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Abstract: In the context of escalating climate challenges, the EnRe conference is dedicated to exploring pathways to climate neutrality and the sustainable green transition. The conference is focused on the development and implementation of innovations supporting the transformation of energy systems, industrial systems, and living systems, all with the goal of creating a sustainable future with net-zero emissions. The conference brings together experts, researchers, policymakers, and business leaders to share their experiences, research, and visions. The aim of the conference is to foster collaboration and exchange of ideas, and to collectively develop comprehensive approaches and strategies for achieving climate neutrality. This conference is not just a knowledge exchange, but also a platform for encouraging concrete actions that will ensure a greenerand more sustainable future for our next generations to come.
Keywords: alternative energy systems, dynamic tariffing, electrical machines and drives, energy conversions, financing energy projects, nuclear energy, conventional energy systems, climate changes - climate pan, mathematical methods in engineering, micro and nano energy, low-carbon technologies and strategies, renewable energy technologies heating and cooling systems, smart buildings, cities and networks, policies and strategies for renewable energy sources, energy efficiency and the climate
Published in DKUM: 17.05.2024; Views: 320; Downloads: 26
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Abstract: Nowadays, pharmaceutical industry is striving to find more biologically relevant solutions and to move from synthetic to biological products. Biopharmaceuticals represent an emerging sector in the pharmaceutical industry and are useful for a wide range of indications, including oncology and rheumatology. The development and optimisation of bioprocesses at laboratory scale is crucial for process understanding and translation into production. Bioprocess development often begins with comparison of several potential clones to be characterized, tested in low-volume cultures with different media or other variables, and then evaluated for growth, productivity, and consistency. The purpose of this master's thesis is to review changes in growth curves, substrate consumption, yield, and product quality of a mammalian cell line when substituting glucose as the main carbon source with alternative energy sources. This master thesis presents an attempt to adapt a mammalian CHO cell line to alternative energy sources. A comparison of the cultivation with maintenance of the CHO mammalian cell line by an intensified and a classical process is also shown. Substrate requirements at different time points of the process were monitored using phenotypic microarrays. Based on the obtained and analyzed results of the phenotypic microarrays, we tried to optimize the cell culture supplementation strategy. We have tried to use small molecules to facilitate adaptation to alternative energy sources. Our results showed that growing CHO cell culture on an alternative energy source-maltose and cell reprogramming with small molecules affects the quality of the product. Trends in cell growth, substrate consumption, by-product excretion and final product concentration are similar between the reference cell cultures and those pre-treated with small molecules. We found that adapting a mammalian CHO cell line to an alternative energy source is a challenging process that would need to be carried out over a long period of time.
Keywords: CHO cells, bioprocess, phenotyping, alternative energy sources, glycan mapping
Published in DKUM: 05.10.2023; Views: 350; Downloads: 85
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