1. Chemo-enzymatic synthesis of viscoelastic pseudopeptidoglycan networksPhilipp Loibner, David Bučak-Gasser, Katharina Schober, Tobias Alexander Steindorfer, Monika Brandtner, Tobias Dorn, Tanja Wrodnigg, Dmytro Neshchadin, Georg Gescheidt-Demner, Matej Bračič, Florian Lackner, Tamilselvan Mohan, Karin Stana-Kleinschek, Rupert Kargl, 2025, izvirni znanstveni članek Opis: Bacterial peptidoglycans (PGs) are essential targets for antibiotics and immune cells. Chemical methods to reproduce PGs semisynthetically are tedious and wasteful. In this work, we describe a new approach to form pseudo-PGs (PPGs) using the protease papain and custom-made peptides conjugated to a glycan. The kinetics of formation is monitored by rheology and 1H NMR. Viscoelastic gels of controlled strength are formed, depending on the temperature and the number of peptide bridges between the glycan chains. We propose that the new method has a high impact on biomaterials research, since it could be used to deliver peptides, test antibiotic efficacy, or investigate human immune cell response. Ključne besede: biomaterials, bacterial peptidoglycans (PGs), chemo-enzymatic synthesis Objavljeno v DKUM: 03.11.2025; Ogledov: 0; Prenosov: 7
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2. Modular flow synthesis of citric acid-coated superparamagnetic iron oxide nanoparticles : preliminary resultsSabina Vohl, Andreja Nemet, Janja Stergar, 2025, izvirni znanstveni članek Opis: Superparamagnetic iron oxide nanoparticles (SPIONs) with sizes below 10 nm are biocompatible and non-toxic, making them promising for biomedical applications. To prevent their agglomeration and enhance their functionality, the nanoparticles were coated with citric acid (CA), which modifies the surface charge, improves dispersion stability, and facilitates biomedical use. In this work, a modular flow-through microreactor system was employed to synthesize and coat the nanoparticles in a single, continuous two-step process. The system enables precise control over temperature and mixing, ensuring uniform reaction conditions and minimizing hot spots. The synthesized Fe3O4 nanoparticles exhibited an average crystallite size of ~5 nm (XRD) and particle sizes of 4–6 nm (TEM). FTIR analysis confirmed the successful surface functionalization with CA, while TGA indicated a coating mass fraction of approximately 4–20 wt%, increasing with higher CA concentration. Zeta potential measurements revealed strong colloidal stability, with values around −35 mV at pH 6.5. Among the tested CA concentrations, the sample with a molar ratio of Fe3O4:CA = 1:0.25 exhibited the most favorable properties, including narrow size distribution and improved dispersion stability. These findings demonstrate that the continuous modular flow approach enables the reproducible synthesis of highly stable, sub-10 nm CA-coated SPIONs, offering promising potential for biomedical applications, particularly as magnetic resonance imaging (MRI) contrast agents. Ključne besede: superparamagnetic iron oxide nanoparticles, citric acid, modular flow microreactor system, continuous synthesis, zeta potential measurements Objavljeno v DKUM: 03.11.2025; Ogledov: 0; Prenosov: 3
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3. Evolutionary game theory use in healthcare : a synthetic knowledge synthesisPeter Kokol, Jernej Završnik, Helena Blažun Vošner, Bojan Žlahtič, 2025, pregledni znanstveni članek Opis: Background: Evolutionary game theory (EGT), originating from Darwinian competition studies, offers a powerful framework for understanding complex healthcare interactions where multiple stakeholders with conflicting interests evolve strategies over time. Unlike traditional game theory, EGT accounts for bounded rationality and strategic evolution through imitation and selection. Aims and objectives: In our study, we use Synthetic Knowledge Synthesis (SKS) that integrates descriptive bibliometrics and bibliometric mapping to systematically analyze the application of EGT in healthcare. The SKS aimed to identify prolific research topics, suitable publishing venues, and productive institutions/countries for collaboration and funding. Data was harvested from the Scopus bibliographic database, encompassing 539 publications from 2000 to June 2025, Results: Production dynamics is revealing an exponential growth in scholarly output since 2019, with peak productivity in 2024. Descriptive bibliometrics showed China as the most prolific country (376 publications), followed by the United States and the United Kingdom. Key institutions are predominantly Chinese, and top journals include PLoS One and Frontiers in Public Health. Funding is primarily from Chinese entities like the National Natural Science Foundation of China. Bibliometric mapping identified five key research themes: game theory in cancer research, evolution game-based simulation of supply management, evolutionary game theory in epidemics, evolutionary games in trustworthy connected public health, and evolutionary games in collaborative governance. Conclusions: Despite EGT’s utility, significant research gaps exist in methodological robustness, data availability, contextual modelling, and interdisciplinary translation. Future research should focus on integrating machine learning, longitudinal data, and explicit ethical frameworks to enhance EGT’s practical application in adaptive, patient-centred healthcare systems Ključne besede: evolutionary games theory, healthcare, complex healthcare systems, synthetic knowledge synthesis, thematic analysis Objavljeno v DKUM: 29.10.2025; Ogledov: 0; Prenosov: 4
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4. The use of AI in software engineering : a synthetic knowledge synthesis of the recent research literaturePeter Kokol, 2024, pregledni znanstveni članek Opis: Artificial intelligence (AI) has witnessed an exponential increase in use in various applications. Recently, the academic community started to research and inject new AI-based approaches
to provide solutions to traditional software-engineering problems. However, a comprehensive and
holistic understanding of the current status needs to be included. To close the above gap, synthetic
knowledge synthesis was used to induce the research landscape of the contemporary research literature on the use of AI in software engineering. The synthesis resulted in 15 research categories and
5 themes—namely, natural language processing in software engineering, use of artificial intelligence
in the management of the software development life cycle, use of machine learning in fault/defect
prediction and effort estimation, employment of deep learning in intelligent software engineering
and code management, and mining software repositories to improve software quality. The most
productive country was China (n = 2042), followed by the United States (n = 1193), India (n = 934),
Germany (n = 445), and Canada (n = 381). A high percentage (n = 47.4%) of papers were funded,
showing the strong interest in this research topic. The convergence of AI and software engineering
can significantly reduce the required resources, improve the quality, enhance the user experience,
and improve the well-being of software developers. Ključne besede: software engineering, artificial intelligence, machine learning, synthetic knowledge synthesis, AI Objavljeno v DKUM: 30.07.2025; Ogledov: 0; Prenosov: 12
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5. The role of correspondence analysis in medical researchBojan Žlahtič, Peter Kokol, Helena Blažun Vošner, Jernej Završnik, 2024, drugi znanstveni članki Opis: Correspondence analysis (CA) is a multivariate statistical and visualization technique. CA is extremely useful in analyzing either two- or multi-way contingency tables, representing some degree of correspondence between columns and rows. The CA results are visualized in easy-to-interpret “bi–plots,” where the proximity of items (values of categorical variables) represents the degree of association between presented items. In other words, items positioned near each other are more associated than those located farther away. Each bi-plot has two dimensions, named during the analysis. The naming of dimensions adds a qualitative aspect to the analysis. Correspondence analysis may support medical professionals in finding answers to many important questions related to health, wellbeing, quality of life, and similar topics in a simpler but more informal way than by using more complex statistical or machine learning approaches. In that way, it can be used for dimension reduction and data simplification, clustering, classification, feature selection, knowledge extraction, visualization of adverse effects, or pattern detection. Ključne besede: public health, medical research, correspondence analysis, synthetic knowledge synthesis, exploratory data analysis, bibliometric Objavljeno v DKUM: 30.07.2025; Ogledov: 0; Prenosov: 1
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6. Machine learning in primary health care : the research landscapeJernej Završnik, Peter Kokol, Bojan Žlahtič, Helena Blažun Vošner, 2025, pregledni znanstveni članek Opis: Background: Artificial intelligence and machine learning are playing crucial roles in digital transformation, aiming to improve the efficiency, effectiveness, equity, and responsiveness of primary health systems and their services. Method: Using synthetic knowledge synthesis and bibliometric and thematic analysis triangulation, we identified the most productive and prolific countries, institutions, funding sponsors, source titles, publications productivity trends, and principal research categories and themes. Results: The United States and the United Kingdom were the most productive countries; Plos One and BJM Open were the most prolific journals; and the National Institutes of Health, USA, and the National Natural Science Foundation of China were the most productive funding sponsors. The publication productivity trend is positive and exponential. The main themes are related to natural language processing in clinical decision-making, primary health care optimization focusing on early diagnosis and screening, improving health-based social determinants, and using chatbots to optimize communications with patients and between health professionals. Conclusions: The use of machine learning in primary health care aims to address the significant global burden of so-called “missed diagnostic opportunities” while minimizing possible adverse effects on patients. Ključne besede: primary health care, machine learning, research landscape, synthetic knowledge synthesis Objavljeno v DKUM: 24.07.2025; Ogledov: 0; Prenosov: 6
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7. Artificial intelligence and pediatrics : synthetic knowledge synthesisJernej Završnik, Peter Kokol, Bojan Žlahtič, Helena Blažun Vošner, 2024, pregledni znanstveni članek Opis: The first publication on the use of artificial intelligence (AI) in pediatrics dates back to
1984. Since then, research on AI in pediatrics has become much more popular, and the number of
publications has largely increased. Consequently, a need for a holistic research landscape enabling
researchers and other interested parties to gain insights into the use of AI in pediatrics has arisen. To
fill this gap, a novel methodology, synthetic knowledge synthesis (SKS), was applied. Using SKS,
we identified the most prolific countries, institutions, source titles, funding agencies, and research
themes and the most frequently used AI algorithms and their applications in pediatrics. The corpus
was extracted from the Scopus (Elsevier, The Netherlands) bibliographic database and analyzed using
VOSViewer, version 1.6.20. Done An exponential growth in the literature was observed in the last
decade. The United States, China, and Canada were the most productive countries. Deep learning was
the most used machine learning algorithm and classification, and natural language processing was
the most popular AI approach. Pneumonia, epilepsy, and asthma were the most targeted pediatric
diagnoses, and prediction and clinical decision making were the most frequent applications. Ključne besede: pediatrics, artificial intelligence, synthetic knowledge synthesis, bibliometrics, machine learning Objavljeno v DKUM: 01.07.2025; Ogledov: 0; Prenosov: 9
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8. Software quality: how much does it matter?Peter Kokol, 2022, pregledni znanstveni članek Opis: Interconnected computers and software systems have become an indispensable part of
people’s lives in the period of digital transformation. Consequently, software quality research is
becoming more and more critical. There have been multiple attempts to synthesise knowledge
gained in software quality research; however, they were focused mainly on single aspects of software
quality and did not structure the knowledge holistically. To fill this gap, we harvested software
quality publications indexed in the Scopus bibliographic database. We analysed them using synthetic
content analysis which is a triangulation of bibliometrics and content analysis. The search resulted in
15,468 publications. The performance bibliometric analysis showed that the production of research
publications relating to software quality is currently following an exponential growth trend and that
the software quality research community is growing. The most productive country was the United
States, followed by China. The synthetic content analysis revealed that the published knowledge
could be structured into six themes, the most important being the themes regarding software quality
improvement by enhancing software engineering, advanced software testing and improved defect
and fault prediction with machine learning and data mining. Ključne besede: software engineering, software quality, knowledge synthesis, bibliometrics, synthetic knowledge synthesis Objavljeno v DKUM: 07.04.2025; Ogledov: 0; Prenosov: 6
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9. The MINLP approach to topology, shape and discrete sizing optimization of trussesSimon Šilih, Zdravko Kravanja, Stojan Kravanja, 2022, izvirni znanstveni članek Opis: The paper presents the Mixed-Integer Non-linear Programming (MINLP) approach to the
synthesis of trusses. The solution of continuous/discrete non-convex and non-linear optimization
problems is discussed with respect to the simultaneous topology, shape and discrete sizing optimization of trusses. A truss MINLP superstructure of different topology and design alternatives
has been generated, and a special MINLP model formulation for trusses has been developed. In the
optimization model, a mass objective function of the structure has been defined and subjected to
design, load and dimensioning constraints. The MINLP problems are solved using the Modified
Outer-Approximation/Equality-Relaxation (OA/ER) algorithm. Multi-level MINLP strategies are introduced to accelerate the convergence of the algorithm. The Modified Two-Phase and the Sequential
Two-Phase MINLP strategies are proposed in order to solve highly combinatorial topology, shape
and discrete sizing optimization problems. The importance of local buckling constraints on topology
optimization is also discussed. Some simple numerical examples are shown at the end of the paper to
demonstrate the suitability and efficiency of the proposed method. Ključne besede: structural synthesis, topology optimization, discrete sizing optimization, mixed-integer non-linear programming, MINLP, modified OA/ER algorithm, multi-level MINLP strategies, steel structures, trusses Objavljeno v DKUM: 11.03.2025; Ogledov: 0; Prenosov: 12
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10. Synergic effect of large MXene nanosheets and protective coatings on improved electroconductivity and wash durability of MXene/polymer-modified cotton fabricLaura Jug, Silvo Hribernik, Alenka Ojstršek, 2025, izvirni znanstveni članek Opis: E-textiles and their wearable analogues are finding applications in a myriad of sectors, ranging from sensors to health and sports applications. The development of a truly functional and reusable textile substrate presents a challenging task; its design encompasses the fabrication of optimal functional conductive particles, as well as devising strategies for their application that will ensure their functional properties (e.g. conductivity) are retained in an undiminished state for a foreseeable period. In the presented study, we tackled these two aspects in an interdependent way: i) The enhancement of the electrical conductivity of MXene-modified cotton fabric by increasing the lateral size of nanosheets during the Ti3C2TX synthesis, and ii) The improvement of washing durability of MXenes on the fabric surface by selecting suitable protective coatings. The results of Scanning Electron Microscopy (SEM), X-ray powder Diffraction (XRD), Dynamic Light Scattering (DLS) and Atomic Force Microscopy (AFM) revealed the successful synthesis of large and stable MXene nanosheets with ultrathin flakelike nanostructures, high colloidal stability and delamination yields. Using multiple application procedures of dipping and drying, the MXene nanosheets formed extensive adhesion areas on the cotton fabric and overlapped the fibre pores, thus reducing the interfacial resistance between the sheets and improving the coating uniformity and, consequently, increasing electrical conductivity. Weaker adhesion and depletion of large nanosheets were further effectively prevented by protective polymer coatings. The MXene-coated/protected fabrics had sufficient electrical conductivity, even after 20 laundering cycles. Moreover, the surface hydrophobicity was negligibly reduced, preventing water accessibility and, thus, increasing the oxidation stability of the applied MXenes. Ključne besede: Ti3C2TX MXene, synthesis parameters, cotton fabric, electrical conductivity, protective coatings, washing durability Objavljeno v DKUM: 10.03.2025; Ogledov: 0; Prenosov: 14
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