Independent clinical research may alleviate disparities in cancer treatmentMatjaž Zwitter
, 2016, izvirni znanstveni članek
Opis: Disparities in cancer care are a reality of the modern world. Unfortunately, current clinical research is in the hands of for-profit pharmaceutical companies and of researchers from the developed world. Problems specific to cancer care in developing countries and among deprivileged populations are ignored. Independent clinical research can offer new valuable knowledge and identify affordable and cost-effective treatments. As such, research not depending on commercial sponsors should become one of the important avenues to alleviate the problem of cancer disparities.
Ključne besede: clinical research, disparities in cancer care, cost-effective treatment
Objavljeno: 04.08.2017; Ogledov: 304; Prenosov: 17
Celotno besedilo (472,46 KB)
Exploring ComQXPA quorum-sensing diversity and biocontrol potential of Bacillus spp. isolates from tomato rhizoplaneAnna Oslizlo
, Polonca Štefanič
, Sabina Vatovec
, Sara Beigot Glaser
, Maja Rupnik
, Ines Mandić-Mulec
, 2015, izvirni znanstveni članek
Opis: Bacillus subtilis is a widespread and diverse bacterium t exhibits a remarkable intraspecific diversity of the ComQXPA quorum-sensing (QS) system. This manifests in the existence of distinct communication groups (pherotypes) that can efficiently communicate within a group, but not between groups. Similar QS diversity was also found in other bacterial species, and its ecological and evolutionary meaning is still being explored. Here we further address the ComQXPA QS diversity among isolates from the tomato rhizoplane, a natural habitat of B. subtilis, where these bacteria likely exist in their vegetative form. Because this QS system regulates production of anti-pathogenic and biofilm-inducing substances such as surfactins, knowledge on cell–cell communication of this bacterium within rhizoplane is also important from the biocontrol perspective. We confirm the presence of pherotype diversity within B. subtilis strains isolated from a rhizoplane of a single plant. We also show that B. subtilis rhizoplane isolates show a remarkable diversity of surfactin production and potential plant growth promoting traits. Finally, we discover that effects of surfactin deletion on biofilm formation can be strain specific and unexpected in the light of current knowledge on its role it this process.
Ključne besede: Bacillus subtilis, quorum sensing, cellular communication, rhizosphere
Objavljeno: 04.08.2017; Ogledov: 233; Prenosov: 28
Celotno besedilo (944,16 KB)
Comparative genome-wide analysis of small RNAs of major Gram-positive pathogensMobarak Mraheil
, André Billion
, Carsten Kuenne
, Jordan Pischimarov
, Bernd Kreikemayer
, Sussane Engelmann
, Axel Hartke
, Jean-Christophe Giard
, Maja Rupnik
, Sonja Vorwerk
, Markus Beier
, Julia Retey
, Thomas Hartsch
, Anette Jacob
, Franz Cemič
, Jürgen Hemberger
, Trinad Chakraborty
, Torsten Hain
, 2010, pregledni znanstveni članek
Opis: In the recent years, the number of drug- and multi-drug-resistant microbial strains has increased rapidly. Therefore, the need to identify innovative approaches for development of novel anti-infectives and new therapeutic targets is of high priority in global health care. The detection of small RNAs (sRNAs) in bacteria has attracted considerable attention as an emerging class of new gene expression regulators. Several experimental technologies to predict sRNA have been established for the Gram-negative model organism Escherichia coli. In many respects, sRNA screens in this model system have set a blueprint for the global and functional identification of sRNAs for Gram-positive microbes, but the functional role of sRNAs in colonization and pathogenicity for Listeria monocytogenes, Staphylococcus aureus, Streptococcuspyogenes, Enterococcus faecalis and Clostridium difficile is almost completely unknown. Here, we report the current knowledge about the sRNAs of these socioeconomically relevant Gram-positive pathogens, overview the state-of-the-art high-throughput sRNA screening methods and summarize bioinformatics approaches for genome-wide sRNA identification and target prediction. Finally, we discuss the use of modified peptide nucleic acids (PNAs) as a novel tool to inactivate potential sRNA and their applications in rapid and specific detection of pathogenic bacteria.
Ključne besede: anti-infectiveness, peptide nucleic acids, sRNA
Objavljeno: 04.08.2017; Ogledov: 235; Prenosov: 36
Celotno besedilo (698,61 KB)
Factors associated with health-related quality of life among university studentsZalika Klemenc-Ketiš
, Janko Kersnik
, Ksenija Eder
, Dušan Colarič
, 2011, izvirni znanstveni članek
Opis: Introduction: University students are subjected to different kinds of stressors, i.e.academic pressures, social issues and financial problems. This can affect their academic achievements and quality of life.
Objective: The aim of this study was to determine the health-related quality of life of university students, and how it is affected by the presence of chronic diseases, mental disorders comorbidity, and patterns of medical services' use.
Methods: This web-based study included a sample of 1,410 Slovenian university students. We used a self-administered questionnaire, containing a sheet with demographic data, Zung's self-assessment inventories about anxiety and depression, and EQ-5D questionnaire. The main outcome measures were scores on EQ-5D part and VAS part of the EQ-5D questionnaire.
Results: Independent factors associated with the health-related quality of life of university students, were the presence of chronic pain, the presence of depression and anxiety, need for urgent medical help and at least one visit to a clinical specialist in the past year. The independent factors associated with the health status of university students were the presence of chronic diseases, chronic pain, depression and anxiety, a visit to a clinical specialist, a need for urgent medical help and a visit to an emergency unit in the past year.
Conclusion: Health-related quality of life of university students can be seriously affected by the presence of mental disorders and chronic pain. Appropriate health-related measures should be adopted to achieve early recognition of worse health-related quality of life, the presence of mental disorders and other chronic conditions, and to enable their effective treatment.
Ključne besede: quality of life, students, chronic disease, pain, depression, anxiety
Objavljeno: 04.08.2017; Ogledov: 153; Prenosov: 42
Celotno besedilo (192,97 KB)
Deficiency areas in decision making in undergraduate medical studentsZalika Klemenc-Ketiš
, Janko Kersnik
, 2014, izvirni znanstveni članek
Opis: Background: In family medicine, decisions can be difficult due to the early presentation of often poorly developed symptoms or the presentation of undifferentiated conditions that require competencies unique to family medicine, such as; primary care management, specific problem-solving skills, and a comprehensive and holistic approach to be taught to medical students.
Purpose: The aim of this study was to assess the decision-making process covering all theoretical aspects of family practice consultation and to recognize possible areas of deficiency in undergraduate medical students.
Materials and methods: This was a cross-sectional, observational study performed at the Medical School of the University of Maribor in Slovenia. The study population consisted of 159 fourth-year medical students attending a family medicine class. The main outcome measure was the scores of the students' written reports on solving the virtual clinical case. An assessment tool consisted of ten items that could be graded on a 5-point Likert scale.
Results: The final sample consisted of 147 (92.5%) student reports. There were 95 (64.6%) female students in the sample. The mean total score on the assessment scale was 35.1+-7.0 points of a maximum 50 points. Students scored higher in the initial assessment items and lower in the patient education/involvement items. Female students scored significantly higher in terms of total assessment score and in terms of initial assessment and patient education/involvement.
Conclusion: Undergraduate medical education should devote more time to teaching a comprehensive approach to consultation, especially modification of the health behavior of patients and opportunistic health promotion to patients. Possible sex differences in students' performance should be further evaluated.
Ključne besede: assessment, family medicine, undergraduate medical education, decision making
Objavljeno: 03.08.2017; Ogledov: 154; Prenosov: 37
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Optimization of induction of mild therapeutic hypothermia with cold saline infusionJure Fluher
, Andrej Markota
, Andraž Stožer
, Andreja Sinkovič
, 2015, izvirni znanstveni članek
Opis: Cold fluid infusions can be used to induce mild therapeutic hypothermia after cardiac arrest. Fluid temperature higher than 4°C can increase the volume of fluid needed, prolong the induction phase of hypothermia and thus contribute to complications. We performed a laboratory experiment with two objectives. The first objective was to analyze the effect of wrapping fluid bags in ice packs on the increase of fluid temperature with time in bags exposed to ambient conditions. The second objective was to quantify the effect of insulating venous tubing and adjusting flow rate on fluid temperature increase from bag to the level of an intravenous cannula during a simulated infusion. The temperature of fluid in bags wrapped in ice packs was significantly lower compared to controls at all time points during the 120 minutes observation. The temperature increase from the bag to the level of intravenous cannula was significantly lower for insulated tubing at all infusion rates (median temperature differences between bag and intravenous cannula were: 8.9, 4.8, 4.0, and 3.1°C, for non-insulated and 5.9, 3.05, 1.1, and 0.3°C, for insulated tubing, at infusion rates 10, 30, 60, and 100 mL/minute, respectively). The results from this study could potentially be used to decrease the volume of fluid infused when inducing mild hypothermia with an infusion of cold fluids.
Ključne besede: cardiac arrest, intravenous infusion, therapeutic hypothermia
Objavljeno: 03.08.2017; Ogledov: 179; Prenosov: 35
Celotno besedilo (232,18 KB)
Biomaterials and host versus graft responseTomaž Velnar
, Gorazd Bunc
, Robert Klobucar
, Lidija Gradišnik
, 2016, pregledni znanstveni članek
Opis: Biomaterials and biotechnology are increasing becoming an important area in modern medicine. The main aim in this area is the development of materials, which are biocompatible to normal tissue. Tissue-implant interactions with molecular, biological and cellular characteristics at the implant-tissue interface are important for the use and development of implants. Implantation may cause an inflammatory and immune response in tissue, foreign body reaction, systemic toxicity and imminent infection. Tissue-implant interactions determine the implant life-period. The aims of the study are to consider the biological response to implants. Biomaterials and host reactions to implants and their mechanisms are also briefly discussed.
Ključne besede: host versus graft disease, GVHD, biomaterial, wound healing, transplant, tissue, prosthetic, implants, biological response, complications
Objavljeno: 03.08.2017; Ogledov: 217; Prenosov: 22
Celotno besedilo (815,30 KB)
Vgradnja magnetnih nanodelcev v modelne medicinske obloge za kombinirano zdravljenje kožnih oblik rakaKristjan Orthaber
, Matevž Pristovnik
, Kristijan Skok
, 2016, druge monografije in druga zaključena dela
Ključne besede: kožni rak, medicinske obloge, tanki filmi, biokompatibilni polimeri, nadzorovano sproščanje, in vitro celični testi
Objavljeno: 03.08.2017; Ogledov: 185; Prenosov: 65
Celotno besedilo (2,24 MB)