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1.
Hygiene monitoring systems for hospital textile laundering
Sabina Fijan, Sonja Šostar-Turk, 2007, strokovni članek

Opis: The main aim of washing laundry is to remove soils and microorganisms from infected and dirty textiles and achieve clean, fresh and disinfected textiles ready for use. Textiles undergo laundering processes, which include: soil removal with special laundering agents; bleaching; disinfecting; and finally neutralising and rinsing. Because textiles from hospitals may contain many kinds of pathogenic -bacteria, fungi and viruses, it is essential that the laundering process has not only a cleaning effect but also an antimicrobial one. Since users of hospital textiles are often patients with a weakened immune -system, it is recommended that best practice and common sense be employed when washing and -disinfecting hospital textiles. Most people assume that the laundry returned to them is in fact clean and, therefore, safe. Experience encourages all infection control teams to take laundering very seriously. (1-15) Inappropriately disinfected textiles are one of the possible sources of nosocomial infections for patients. There are reports of hospital textiles being the source of nosocomial infection with streptococci, enterococci, Bacillus cereus, staphylococci and coliforms.(5-9) There are some documented cases where staff in hospital wards and laundries have been infected with scabies, fungi, salmonellas, gastroenteritis viruses, hepatitis A viruses and coxiellas after treating dirty laundry.
Ključne besede: textile care, hospital laundry, laundry hygiene, micro-organisms, washing quality parameters, sanitary microbiological parameters, hospital-acquired disease prevention, sanitary measures
Objavljeno: 31.05.2012; Ogledov: 1789; Prenosov: 18
URL Povezava na celotno besedilo

2.
Implementing hygiene monitoring systems in hospital laundries in order to reduce microbial contamination of hospital textiles
Sabina Fijan, Sonja Šostar-Turk, Avrelija Cencič, 2005, izvirni znanstveni članek

Opis: As textiles sent to hospital laundries contain many types of pathogenic organisms, it is important that laundering not only has an appropriate cleaning effect but also has a satisfactory disinfecting effect. Critical to this process is the maintenance of an appropriate hygiene level in the clean area of laundries in order to prevent recontamination of textiles from manual handling when ironing, folding, packing etc. The aims of this study were to evaluate the hygienic state of a hospital laundry, to introduce continuous sanitary measures, and to introduce a continuous hygiene monitoring system with an infection control programme. Two systems for evaluating hospital laundry hygiene were combined: HACCP principles (hazard analysis and critical control points) and RAL-GZ 992 standards (quality assurance standard for textile care of hospital laundry). Evaluation of the hygienic state of the hospital laundry was carried out by evaluating the number and types of micro-organisms present at the critical control points throughout the whole laundering process, using RODAC agar plates for surface sampling and the pour plate method for investigating water samples. The initial examination showed that the sanitary condition of the laundry did not reach the required hygiene level. Therefore, fundamental sanitation measures were instituted and the examination was repeated. Results were then satisfactory. The most important critical control point was the chemothermal laundering efficiency of the laundering process. To prevent micro-organisms spreading into the entire cleanworking area, it is important that, in addition to regular sanitary measures such as cleaning/disinfecting all working areas, technical equipment and storage shelves etc., regular education sessions for laundry employees on proper hand hygiene is undertaken and effective separation of the clean and dirty working areas is achieved.
Ključne besede: textile care, hospital laundry, laundry hygiene, micro-organisms, washing quality parameters, sanitary microbiological parameters, hospital-acquired disease prevention, sanitary measures
Objavljeno: 01.06.2012; Ogledov: 1963; Prenosov: 43
URL Povezava na celotno besedilo

3.
Rotaviral RNA found on various surfaces in a hospital laundry
Sabina Fijan, Andrej Steyer, Mateja Poljšak-Prijatelj, Avrelija Cencič, Sonja Šostar-Turk, Srečko Koren, 2008, izvirni znanstveni članek

Opis: The aim of this investigative study was to determine the presence of rotaviral RNA at various control points (CP) of a hospital laundry. One of the possible sources of hospital infections is inappropriately laundered and disinfected hospital textiles. RT-PCR and nested PCR for gene amplification using specific primers following RNA isolation were used to determine the presence of rotaviral RNA on swabs. In addition, rotavirus suspensions were inoculated on marked surfaces as positive controls for different surfaces (cotton textiles, folding table and industrial dryer). Rotaviral RNA was found on various laundry surfaces: technical equipment, storage shelves, transport vehicles, personnel's hands, damp textiles, and folded laundry. Rotaviral RNA was also detected at all positive controls on tested surfaces after 24 h. Based on the results, it is very important to take into consideration the proper handling of textiles after washing as one of the precautions against hospital-acquired infections. This paper reports the presence of rotaviral RNA for the first time on surfaces in laundries and equipment, as well as textiles.
Ključne besede: laundry hygiene, rotaviruses, hospital-acquired disease prevention, sanitary measures, occupational health
Objavljeno: 01.06.2012; Ogledov: 1746; Prenosov: 48
URL Povezava na celotno besedilo

4.
Antimicrobial disinfection effect of a laundering procedure for hospital textiles against various indicator bacteria and fungi using different substrates for simulating human excrements
Sabina Fijan, Srečko Koren, Avrelija Cencič, Sonja Šostar-Turk, 2007, izvirni znanstveni članek

Opis: Recent studies confirm the increase of nosocomial infections and microbial resistance. One of the possible causes is infected textiles due to inappropriate laundering procedures. Most Slovenian laundries use thermal laundering procedures with high energy and water consumption to disinfect hospital textiles. In addition to this fact, there is an increasing number of hospital textiles composed of cotton/polyester blends that cannot endure high temperatures of thermal disinfection. On the other hand, decreasing the temperature of laundering procedures enhances the possibility of pathogenic microorganisms to survive the laundering procedure. In our research, we determined the antimicrobic laundering effect by simulating a common laundering procedure for hospital textiles in the laboratory washing machine at different temperatures by the use of bioindicators. Enterococcus faecium, Staphylococcus aureus, Mycobacterium terrae, Enterobacter aerogenes, and Pseudomonas aeruginosa were used for determining the antibacterial laundering effect. Candida albicans was used for determining the antifungal laundering effect. Swine blood, artificial sweat, and swine fat were used as substrates for simulating human excrements and were inoculated together with the chosen microorganisms onto cotton pieces to simulate real laundering conditions. It was found that E. faecium, S. aureus, E. aerogenes, and P. aeruginosa survivedat 60 °C, but no microorganisms were found at 75 °C.
Ključne besede: textile care, hospital laundry, laundry hygiene, micro-organisms, bioindicators, disinfection effect, hospital-acquired disease prevention, sanitary measures
Objavljeno: 01.06.2012; Ogledov: 1805; Prenosov: 61
URL Povezava na celotno besedilo

5.
6.
Real-time polymerase chain reaction for quantitative assessment of common pathogens associated with healthcare-acquired infections on hospital textiles
Urška Rozman, Sabina Fijan, Sonja Šostar-Turk, Vid Mlakar, 2013, izvirni znanstveni članek

Opis: A hospital environment may act as a significant reservoir for potential pathogens that can be transmitted with hospital textiles, which could represent a source of healthcare-acquired infections. Quantitative assessment of nosocomial pathogens with real time polymerase chain reaction (qPCR) on textiles can serve to verify the achievement of standards for textile hygiene of hospital laundry that assess the risk for acquiring hospital infection frominappropriately disinfected textiles. The aim of the study was to establish qPCR for quantitative assessment of selected common nosocomial pathogens (Clostridium difficile, Staphylococcus aureus, Klebsiella pneumoniaeand Pseudomonas aeruginosa) on hospital textiles and to compare the efficiency of the molecular method to the standard procedures for evaluating the bio burden of textiles in hospitals. This study demonstrated that presenceof nosocomial pathogens on hospital textiles can be confirmed with qPCR even where conventional techniques do not give any results. qPCR offers apossibility to confirm the presence of microorganisms in dead or viable but non-culturable states that cannot be detected by conventional sampling techniques but may still pose a hazard to public health.
Ključne besede: healthcare-acquired infections, hospital textiles, Clostridium difficile, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa
Objavljeno: 10.07.2015; Ogledov: 639; Prenosov: 62
URL Povezava na celotno besedilo

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Comparison of methods for detection of four common nosocomial pathogens on hospital textiles
Sabina Fijan, Sonja Šostar-Turk, Urška Rozman, 2014, izvirni znanstveni članek

Opis: Introduction: Although the most common vehicle for transmission of health-care acquired infections is the personto- person transmission route, the role of environment should not be ignored and hospital linen may contribute to the spreading of nosocomial infections. The contact plate method and swabbing are common methods for sampling microorganisms on textiles; however, results are available after two days as they are based on incubation followed by phenotypeidentification. An important alternative is using quick wash-off methods followed by PCR detection, which shortens the identification process from two days to a few hours. Methods: The following test microorganisms at different concentrations were inoculated onto textile swatches and dried overnight: Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and Clostridium difficile. RODAC plate sampling as well as a non-destructive wash-off method for capturing microorganisms from the textilesusing a Morapex device were used. The elution suspension from the Morapex device was used for two methods. In the first method, classical incubation on selective media followed by phenotypic identification was used and in the second method DNA was extracted from the elution suspension followed by amplification and agarose gel electrophoresis to visualize amplified products. Conclusions: All chosen bacteria were found using all methods. However, the most sensitive proved to be detection using PCR amplification as we detected the sample with initial concentration of 102 cfu/mL inoculated onto the textile surface before drying. The final detectablerecovered bacterial concentration on textiles was up to 10 cfu/mL.
Ključne besede: health care associated infections, hospital textiles, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Clostridium difficile, Morapex
Objavljeno: 05.04.2017; Ogledov: 604; Prenosov: 170
.pdf Celotno besedilo (811,70 KB)
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9.
Hospital textiles, are they a possible vehicle for healthcare-associated infections?
Sabina Fijan, Sonja Šostar-Turk, 2012, pregledni znanstveni članek

Opis: Textiles are a common material in healthcare facilities; therefore it is important that they do not pose as a vehicle for the transfer of pathogens to patients or hospital workers. During the course of use hospital textiles become contaminated and laundering is necessary. Laundering of healthcare textiles is most commonly adequate, but in some instances, due to inappropriate disinfection or subsequent recontamination, the textiles may become a contaminated inanimate surface with the possibility to transfer pathogens. In this review we searched the published literature in order to answer four review questions: (1) Are there any reports on the survival of microorganisms on hospital textiles after laundering? (2) Are there any reports that indicate the presence of microorganisms on hospital textiles during use? (3) Are there any reports that microorganisms on textiles are a possible source infection of patients? (4) Are there any reports that microorganisms on textiles are a possible source infection for healthcare workers?
Ključne besede: textile hygiene, disinfection, hospital-acquired infections, inanimate surfaces, infection transmission vehicles
Objavljeno: 21.06.2017; Ogledov: 342; Prenosov: 186
.pdf Celotno besedilo (137,40 KB)
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10.
Partial pressure of end-tidal carbon dioxide successful predicts cardiopulmonary resuscitation in the field - a prospective observational study
Miran Kolar, Miljenko Križmarić, Petra Klemen, Štefek Grmec, 2008, izvirni znanstveni članek

Opis: Introduction: The prognosis among patients who suffer out-of-hospital cardiac arrest is poor. Higher survival rates have been observed only in patients with ventricular fibrillation who were fortunate enough to have basic and advanced life support initiated early after cardiac arrest. The ability to predict outcomes of cardiac arrest would be useful for resuscitation. Changes in expired end-tidal carbon dioxide levels during cardiopulmonary resuscitation may be a useful non-invasive predictor of successful resuscitation and survival from cardiac arrest, and help in the termination of cardiopulmonary resuscitation in the field. Methods: This is a prospective observational study of 737 cases of victims who suffered sudden out-of-hospital cardiac arrest. The patients were intubated and the measurements of end-tidal carbon dioxide were performed. Data according to the Utstein criteria, demographic information, medical data and partial pressure of end-tidal carbon dioxide (petCO2) values were collected for each patient in cardiac arrest, by the emergency physician. We presumed that an end-tidal carbon dioxide level of 1.9 kPa (14.3 mmHg) or more after 20 minutes of standard advanced cardiac life support would predict restoration of spontaneous circulation (ROSC). Results: Partial pressure of end-tidal carbon dioxide after 20 minutes of advanced life support averaged 0.92+/- 0.29 kPa (6.9mmHg +/- 2.2 mmHg) in patients who did not have ROSC and 4.36 +/-1.11 kPa (32.8 mmHg +/- 9.1 mmHg) in those who did (p<0,001). End-tidal carbon dioxide values of 1.9 kPa (14.3 mmHg) or less discriminated between the 402 patients with ROSC and 335 patients without ROSC. When a 20-minute end-tidal carbon dioxide value of 1.9 kPa (14.3 mmHg) or less was used as a screening test to predict ROSC, the sensitivity, specificity, positive predictive value, and negative predictive value were all 100 percent. Conclusions: Measurements of end-tidal carbon dioxide levels of more than 1.9 kPa (14.3 mmHg) after 20 minutes should be used to accurately predict ROSC. End-tidal carbon dioxide levels should be monitored during cardiopulmonary resuscitation and considered a useful prognostic value for determining the outcome of resuscitative efforts and termination of cardio-pulmonary resuscitation in the field.
Ključne besede: out-of-hospital cardiac arrest, cardiopulmonary resuscitation, CPR, partial pressure of end-tidal carbon dioxide, PetCO2
Objavljeno: 29.06.2017; Ogledov: 543; Prenosov: 36
.pdf Celotno besedilo (290,02 KB)
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