed as a conservation measure for rapid propagation and ex-situ conservation. Tissue culture was initiated from seeds and juvenile plants obtained from natural sites on a solid Murashige and Skoog (MS) medium, with and without growth regulators. We tested various combinations and concentrations of growth regulators, and the best proliferation of axillary shoots, on average 14, was obtained on MS medium with 5 μM BAP and 3 μM IBA and 3% sucrose. Rooting was achieved after transferral of the shoots to an MS medium with 2 μM IBA and 3% sucrose. The rooted plants were acclimatized on a mixture of limestone sand, potting soil and vermiculite in a ratio of 10:2:2, with pH in the range of 7.5–8.0. In vitro propagation methods provide an important opportunity for the propagation and preservation of H. pastinacifolia by rapidly increasing the number of plants, without disturbing the wild population.
Keywords: biotechnology, micropropagation, micropropagation, tissue culture, Hladnikia pastinacifolia, Apiaceae
Published in DKUM: 28.03.2017; Views: 1133; Downloads: 385
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3.
In vitro propagation of Gisela 5 (Prunus cerasus x P. canescens)
Metka Šiško, 2011, original scientific article

Keywords: Gisela 5, cherry rootstock, micropropagation, in vitro propagation
Published in DKUM: 10.07.2015; Views: 903; Downloads: 49
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