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1.
Enzymatic reactions in high-pressure membrane reactors
Maja Leitgeb, Mateja Primožič, Željko Knez, 2005, izvirni znanstveni članek

Opis: In the studies on the stability of biocatalysts in a high-pressure batch stirred tank reactor, changes in biocatalyst activity due to pressurizationždepressurization steps were observed. An interesting alternative to overcome this inconvenience is using the high-pressure continuous membrane reactors, where just a single compression and expansion step is necessary. The aim of the research was to carry out the enzyme-catalyzed reactions in high-pressure membrane reactors. At first, high-pressure enzyme-catalyzed hydrolyses of oleyl oleate and sunflower oil were performed in a high-pressure continuous enzymatic flat-shape membrane reactor. Additionally, cellulase from Humicola insolens was covalently immobilized on tubular membrane, and enzyme-catalyzed hydrolysis of carboxy-methyl cellulose was carried out in a high-pressure continuous enzymatic tubular membrane reactor. The membrane-stability tests for flat-shape polysulfon membranes before and after treatment with supercritical CO2 or subcritical propane were done, as well.
Ključne besede: chemical processing, high pressure technology, membrane reactors, biotechnology
Objavljeno: 01.06.2012; Ogledov: 1087; Prenosov: 60
URL Povezava na celotno besedilo

2.
Supercritical fluids as solvents for enzymatic reactions
Maja Leitgeb, Mateja Primožič, Željko Knez, 2007, pregledni znanstveni članek

Opis: Enzymes may act in different solvent systems. Water as the solvent in vivo may be replaced partially or mostly with other solvents, such as micro-emulsions, organic solvents, reversed micelles, ionic liquids and supercritical fluids (SCFs).Several types of enzymatic reactions were performed in SCFs. Influence of SCFs on enzyme stability and activity is presented on different examples; on different reaction systems (hydrolysis, transesterification...) and on the use of non-immobilized (Subtilisin carlsberg, Aspergillus niger...) as well as immobilized enzymes. Several types of high-pressure enzymatic reactors (batch-, stirred-tank-, extractive semibatch-, recirculating batch-, semicontinuous flow-, continuous packed-bed-, and continuous-membrane reactors) have been used for the performance of enzymatic reactions. In the studies on stability of biocatalysts in a high-pressure batch-stirred tank reactor changes in biocatalysts activity due to pressurization/depressurization steps were observed. Interesting alternative to overcome this inconvenience is the use of the high-pressure continuous membrane reactors, where just single compression and expansion step is necessary.
Ključne besede: enzymatic reactions, supercritical carbon dioxide, high-pressure, enzyme stability, high-pressure reactors
Objavljeno: 31.05.2012; Ogledov: 1881; Prenosov: 95
.pdf Celotno besedilo (177,49 KB)
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