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1.
Bioinspired design of 3D-printed cellular metamaterial prosthetic liners for enhanced comfort and stability
Vasja Plesec, Gregor Harih, 2024, original scientific article

Abstract: Traditional prosthetic liners are often limited in customization due to constraints in manufacturing processes and materials. Typically made from non-compressible elastomers, these liners can cause discomfort through uneven contact pressures and inadequate adaptation to the complex shape of the residual limb. This study explores the development of bioinspired cellular metamaterial prosthetic liners, designed using additive manufacturing techniques to improve comfort by reducing contact pressure and redistributing deformation at the limb–prosthesis interface. The gyroid unit cell was selected due to its favorable isotropic properties, ease of manufacturing, and ability to distribute loads efficiently. Following the initial unit cell identification analysis, the results from the uniaxial compression test on the metamaterial cellular samples were used to develop a multilinear material model, approximating the response of the metamaterial structure. Finite Element Analysis (FEA) using a previously developed generic limb–liner–socket model was employed to simulate and compare the biomechanical behavior of these novel liners against conventional silicone liners, focusing on key parameters such as peak contact pressure and liner deformation during donning, heel strike, and the push-off phase of the gait cycle. The results showed that while silicone liners provide good overall contact pressure reduction, cellular liners offer superior customization and performance optimization. The soft cellular liner significantly reduced peak contact pressure during donning compared to silicone liners but exhibited higher deformation, making it more suitable for sedentary individuals. In contrast, medium and hard cellular liners outperformed silicone liners for active individuals by reducing both contact pressure and deformation during dynamic gait phases, thereby enhancing stability. Specifically, a medium-density liner (10% infill) balanced contact pressure reduction with low deformation, offering a balance of comfort and stability. The hard cellular liner, ideal for high-impact activities, provided superior shape retention and support with lower liner deformation and comparable contact pressures to silicone liners. The results show that customizable stiffness in cellular metamaterial liners enables personalized design to address individual needs, whether focusing on comfort, stability, or both. These findings suggest that 3D-printed metamaterial liners could be a promising alternative to traditional prosthetic materials, warranting further research and clinical validation
Keywords: bioinspired design, metamaterial model, cellular structure, additive manufacturing, lower-limb prosthetic, 3D printing, finite element method
Published in DKUM: 19.09.2024; Views: 0; Downloads: 0
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Thermo-mechanical behavior and strain rate sensitivity of 3D-printed polylactic acid (PLA) below glass transition temperature (Tg)
Vukašin Slavković, Blaž Hanželič, Vasja Plesec, Strahinja Milenković, Gregor Harih, 2024, original scientific article

Abstract: This study investigated the thermomechanical behavior of 4D-printed polylactic acid (PLA), focusing on its response to varying temperatures and strain rates in a wide range below the glass transition temperature (Tg). The material was characterized using tension, compression, and dynamic mechanical thermal analysis (DMTA), confirming PLA’s strong dependency on strain rate and temperature. The glass transition temperature of 4D-printed PLA was determined to be 65 °C using a thermal analysis (DMTA). The elastic modulus changed from 1045.7 MPa in the glassy phase to 1.2 MPa in the rubber phase, showing the great shape memory potential of 4D-printed PLA. The filament tension tests revealed that the material’s yield stress strongly depended on the strain rate at room temperature, with values ranging from 56 MPa to 43 MPA as the strain rate decreased. Using a commercial FDM Ultimaker printer, cylindrical compression samples were 3D-printed and then characterized under thermo-mechanical conditions. Thermo-mechanical compression tests were conducted at strain rates ranging from 0.0001 s−1 to 0.1 s−1 and at temperatures below the glass transition temperature (Tg) at 25, 37, and 50 °C. The conducted experimental tests showed that the material had distinct yield stress, strain softening, and strain hardening at very large deformations. Clear strain rate dependence was observed, particularly at quasi-static rates, with the temperature and strain rate significantly influencing PLA’s mechanical properties, including yield stress. Yield stress values varied from 110 MPa at room temperature with a strain rate of 0.1 s−1 to 42 MPa at 50 °C with a strain rate of 0.0001 s−1. This study also included thermo-mechanical adiabatic tests, which revealed that higher strain rates of 0.01 s−1 and 0.1 s−1 led to self-heating due to non-dissipated generated heat. This internal heating caused additional softening at higher strain rates and lower stress values. Thermal imaging revealed temperature increases of 15 °C and 18 °C for strain rates of 0.01 s−1 and 0.1 s−1, respectively.
Keywords: smart materials, shape memory polymer, 3D printing, 4D printing, thermo-mechanical experiments
Published in DKUM: 29.05.2024; Views: 248; Downloads: 13
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Exploring factors afecting elementary school teachers' adoption of 3D printers in teaching
Branko Anđić, Andrej Šorgo, Christoph Helm, Robert Weinhandl, Vida Lang, 2023, original scientific article

Abstract: Owing to its advantages such as producing durable models and easy accessibility, 3D modeling and printing (3DMP) has become increasingly popular in educational practice and research. However, the results of many studies have shown that the adoption rate of 3DMP among teachers is still low, especially in elementary schools. Therefore, research is needed to expand current knowledge about what influences teachers' decisions to start and continue using 3DMP in elementary schools. To investigate factors that affect elementary school teachers' decisions to use 3D printing in teaching, this study uses a mixed methods research approach combining binary logistic regression with a qualitative thematic analysis approach. Both approaches assembled predictive constructs from a range of theories on (1) technology acceptance and (2) intentions to continue or abandon 3DMP use. Using a sample of 225 elementary teachers from Montenegro, this study empirically concluded that intentions to discontinue 3DMP was slightly more strongly correlated with the predictors (i.e., performance expectancy, effort expectancy, perceived pedagogical impact, personal innovativeness, management support, user interface quality, technology compatibility, social influence, student expectations) than was intentions to continue using 3DMP. Performance expectancy was a significant determinant of teachers’ continued use of a 3DMP approach. The remaining seven factors (constructs) were found to be insignificant predictors. Perceived pedagogical impact and technology compatibility were significantly associated with teachers' decisions to discontinue using 3DMP. Our results also suggest that the time required to use 3DMP during instruction, the impact of 3DMP on the implementation of curriculum activities, and the availability of 3D printers are all relevant factors that influence teachers’' decision to use or abandon 3DMP.
Keywords: elementary school, binary logistic regression, continuance intentions, thematic analysis approach, 3D modelling and printing
Published in DKUM: 26.03.2024; Views: 268; Downloads: 192
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Numerical analysis of a transtibial prosthesis socket using 3D-Printed Bio-Based PLA
Vasja Plesec, Jani Humar, Polona Dobnik-Dubrovski, Gregor Harih, 2023, original scientific article

Abstract: Lower-limb prosthesis design and manufacturing still rely mostly on the workshop process of trial-and-error using expensive unrecyclable composite materials, resulting in time-consuming, material-wasting, and, ultimately, expensive prostheses. Therefore, we investigated the possibility of utilizing Fused Deposition Modeling 3D-printing technology with inexpensive bio-based and bio-degradable Polylactic Acid (PLA) material for prosthesis socket development and manufacturing. The safety and stability of the proposed 3D-printed PLA socket were analyzed using a recently developed generic transtibial numeric model, with boundary conditions of donning and newly developed realistic gait cycle phases of a heel strike and forefoot loading according to ISO 10328. The material properties of the 3D-printed PLA were determined using uniaxial tensile and compression tests on transverse and longitudinal samples. Numerical simulations with all boundary conditions were performed for the 3D-printed PLA and traditional polystyrene check and definitive composite socket. The results showed that the 3D-printed PLA socket withstands the occurring von-Mises stresses of 5.4 MPa and 10.8 MPa under heel strike and push-off gait conditions, respectively. Furthermore, the maximum deformations observed in the 3D-printed PLA socket of 0.74 mm and 2.66 mm were similar to the check socket deformations of 0.67 mm and 2.52 mm during heel strike and push-off, respectively, hence providing the same stability for the amputees. We have shown that an inexpensive, bio-based, and bio-degradable PLA material can be considered for manufacturing the lower-limb prosthesis, resulting in an environmentally friendly and inexpensive solution.
Keywords: 3D printing, bio-based, polylactic acid, PLA, prosthesis, prosthesis socket, numerical model, finite element method
Published in DKUM: 14.03.2024; Views: 208; Downloads: 30
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Functional 3D printed polysaccharide derivative scaffolds for vascular graft application : doctoral disertation
Fazilet Gürer, 2023, doctoral dissertation

Abstract: Tissue engineering (TE) is an interdisciplinary field that aims towards replacement, healing or reconstruction of damaged tissue and organs. Incurable diseases are currently treated with organ transplantation, that have the disadvantages of insufficient donors, immune response, and organ rejection after transplantation. TE imitate the functions of extracellular matrix (ECM) to develop biocompatible/biodegradable scaffolds with appropriate features which are utilized to provide mechanical support, cellular infiltration, migration, and tissue formation, and to mimic the biochemical and biophysical cues of cells. Several fabrication methods have been introduced to mimic the 3D structure of ECM and 3D printing is one of the additive manufacturing techniques, widely used in TE because of its feasibility to build complex tissue constructs and control over fabrication and cell distribution. The polysaccharide-peptide conjugate has gained enormous interest in recent years owing to its biocompatibility, degradability, flexibility, and structural matching to natural proteoglycans. In this context, we reported here on investigation of biocompatibility with HUVECs, surface modification of 3D printed PCL scaffolds with an amine group and chemically crosslinked oxidized HA-amino acid/peptide conjugates (OHACs) was used to develop a novel biomaterial for use as a tissue engineered vascular graft. Modified polysaccharides were characterized with respect to their chemical structure, charge, UV and fluorescence properties and cytotoxicity. The successful conjugation was demonstrated by XPS, and a decrease in the free amine peaks on the surface was observed after conjugation. In addition, the water contact angle measurements showed improved wetting, an indication that the conjugation to the PCL-A surface was successful. Finally, the biocompatibility of the novel scaffolds was characterized by the MTS and the live- dead assay. In both assays, proliferation of cells was observed after 7 days and cell spreading on the surface was detected by phalloidin staining of actin filaments. In conclusion, it was possible to prepare surface-active scaffolds by combining the advantages of biocompatibility and mechanical strength of polysaccharides and polyesters, respectively.
Keywords: 3D tiskanje, karboksimetilceluloza, hialuronska kislina, polikaprolakton, kemija karbodiimida, kemija Shiffove baze, endotelizacija 3D printing, carboxymethyl cellulose, hyaluronic acid, polycaprolactone, carbodiimide chemistry, shiff-base chemistry, endothelialization
Published in DKUM: 06.10.2023; Views: 522; Downloads: 50
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