1. Mesenchymal stem cells isolated from paediatric paravertebral adipose tissue show strong osteogenic potentialJan Rožanc, Lidija Gradišnik, Tomaž Velnar, Minja Gregorič, Marko Milojević, Boštjan Vihar, Boris Gole, Uroš Maver, 2022, izvirni znanstveni članek Opis: Mesenchymal stem cells (MSCs) represent the basis of novel clinical concepts in cellular therapy and tissue regeneration. Therefore, the isolation of MSCs from various tissues has become an important endeavour for stem cell biobanking and the development of regenerative therapies. Paravertebral adipose tissue is readily exposed during spinal procedures in children and could be a viable source of stem cells for therapeutic applications. Here, we describe the first case of MSCs isolated from paravertebral adipose tissue (PV-ADMSCs), obtained during a routine spinal surgery on a child. Using quantitative real-time PCR and flow cytometry, we show that PV-ADMSCs have different levels of stem marker expression compared to the MSCs from other sources while having the highest proliferation rate. Furthermore, we evaluate the multipotency of PV-ADMSCs by the three-lineage (adipogenic, osteogenic and chondrogenic) differentiation and compare it to the multipotency of MSCs from other sources. It was found that the PV-ADMSCs have a strong osteogenic potential in particular. Taken together, our data indicate that PV-ADMSCs meet the criteria for successful cell therapy, defined by the International Society for Cellular Therapy (ISCT), and thus, could provide a source of MSCs that is relatively easy to isolate and expand in culture. Due to their strong osteogenic potential, these cells provide a promising basis, especially for orthopaedic applications.
Ključne besede: mesenchymal stem cells, paediatric surgery, paravertebral adipose tissue, regenerative medicine, stem marker expression, osteogenic potential, differentiation
Objavljeno v DKUM: 16.12.2024; Ogledov: 0; Prenosov: 3
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2. Advanced bio-based polymers for astrocyte cell modelsLidija Gradišnik, Roman Bošnjak, Tina Maver, Tomaž Velnar, 2021, pregledni znanstveni članek Opis: The development of in vitro neural tissue analogs is of great interest for many biomedical engineering applications, including the tissue engineering of neural interfaces, treatment of neurodegenerative diseases, and in vitro evaluation of cell-material interactions. Since astrocytes play a crucial role in the regenerative processes of the central nervous system, the development of biomaterials that interact favorably with astrocytes is of great research interest. The sources of human astrocytes, suitable natural biomaterials, guidance scaffolds, and ligand patterned surfaces are discussed in the article. New findings in this field are essential for the future treatment of spinal cord and brain injuries.
Ključne besede: astrocytes, hydrogels, tissue engineering
Objavljeno v DKUM: 14.08.2024; Ogledov: 118; Prenosov: 7
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3. Biomaterials and host versus graft response : a short reviewTomaž Velnar, Gorazd Bunc, Robert Klobucar, Lidija Gradišnik, 2016, pregledni znanstveni članek Opis: Biomaterials and biotechnology are increasing becoming an important area in modern medicine. The main aim in this area is the development of materials, which are biocompatible to normal tissue. Tissue-implant interactions with molecular, biological and cellular characteristics at the implant-tissue interface are important for the use and development of implants. Implantation may cause an inflammatory and immune response in tissue, foreign body reaction, systemic toxicity and imminent infection. Tissue-implant interactions determine the implant life-period. The aims of the study are to consider the biological response to implants. Biomaterials and host reactions to implants and their mechanisms are also briefly discussed.
Ključne besede: host versus graft disease, GVHD, biomaterial, wound healing, transplant, tissue, prosthetic, implants, biological response, complications
Objavljeno v DKUM: 03.08.2017; Ogledov: 1466; Prenosov: 211
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5. Is tissue augmentation a reality in biosurgery? An experimental study of endothelial cell invasion into tissue fillerTomaž Velnar, Vladimir Smrkolj, Marjan Rupnik, Lidija Gradišnik, 2013, izvirni znanstveni članek Opis: New therapeutic approaches for wound treatment are evolving. Non healing wounds in oncology and after trauma may be cured by a novel technique of tissue augmentation with soft tissue fillers. The principle resides in filling the wound with collagen filler in order to seal the defect and promote healing. Successful angiogenesis forms the basis of tissue filler survival and determines the outcome of the healing process. During this study, basic data about endothelial cell invasion into collagen-made substratum was collected that could be used for neoangiogenesis studies in tissue augmentation techniques for large wound defect treatment. In the in vitro assay, the human umbilical vein endothelial cells (HUVEC) grow into a three-dimensional framework of collagenous tissue fillers, forming the basic step for angiogenesis. After heparins were used as chemotactic agents, a typical bell-shaped relationship between chemotaxis and agent concentrations was found. Significant cell infiltration was present in the assays with chemotactic agents. These observations support the potential for tissue augmentation with soft tissue fillers that could be used in acute and chronic non healing traumatic and oncology wounds after extensive surgical resections and radiotherapy.
Ključne besede: angiogenesis, cell invasion, tissue augmentation, tissue filler, wound healing
Objavljeno v DKUM: 10.07.2015; Ogledov: 1535; Prenosov: 104
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