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Effect of base sequence on G-wire formation in solution
Lea Spindler, Martin Rigler, Irena Drevenšek Olenik, Mateus Webba da Silva, Nason Ma'ani Hessari, 2010, izvirni znanstveni članek

Opis: The formation and dimensions of G-wires by different short G-rich DNA sequences in solution were investigated by dynamic light scattering (DLS) and polyacrilamide gel electrophoresis (PAGE). To explore the basic principles of wire formation, we studied the effects of base sequence, method of preparation, temperature, and oligonucleotide concentration. Both DLS and PAGE show that thermal annealing induces much less macromolecular self-assembly than dialysis. The degree of assembly and consequently length of G-wires (5-6 nm) are well resolved by both methods for DNA sequences with intermediate length, while some discrepancies appear for the shortest and longest sequences. As expected, the longest DNA sequence gives the longest macromolecular aggregates with a length of about 11 nm as estimated by DLS. The quadruplex topologies show no concentration dependence in the investigated DNA concentration range (0.1 mM–0.4 mM) and no structural change upon heating.
Ključne besede: DNA, dynamic light scattering, DLS, polyacrylamide gel electrophoresis, PAGE, genetics
Objavljeno: 14.06.2017; Ogledov: 164; Prenosov: 16
.pdf Celotno besedilo (3,34 MB)

Cross-linked porous poly(acrylic acid-co-acrylamide) from high internal phase emulsions: preparation and functionalisation
Janja Majer, Peter Krajnc, 2009, izvirni znanstveni članek

Opis: By using emulsions with high volume fractions of internal phase as polymerisation media porous copolymers of acrylic acid and acrylamide, cross-linked by methylenebisacrylamide, were prepared. The ratio of acrylic acid to acrylamide were varied (molar ratios 70:30, 50:50, 30:70) in order to yield polymers with various loading of acidic functional groups. Porous polymers with an open-cellular architecture were obtained with void diameters between 2.8 μm and 3.9 μm and with interconnecting pores approx. 0.3 μm in diameter. The chemical composition of the polymers influenced the conversion degrees during the process of functionalisation of acidic groups to acid chloride, being the highest in the case of polymer with the lowest content of acrilyc acid.
Ključne besede: porous monoliths, copolymers, polyacrylic acid, polyacrylamide, plyHIPE, high internal phase emulsion
Objavljeno: 17.08.2017; Ogledov: 228; Prenosov: 27
.pdf Celotno besedilo (218,85 KB)

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