Optimization of induction of mild therapeutic hypothermia with cold saline infusionJure Fluher
, Andrej Markota
, Andraž Stožer
, Andreja Sinkovič
, 2015, izvirni znanstveni članek
Opis: Cold fluid infusions can be used to induce mild therapeutic hypothermia after cardiac arrest. Fluid temperature higher than 4°C can increase the volume of fluid needed, prolong the induction phase of hypothermia and thus contribute to complications. We performed a laboratory experiment with two objectives. The first objective was to analyze the effect of wrapping fluid bags in ice packs on the increase of fluid temperature with time in bags exposed to ambient conditions. The second objective was to quantify the effect of insulating venous tubing and adjusting flow rate on fluid temperature increase from bag to the level of an intravenous cannula during a simulated infusion. The temperature of fluid in bags wrapped in ice packs was significantly lower compared to controls at all time points during the 120 minutes observation. The temperature increase from the bag to the level of intravenous cannula was significantly lower for insulated tubing at all infusion rates (median temperature differences between bag and intravenous cannula were: 8.9, 4.8, 4.0, and 3.1°C, for non-insulated and 5.9, 3.05, 1.1, and 0.3°C, for insulated tubing, at infusion rates 10, 30, 60, and 100 mL/minute, respectively). The results from this study could potentially be used to decrease the volume of fluid infused when inducing mild hypothermia with an infusion of cold fluids.
Ključne besede: cardiac arrest, intravenous infusion, therapeutic hypothermia
Objavljeno: 03.08.2017; Ogledov: 70; Prenosov: 3
Celotno besedilo (232,18 KB)
Biomaterials and host versus graft responseTomaž Velnar
, Gorazd Bunc
, Robert Klobucar
, Lidija Gradišnik
, 2016, pregledni znanstveni članek
Opis: Biomaterials and biotechnology are increasing becoming an important area in modern medicine. The main aim in this area is the development of materials, which are biocompatible to normal tissue. Tissue-implant interactions with molecular, biological and cellular characteristics at the implant-tissue interface are important for the use and development of implants. Implantation may cause an inflammatory and immune response in tissue, foreign body reaction, systemic toxicity and imminent infection. Tissue-implant interactions determine the implant life-period. The aims of the study are to consider the biological response to implants. Biomaterials and host reactions to implants and their mechanisms are also briefly discussed.
Ključne besede: host versus graft disease, GVHD, biomaterial, wound healing, transplant, tissue, prosthetic, implants, biological response, complications
Objavljeno: 03.08.2017; Ogledov: 80; Prenosov: 3
Celotno besedilo (815,30 KB)
Vgradnja magnetnih nanodelcev v modelne medicinske obloge za kombinirano zdravljenje kožnih oblik rakaKristjan Orthaber
, Matevž Pristovnik
, Kristijan Skok
, 2016, druge monografije in druga zaključena dela
Ključne besede: kožni rak, medicinske obloge, tanki filmi, biokompatibilni polimeri, nadzorovano sproščanje, in vitro celični testi
Objavljeno: 03.08.2017; Ogledov: 66; Prenosov: 5
Celotno besedilo (2,24 MB)
Isolation and characterization of human articular chondrocytes from surgical waste after total knee arthroplasty (TKA)Jakob Naranda
, Lidija Gradišnik
, Mario Gorenjak
, Matjaž Vogrin
, Uroš Maver
, 2017, izvirni znanstveni članek
Opis: BACKGROUND: Cartilage tissue engineering is a fast-evolving field of biomedical engineering, in which the chondrocytes represent the most commonly used cell type. Since research in tissue engineering always consumes a lot of cells, simple and cheap isolation methods could form a powerful basis to boost such studies and enable their faster progress to the clinics. Isolated chondrocytes can be used for autologous chondrocyte implantation in cartilage repair, and are the base for valuable models to investigate cartilage phenotype preservation, as well as enable studies of molecular features, nature and scales of cellular responses to alterations in the cartilage tissue.
METHODS: Isolation and consequent cultivation of primary human adult articular chondrocytes from the surgical waste obtained during total knee arthroplasty (TKA) was performed. To evaluate the chondrogenic potential of the isolated cells, gene expression of collagen type 2 (COL2), collagen 1 (COL1) and aggrecan (ACAN) was evaluated. Immunocytochemical staining of all mentioned proteins was performed to evaluate chondrocyte specific production.
RESULTS: Cartilage specific gene expression of COL2 and ACAN has been shown that the proposed protocol leads to isolation of cells with a high chondrogenic potential, possibly even specific phenotype preservation up to the second passage. COL1 expression has confirmed the tendency of the isolated cells dedifferentiation into a fibroblast-like phenotype already in the second passage, which confirms previous findings that higher passages should be used with care in cartilage tissue engineering. To evaluate the effectiveness of our approach, immunocytochemical staining of the evaluated chondrocyte specific products was performed as well.
DISCUSSION: In this study, we developed a protocol for isolation and consequent cultivation of primary human adult articular chondrocytes with the desired phenotype from the surgical waste obtained during TKA. TKA is a common and very frequently performed orthopaedic surgery during which both femoral condyles are removed. The latter present the ideal source for a simple and relatively cheap isolation of chondrocytes as was confirmed in our study.
Ključne besede: aggrecan, collagen 2, gene expression, human articular chondrocytes, isolation protocol, phenotype preservation, TKA, total knee arthroplasty
Objavljeno: 02.08.2017; Ogledov: 101; Prenosov: 2
Celotno besedilo (42,50 MB)