Iskanje po katalogu digitalne knjižnice

Opis: Lichen sclerosus (LS) is a chronic, relapsing skin disease that predominantly affects the perineal and genital regions, although extragenital manifestations can occur. Despite its significant impact on patients’ quality of life, particularly affecting sexual and urinary function, LS remains underdiagnosed. Multiple factors, including genetic predisposition, hormonal changes, immunological abnormalities, trauma, and urine irritation, contribute to its development and persistence. This review aims to clarify the complex pathophysiology of LS by exploring three main mechanisms: autoimmune dysregulation, sclerotic tissue formation, and oxidative stress. Autoimmune dysregulation involves T-cell infiltration and the roles of miR-155 and extracellular matrix protein 1 dysfunction, leading to chronic inflammation. miR-155 contributes to sclerotic tissue formation alongside galectin-7, promoting fibroblast proliferation and collagen synthesis. Oxidative stress results in tissue damage, autoimmunity, chronic inflammation, and an increased risk of carcinogenesis. Understanding these mechanisms is crucial for developing targeted therapies and improving LS management. Further research is needed to unravel the genetic basis, immune responses, and interactions between key mediators, ultimately advancing innovative therapeutic strategies and precision medicine in LS.
Ključne besede: lichen sclerosus, autoimmune dysregulation, sclerotic tissue, oxidative stress, genital disease
Objavljeno v DKUM: 15.09.2025; Ogledov: 0; Prenosov: 45
Celotno besedilo (1,96 MB)
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Opis: Cellulose and its derivatives are increasingly explored in biomedical applications due to their biocompatibility,biodegradability, and mechanical performance. In regenerative medicine, aerogel scaffolds with tunable morphology and compositionare highly valued for their ability to support tissue regeneration. Three-dimensional (3D) printing offers an effective method tofabricate aerogels with hierarchical pore structures, comprising interconnected macropores and mesopores, that are crucial for tissueengineering. For clinical use, 3D printing should ensure the structural integrity of printed structures and achieve a printing resolutionthat allows for customization. In this work, the X-aerogel technology, implemented via polyurea cross-linking, was applied to 3D-printed cellulose structures, thereby expanding the potential applications of both technologies. Specifically, 3D-printedmethylcellulose (MC) and MC doped with bacterial cellulose nanofiber (MCBCf) gels were cross-linked with an aliphaticpolyurea, yielding, after supercritical drying, the corresponding (X-MC and X-MCBCf) aerogels. Elaborate characterization withATR-FTIR, XPS, ToF-SIMS, N2 porosimetry, He pycnometry, and SEM confirmed the formation of polyurea on the biopolymerframework, reinforcing the structure and improving the mechanical properties without altering the morphology or texturalcharacteristics of the materials. A significant outcome of cross-linking with polyurea is the long-term stability of X-MC and X-MCBCf aerogels in water, in contrast to their native counterparts, and their capacity to absorb water up to 1800% w/w within only 2h. Preliminary biological evaluation of the materials, including in vitro (cell compatibility, hemolytic activity), in ovo (HET-CAM),and in vivo (A. salina model) tests, showed good cell viability, blood compatibility, and safety for living organisms. From afundamental materials perspective, the most important finding of this work is the disproportionally high stability of X-MC and X-MCBCf in physiological environments, achieved with only a minimal (almost undetectable) amount of cross-linking polyurea. Froman application standpoint, the findings of this study, collectively, position these aerogels as sustainable and promising candidates fortissue engineering scaffolds.
Ključne besede: 3D printing, aerogels, cellulose, methylcellulose, polyurea, tissue engineering, X-aerogels
Objavljeno v DKUM: 30.05.2025; Ogledov: 0; Prenosov: 6
Celotno besedilo (7,71 MB)

Opis: Modern pig breeds, which have been genetically improved to achieve fast growth and a lean meat deposition, differ from local pig breeds with respect to fat deposition, fat specific metabolic characteristics and various other properties. The present review aimed to elucidate the mechanisms underlying the differences between fatty local and modern lean pig breeds in adipose tissue deposition and lipid metabolism, taking into consideration morphological, cellular, biochemical, transcriptomic and proteomic perspectives. Compared to modern breeds, local pig breeds accumulate larger amounts of fat, which generally contains more monounsaturated and saturated fatty acids; they exhibit a higher adipocyte size and higher activity of lipogenic enzymes. Studies using transcriptomic and proteomic approaches highlighted several processes like immune response, fatty-acid turn-over, oxidoreductase activity, mitochondrial function, etc. which differ between local and modern pig breeds.
Ključne besede: pig, adipose tissue, local breeds, modern breeds, cellularity, transcriptome, proteom, adaptation
Objavljeno v DKUM: 06.02.2025; Ogledov: 0; Prenosov: 7
Celotno besedilo (1,72 MB)
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Opis: Mesenchymal stem cells (MSCs) represent the basis of novel clinical concepts in cellular therapy and tissue regeneration. Therefore, the isolation of MSCs from various tissues has become an important endeavour for stem cell biobanking and the development of regenerative therapies. Paravertebral adipose tissue is readily exposed during spinal procedures in children and could be a viable source of stem cells for therapeutic applications. Here, we describe the first case of MSCs isolated from paravertebral adipose tissue (PV-ADMSCs), obtained during a routine spinal surgery on a child. Using quantitative real-time PCR and flow cytometry, we show that PV-ADMSCs have different levels of stem marker expression compared to the MSCs from other sources while having the highest proliferation rate. Furthermore, we evaluate the multipotency of PV-ADMSCs by the three-lineage (adipogenic, osteogenic and chondrogenic) differentiation and compare it to the multipotency of MSCs from other sources. It was found that the PV-ADMSCs have a strong osteogenic potential in particular. Taken together, our data indicate that PV-ADMSCs meet the criteria for successful cell therapy, defined by the International Society for Cellular Therapy (ISCT), and thus, could provide a source of MSCs that is relatively easy to isolate and expand in culture. Due to their strong osteogenic potential, these cells provide a promising basis, especially for orthopaedic applications.
Ključne besede: mesenchymal stem cells, paediatric surgery, paravertebral adipose tissue, regenerative medicine, stem marker expression, osteogenic potential, differentiation
Objavljeno v DKUM: 16.12.2024; Ogledov: 0; Prenosov: 13
Celotno besedilo (3,70 MB)
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Opis: Tissue engineering and regenerative medicine have come a long way in recent decades, but the lack of functioning vasculature is still a major obstacle preventing the development of thicker, physiologically relevant tissue constructs. A large part of this obstacle lies in the development of the vessels on a microscale%the microvasculature%that are crucial for oxygen and nutrient delivery. In this review, we present the state of the art in the field of microvascular tissue engineering and demonstrate the challenges for future research in various sections of the field. Finally, we illustrate the potential strategies for addressing some of those challenges.
Ključne besede: regenerative medicine, tissue engineering, vascularization, biomaterials, microvascularization, coculture, gradients
Objavljeno v DKUM: 18.10.2024; Ogledov: 0; Prenosov: 13
Celotno besedilo (6,81 MB)
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