1. Contrast-enhanced ultrasound imaging of the optic nerve sheath diameter : a proof of concept studyAndrej Bergauer, Gregor Prosen, Nina Kobilica, Vojko Flis, Marko Jevšek, Tomaž Šeruga, 2017, izvirni znanstveni članek Ključne besede: oko, magnetna resonanca, ovojnica optičnega živca, ultrazvok s kontrastom, anatomija ožilja
Objavljeno v DKUM: 09.04.2024; Ogledov: 185; Prenosov: 10
 Celotno besedilo (274,53 KB)
Gradivo ima več datotek! Več...
Gradivo je zbirka in zajema 1 gradivo! |
2. Intracellular serotonin modulates insulin secretion from pancreatic ß-cells by protein serotonylationNils Paulmann, Maik Grohmann, Jörg-Peter Voigt, Bettina Bert, Jakob Vowinckel, Michael Bader, Maša Skelin, Marko Jevšek, Heidrun Fink, Marjan Rupnik, Diego Walther, 2009, izvirni znanstveni članek Opis: While serotonin (5-HT) co-localization with insulin in granules of pancreatic ß-cells was demonstrated more than three decades ago, its physiological role in the etiology of diabetes is stili unclear. We combined biochemical and electrophysiological analyses of mice selectively deficient in peripheral tryptophan hydroxylase (Tph1-/-) and 5-HT to show that intracellular 5-HT regulates insulin secretion. We found that these mice are diabetic and have an impaired insulin secretion due to the lack of 5-HT in the pancreas. The pharmacological restoration of peripheral 5-HT levels rescued the impaired insulin secretion in vivo. These findings were further evidenced by patch clamp experiments with isolated Tph1-/- ß-cells, which clearly showed that the secretory defect is downstream of Ca2+ -signaling and can be rescued by direct intracellular application of 5-HT via the clamp pipette. In elucidating the underlying mechanism further, we demonstrate the covalent coupling of 5-HT by transglutaminases during insulin exocytosis to two key players in insulin secretion, the small GTPases Rab3a and Rab27a. This renders them constitutively active in a receptor-independent signaling mechanism we have recently termed serotonylation. Concordantly, an inhibition of such activating serotonylation in ß-cells abates insulin secretion. We also observed inactivation of serotonylated Rab3a by enhanced proteasomal degradation, which is in line with the inactivation of other serotonylated GTPases. Our results demonstrate that 5-HT regulates insulin secretion by serotonylation of GTPases within pancreatic ß-cells and suggest that intracellular 5-HT functions in various microenvironments via this mechanism in concert with the known receptor-mediated signaling.
Ključne besede: insulin secretion, serotonin, insulin, glucose, diabetes mellitus, guanosine triphosphatase, exocytosis, pancreas
Objavljeno v DKUM: 16.06.2017; Ogledov: 2195; Prenosov: 204
Celotno besedilo (774,75 KB)
Gradivo ima več datotek! Več... |
3. Treatment of tongue cavernous haemangioma with direct puncture and sclerotization with ethanolTomaž Šeruga, Jernej Lučev, Marko Jevšek, 2015, strokovni članek Opis: Background. Haemangiomas of tongue are rare type of malformations. They can be treated mostly conservatively but in some cases they need more aggressive treatment with preoperative intra arterial embolization and surgical resection. Lesions of tongue that are localized superficially can also be treated with direct puncture and injection of sclerosing agent (absolute ethanol). Case report. We present a case of a 48 years old female patient, where we performed embolization of cavernous haemangioma with mixture of absolute ethanol and oil contrast. After the procedure the patient received analgetics and antioedematous therapy. After the sclerotization the planed surgery was abandoned. Control MRI examinations 6 and 12 months after the procedure showed only a small remnant of haemangioma and no signs of a larger relapse. Conclusion. In our case the direct puncture of haemangioma and sclerotherapy with ethanol proved to be a safe and effective method to achieve preoperative devascularization of the lesion. Direct puncture of the lesion is not limited by the anatomy of the vessels or vasospasm, which can occur during the intra-arterial approach.
Ključne besede: etanol, hemangiom, jezik, kontrastno sredstvo, punkcija, sklerotizacija, vaskularne nepravilnosti
Objavljeno v DKUM: 30.12.2015; Ogledov: 1932; Prenosov: 213
 Povezava na celotno besedilo |
4. Vpeljava in optimizacija in-vitro metode za določanje hemokompatibilnosti modificiranih polietilenteraftalnih površinJan Stana, Domen Stropnik, Simona Strnad, Tea Indest, Marko Jevšek, Gorazd Košir, 2009, izvirni znanstveni članek Ključne besede: biomaterials, poly(ethyleneterephthalate), hemocompatibility, surface modification, in-vitro method
Objavljeno v DKUM: 10.07.2015; Ogledov: 2121; Prenosov: 56
Povezava na celotno besedilo |
5. |
