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Opis: Sustainable extraction methods for natural extracts are crucial for mitigating environmental impact. Strategies that focus on waste reduction and resource efficiency contribute to long-term conservation. Hemp seeds, which were previously treated as waste product, are now valued for their nutrition and functional properties. This study investigated sustainable extraction methods for obtaining natural extracts from hemp seeds, with a focus on reducing environmental impact through efficient resource utilization and waste reduction. The extraction methods compared included supercritical fluid extraction (SFE), Soxhlet extraction (SOX), ultrasound-assisted extraction (UAE), cold maceration (CM), and cold pressing (CP). The contents of antioxidants, total phenols, and fatty acids in the extracts were analyzed via gas chromatography–mass spectrometry (GC–MS). The extracts were subsequently formulated into organogels to evaluate the stability of the fatty acids. The results showed that SFE and CP were the most efficient and environmentally friendly methods, with SFE allowing complete separation of the solvent from the extract. CP was also found to be effective and environmentally friendly. The study confirmed that formulating extracts in organogels effectively inhibited fatty acid oxidation, although a reduction in fatty acid content was observed during gel production. Overall, the formulation of extracts in organogels increased their stability, especially in preventing fatty acid degradation.
Ključne besede: supercritical fluid extraction, ultrasound extraction, cold pressing method, fatty acids, stability of formulations
Objavljeno v DKUM: 15.10.2025; Ogledov: 0; Prenosov: 3
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Opis: Micellar or mycelial membranes from medicinal mushrooms are self-growing fibrous polymeric biocomposites that are biocompatible, biodegradable, cost-effective, and environmentally friendly. In this study, the cultivation process for the medicinal mushrooms Ganoderma lucidum and Pleurotus ostreatus has been optimized via submerged cultivation to maximize growth and promote the formation of micellar membranes with high water-absorption capacity. Optimal growth conditions were achieved at an alkaline pH in a medium containing malt extract for G. lucidum, while for P. ostreatus, these were in a glucose-enriched medium. The hydrophilic underside of the micellar membranes led to a high-water uptake capacity. These membranes exhibited a broad spectrum of functional groups, thermal stability with decomposition temperatures above 260 °C, and a fibrous and porous structure. The micellar membranes from both mushrooms were additionally functionalized with mango peel extract (MPE), resulting in a uniform and gradual release profile, which is an important novelty. They also showed successful antimicrobial activity against Escherichia coli and Staphylococcus aureus growth. MPE-functionalized micellar membranes are, therefore, innovative biocomposites suitable for various biomedical applications. As they mimic the extracellular matrix of the skin, they are a promising material for tissue engineering, wound healing, and advanced skin materials applications.
Ključne besede: medicinal mushrooms, Ganoderma lucidum, Pleurotus ostreatus
Objavljeno v DKUM: 04.09.2025; Ogledov: 0; Prenosov: 3
Celotno besedilo (6,62 MB)

Opis: The aim of this review was to investigate the potential use of Castanea sativa wood extract as a food supplement and to evaluate its beneficial properties for human health. The results of the limited amount of studies suggest promising properties, including potential anti-inflammatory effects. The literature indicates that the extract, which is rich in bioactive compounds such as tannins, offers promising therapeutic possibilities for the treatment of conditions associated with chronic inflammation. Consequently, interest in its use in food and pharmaceuticals is growing. Phytochemical studies have reported antioxidant and antimicrobial activities, and anti-inflammatory, anticancer, hypolipidemic, hypoglycemic, and neuroprotective activities. A suitable extraction method and solvent is crucial for the isolation of bioactive compounds, being green extraction technologies outstanding for the industrial recovery of chestnut wood’s bioactive compounds. Nevertheless, it is important to emphasize the importance of adhering to regulatory guidelines and obtaining the necessary approvals from regulatory authorities to ensure product safety and compliance. The regulation of herbal medicinal products with proven efficacy and traditional herbal medicinal products is well defined, monitored by authorized bodies, and subject to strict control measures. It is noteworthy that medicinal products are subject to stringent quality testing to ensure safety and efficacy in use, whereas there are no comparable regulatory standards and specific labeling requirements for dietary supplements. When using herbal products, compliance with established standards in health research is essential.
Ključne besede: Castanea sativa, wood extracts, tannins, antioxidat activity, anti-inflammatory activity, antimicrobial activity
Objavljeno v DKUM: 30.07.2025; Ogledov: 0; Prenosov: 7
Celotno besedilo (1,38 MB)
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Opis: Proper processing of natural material is crucial to obtain an extract with high content of biologically active components. Dried, grinded ginger roots were extracted by ultrasonic method and supercritical extraction with CO2 . The aim of the study was to determine if a mixture of the two types of extracts attained by different methods and solvents exhibits better bioavailability than each extract itself. Therefore, both extracts were analytically evaluated and then mixed in a ratio of 1:1. The supercritical extract (SCG extract) and the mixed extract (mixG extract) had high antioxidant activity (78% and 73%) and total phenols (827 mg/g ext. and 1455 mg/g ext.), which is also consistent with the levels of gingerol (303 mg/g ext. and 271 g/g ext.) and shogaol (111 mg/g ext. and 100 g/g ext.) in the extracts. In comparison to both pure extracts higher levels of total phenols were found in the extract mixG. This could be the reason for the significant inhibition of melanoma cells and antimicrobial potential (against Staphylococcus aureus, Escherichia coli, and Candida albicans). The combination of the extracts resulted in a significant increase in the inhibition of selected microbial and melanoma cells WM-266-4 compared to the control. Cell viability decreased below 60% when mixG extract was applied. Antimicrobial activity has been confirmed.
Ključne besede: ginger, supercritical fluid extraction, ultrasound-assisted extraction, antimicrobial activity
Objavljeno v DKUM: 09.05.2025; Ogledov: 0; Prenosov: 12
Celotno besedilo (1,59 MB)
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Opis: In this research, a new approach to enzyme inactivation in flour was presented by supercritical technology, considered a sustainable technology with lower energy consumption compared to other technologies that use ultra-high temperature processing. Total protein concentration and the activity of enzymes α-amylase, lipase, peroxidase, polyphenol oxidase, and protease were determined in flour pre-treated with scCO2. During the study, it was observed that the activity of enzymes such as lipase and polyphenol oxidase, was significantly reduced under certain conditions of scCO2 treatment, while the enzymes α-amylase and protease show better stability. In particular, polyphenol oxidase was effectively inactivated below the 60% of preserved activity at 200 bar and 3 h, whereas α-amylase under the same conditions retained its activity. Additionally, the moisture content of the scCO2-treated spelt flour was reduced by 5%, and the fat content was reduced by 58%, while the quality of scCO2-treated flour was maintained. In this regard, the sustainable scCO2 process could be a valuable tool for controlling the enzymatic activity of spelt flour since the use of scCO2 technology has a positive effect on the quality of flour, which was verified by the baking performance of spelt flour with the baked spelt bread as an indicator of quality.
Ključne besede: food treatment, spelt flour, proteins, supercritical carbon dioxide, enzyme inactivation, defatted flour, carbon dioxide solubility
Objavljeno v DKUM: 17.04.2025; Ogledov: 0; Prenosov: 4
Celotno besedilo (2,72 MB)
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