Iskanje po katalogu digitalne knjižnice

Ključne besede: textiles, metallisation
Objavljeno v DKUM: 11.10.2021; Ogledov: 766; Prenosov: 23
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Opis: Introduction: Although the most common vehicle for transmission of health-care acquired infections is the personto- person transmission route, the role of environment should not be ignored and hospital linen may contribute to the spreading of nosocomial infections. The contact plate method and swabbing are common methods for sampling microorganisms on textiles; however, results are available after two days as they are based on incubation followed by phenotypeidentification. An important alternative is using quick wash-off methods followed by PCR detection, which shortens the identification process from two days to a few hours. Methods: The following test microorganisms at different concentrations were inoculated onto textile swatches and dried overnight: Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and Clostridium difficile. RODAC plate sampling as well as a non-destructive wash-off method for capturing microorganisms from the textilesusing a Morapex device were used. The elution suspension from the Morapex device was used for two methods. In the first method, classical incubation on selective media followed by phenotypic identification was used and in the second method DNA was extracted from the elution suspension followed by amplification and agarose gel electrophoresis to visualize amplified products. Conclusions: All chosen bacteria were found using all methods. However, the most sensitive proved to be detection using PCR amplification as we detected the sample with initial concentration of 102 cfu/mL inoculated onto the textile surface before drying. The final detectablerecovered bacterial concentration on textiles was up to 10 cfu/mL.
Ključne besede: health care associated infections, hospital textiles, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Clostridium difficile, Morapex
Objavljeno v DKUM: 05.04.2017; Ogledov: 1539; Prenosov: 376
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Opis: A hospital environment may act as a significant reservoir for potential pathogens that can be transmitted with hospital textiles, which could represent a source of healthcare-acquired infections. Quantitative assessment of nosocomial pathogens with real time polymerase chain reaction (qPCR) on textiles can serve to verify the achievement of standards for textile hygiene of hospital laundry that assess the risk for acquiring hospital infection frominappropriately disinfected textiles. The aim of the study was to establish qPCR for quantitative assessment of selected common nosocomial pathogens (Clostridium difficile, Staphylococcus aureus, Klebsiella pneumoniaeand Pseudomonas aeruginosa) on hospital textiles and to compare the efficiency of the molecular method to the standard procedures for evaluating the bio burden of textiles in hospitals. This study demonstrated that presenceof nosocomial pathogens on hospital textiles can be confirmed with qPCR even where conventional techniques do not give any results. qPCR offers apossibility to confirm the presence of microorganisms in dead or viable but non-culturable states that cannot be detected by conventional sampling techniques but may still pose a hazard to public health.
Ključne besede: healthcare-acquired infections, hospital textiles, Clostridium difficile, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa
Objavljeno v DKUM: 10.07.2015; Ogledov: 1444; Prenosov: 126
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Opis: The relationships were investigated between the structural and dyeing properties of different structurally modified PA 6 fibers. PA 6 monofilament yarn samples were applied having different crystalline degrees and different content of alpha, respectively gamma crystalline modification. The diffusion coefficients of two different acid dyes were determined together with the content of dyestuff absorbed (under specific conditions) by fiber samples. In order to estimate colorimetry method from the viewpoint of polymer structural change detection, the colors of the dyed PA 6 samples were determined (L*, a*,b*, C*, h coordinates) and the color differences (L*, a*, b*, C*, H*, E*) were calculated between untreated standard and structurally modified samples. The dyeability of PA 6 fibers depended mainly on the way in which crystallinity had been achieved, i.e. on the materialćs history. Different crystalline modifications caused different sorption properties and fiber dyeability. Smaller increases (app 20%) of crystallinity degree caused a decrease in diffusion coefficients. Contrary to expectations, the significant increase of crystallinity degree (app 50%) had an influence on the increase ofdyeability, presumably owing to the formation of larger empty spaces in the structure. The results achieved by colorimetry were in good correlation with dye absorption measurements. The colorimetry of dyed fiber samples was sensitive enough to detect even small differences in the quantities of absorbed dyestuff.
Ključne besede: textiles, PA 6 fibres, dyeing, dyeability, fibre structure, crystallinity, chemical modification
Objavljeno v DKUM: 01.06.2012; Ogledov: 2031; Prenosov: 27
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Opis: This paper presents a research on how to optimize a laundering program to reduce wastewater burden thus achieving a more sustainable laundering procedure. A green surgical textiles program was investigated and the first step of optimization was performed based on the results of measuring certain parameters in the water taken from individual chambers. The wastewater parameters were reduced after optimization of the program although effluent treatment plants for water reuse remain necessary. An experimental laundering procedure with a high dosage of sodium hypochlorite was also conducted in order to observe a correlation between measured values of active chlorine and conductivity. As an approximate correlation was found this could result in thepossibility of incorporating a sensor in the washing machine measuring online the conductivity with the possibility of regulating the dosage of sodium hypochlorite and thus preventing overdosing, which increases the damages of the laundered textiles and the burden of wastewater.
Ključne besede: laundry wastewater, optimization, textiles, sustainable laundering
Objavljeno v DKUM: 01.06.2012; Ogledov: 1711; Prenosov: 92
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