1. Pig weight estimation according to RGB image analysisAndras Kárpinszky, Gergely Dobsinszki, 2023, izvirni znanstveni članek Opis: In pig farming, knowing the exact weight of each animal is critical for the owner. Such information can help determine the amount and type of feed that needs to be fed to a specific fattening pig. Weighing pigs has always been problematic, because it is highly time consuming, and herding the pigs on the scale is extremely cumbersome. Moreover, it causes stress to the animals. The aim of our study was to build an RGB-based system that could estimate the daily weight of pigs and individual animal weight. The study was set up in a 100-day rotation in a commercial pig farm where we monitored 32 pigs. We developed a system to identify the features of the pigs, more particularly the head, shoulder, belly, and rump part. Three different models
were tested, and their main differences were linked to image processing and training data. Using these models, we received higher than 97% accuracy between the predicted and the manually recorded weight of the animals. This system allows owners to manage and monitor their pigs using our web interface, allowing them to make crucial decisions during the farming process. Ključne besede: image processing, pig size, decision support system, precision livestock farming Objavljeno v DKUM: 25.04.2025; Ogledov: 0; Prenosov: 2
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2. Suckling behaviour of piglets affected by body weight and sexMartina Dobaj Gomboc, Janko Skok, Dejan Škorjanc, Maja Prevolnik Povše, 2023, izvirni znanstveni članek Opis: Piglet suckling behaviour has been extensively studied, but surprisingly, there are not many studies that specifically consider body weight and sex in this context. These two basic individual characteristics have been considered more as supporting data but not as main factors. Therefore, the objective of the present research was to examine the effect body weight on suckling behaviour of piglets during lactation separately by sex. The study comprised 14 litters with a total of 158 piglets (85 male, 73 female, litter size 6−15 piglets). Suckling behaviour was described in terms of suckling position (the teat at which the piglet suckled during suckling), suckling territory (the range two outermost suckling positions of the piglet encompasses) and suckling
stability (the tendency to suckle successively at the same position), and was observed in six periods: 0−3, 4−7, 8−10, 11−14, 15−21 and 22−32 days of age. In each period, piglets were also weighed. The udder was divided into three areas: anterior (1st-2nd teat pair), middle (3rd-5th teat pair) and posterior (6th-8th teat pair). Body weight affected suckling behaviour differently in male and female piglets (i.e., all traits in females, suckling stability only in males). The relative body weight of females decreased significantly from the anterior towards the posterior part. Heavy females (but not males) suckled considerably more frequently on the anterior area. Heavier piglets (male and female) established more stable suckling order. Suckling territory of males was quite large, but did not differ among body weight classes. Interestingly, light females visited significantly larger suckling territory than heavy females. The present results fill a gap in the otherwise broad knowledge of pig suckling behaviour, which is of great importance for litter management during lactation, especially when cross-fostering is implemented. In this context, knowledge of the detailed role of sex and body weight is of particular importance because breeders rely mainly on basic body traits when managing litters. Ključne besede: pig, lactation, body weight, sex, suckling position, suckling stability, suckling territory Objavljeno v DKUM: 25.04.2025; Ogledov: 0; Prenosov: 3
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3. Enrichments adjusted to the number of litters: simple approach to improve the welfare of weaned piglets under farm conditionsNejc Kuri, Janko Skok, Dejan Škorjanc, Maja Prevolnik Povše, 2024, izvirni znanstveni članek Ključne besede: pig, weaning, welfare, aggresion, behaviour, injuries, environmental enrichment Objavljeno v DKUM: 16.04.2025; Ogledov: 0; Prenosov: 13
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4. Lipid deposition and metabolism in local and modern pig breeds : a reviewKlavdija Poklukar Žnidaršič, Marjeta Čandek-Potokar, Nina Batorek Lukač, Urška Tomažin, Martin Škrlep, 2020, pregledni znanstveni članek Opis: Modern pig breeds, which have been genetically improved to achieve fast growth and a lean meat deposition, differ from local pig breeds with respect to fat deposition, fat specific metabolic characteristics and various other properties. The present review aimed to elucidate the mechanisms underlying the differences between fatty local and modern lean pig breeds in adipose tissue deposition and lipid metabolism, taking into consideration morphological, cellular, biochemical, transcriptomic and proteomic perspectives. Compared to modern breeds, local pig breeds accumulate larger amounts of fat, which generally contains more monounsaturated and saturated fatty acids; they exhibit a higher adipocyte size and higher activity of lipogenic enzymes. Studies using transcriptomic and proteomic approaches highlighted several processes like immune response, fatty-acid turn-over, oxidoreductase activity, mitochondrial function, etc. which differ between local and modern pig breeds. Ključne besede: pig, adipose tissue, local breeds, modern breeds, cellularity, transcriptome, proteom, adaptation Objavljeno v DKUM: 06.02.2025; Ogledov: 0; Prenosov: 7
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5. Effect of Lactobacillus spp. on adhesion, invasion, and translocation of Campylobacter jejuni in chicken and pig small-intestinal epithelial cell linesMaja Šikić Pogačar, Tomaž Langerholc, Dušanka Mičetić-Turk, Sonja Smole Možina, Anja Klančnik, 2020, izvirni znanstveni članek Opis: Background : Campylobacter spp. are a major cause of bacterial food-borne diarrhoeal disease. This mainly arises through contamination of meat products during processing. For infection, Campylobacter spp. must adhere to epithelial cells of the mucus layer, survive conditions of the gastrointestinal tract, and colonise the intestine of the host. Addition of probiotic bacteria might promote competitive a dhesion to epithelial cells, consequently reducing Campylobacter jejuni colonisation. Effect of Lactobacillus spp. (PCS20, PCS22, PCS25, LGG, PCK9) on C. jejuni adhesion, invasion and translocation in pig (PSI cl.1) and chicken (B1OXI) small-intestine cell lines, as well as pig enterocytes (CLAB) was investigated.
Results : Overall, in competitive adhesion assays with PSI cl.1 and CLAB cell monolayers, the addition of Lactobacillus spp. reduced C. jejuni adherence to the cell surface, and negatively affected the C. jejuni invasion. Interestingly, Lactobacillus spp. significantly impaired C. jejuni adhesion in three-dimensional functional PSI cl.1 and B1OXI cell models. Also, C. jejuni did not translocate across PSI cl.1 and B1OXI cell monolayers when co-incubated with probiotics. Among selected probiotics, Lactobacillus rhamnosus LGG was the strain that reduced adhesion efficacy of C. jejuni most significantly under co-culture conditions.
Conclusion : The addition of Lactobacillus spp. to feed additives in livestock nutrition might be an effective novel strategy that targets Campylobacter adhesion to epithelial cells, and thus prevents colonisation, reduces the transmission, and finally lowers the incidence of human campylobacteriosis. Ključne besede: Campylobacter jejuni, Lactobacillus spp., chicken and pig cell line, adhesion, invasion, translocation Objavljeno v DKUM: 06.02.2025; Ogledov: 0; Prenosov: 7
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6. The effect of supplementing pig diet with chestnut wood extract or hops on fresh meat and dry-cured productsUrška Tomažin, Martin Škrlep, Maja Prevolnik Povše, Nina Batorek Lukač, Danijel Karolyi, Matjaž Červek, Marjeta Čandek-Potokar, 2020, izvirni znanstveni članek Opis: Oxidation is one of the major reasons for impaired quality of meat and meat products but can be prevented by the addition of antioxidants. In the present study, the effect of dietary sweet chestnut wood extract and hop cones on the quality and oxidative stability of meat and dry-cured products was investigated. Control pigs (N = 11) were fed a commercial diet (13.1 MJ metabolizable energy, 15.5% crude protein), while the other two experimental groups were supplemented with 3% of sweet chestnut wood extract (Tannin; N = 12) or 0.4% of hop cones (Hops; N = 11). The quality of meat and dry-cured products was evaluated by means of chemical composition, water holding capacity, objective color, and lipid and protein oxidation. No major effects of sweet chestnut wood extract or of hops supplementation were observed, nevertheless, some indications of improved water holding capacity could be attributed to antioxidants supplementation. The color evolution of dry-cured bellies from Tannin and Hops groups of pigs during refrigerated storage was also indicative of an improved oxidative stability. Ključne besede: pig meat, meat quality, pig diet, tannin extract, hops, dry-cured products, oxidation, pigs Objavljeno v DKUM: 23.12.2024; Ogledov: 0; Prenosov: 8
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7. Agonistic interactions between littermates reappear after mixing multiple litters at weaning in pigsMaja Prevolnik Povše, Nikolina Mesarec, Janko Skok, Dejan Škorjanc, 2021, izvirni znanstveni članek Opis: Weaning involves mixing of unfamiliar litters and is accompanied by an outbreak of aggression, which is usually attributed to between-litter (BL) interactions. In the present study, we thus focused on post-weaning agonism (fighting and mounting) between littermates (within-litter, WL). Two litters were weaned into two pens separated by an empty pen and connected by narrow passages that were opened 24 h after weaning. WL interactions accounted for 38% and 68% of all fights and mounts, respectively, during a 7-day experimental period. After the passages were opened, not only BL, but also WL interactions increased significantly (by 6- to 7-fold on the first day of mixing). WL fights then gradually decreased, while WL mounts continued to increase. During the experimental period, the proportion of both WL fights and mounts decreased. The majority of WL fights (%80%) and mounts (%65%) occurred in home pens. A significantly higher percentage of draws was found in WL fights (50% of initiator wins and 41% of draws) compared to BL fights (80% of initiator wins and 11% of draws). Results also showed less asymmetry in the body weight of piglets involved in WL interactions. Mixing of litters at weaning was shown to involve not only intense interactions between unfamiliar piglets but also the recurrence of agonistic interactions between littermates, which is something we should keep in mind when pursuing the highest standards of weaner welfare. Ključne besede: pig, weaning, within-litter interactions, fighting, mounting Objavljeno v DKUM: 06.12.2024; Ogledov: 0; Prenosov: 15
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8. DigiPig : First developments of an automated monitoring system for body, head and tail detection in intensive pig farmingMarko Ocepek, Anja Žnidar, Miha Lavrič, Dejan Škorjanc, Inger Lise Andersen, 2022, izvirni znanstveni članek Opis: The goal of this study was to develop an automated monitoring system for the detection of pigs’ bodies, heads and tails. The aim in the first part of the study was to recognize individual pigs (in lying and standing positions) in groups and their body parts (head/ears, and tail) by using machine learning algorithms (feature pyramid network). In the second part of the study, the goal was to improve the detection of tail posture (tail straight and curled) during activity (standing/moving around) by the use of neural network analysis (YOLOv4). Our dataset (n = 583 images, 7579 pig posture) was annotated in Labelbox from 2D video recordings of groups (n = 12–15) of weaned pigs. The model recognized each individual pig’s body with a precision of 96% related to threshold intersection over union (IoU), whilst the precision for tails was 77% and for heads this was 66%, thereby already achieving human-level precision. The precision of pig detection in groups was the highest, while head and tail detection precision were lower. As the first study was relatively time-consuming, in the second part of the study, we performed a YOLOv4 neural network analysis using 30 annotated images of our dataset for detecting straight and curled tails. With this model, we were able to recognize tail postures with a high level of precision (90%). Ključne besede: pig, welfare, image processing, object detection, deep learning, smart farming Objavljeno v DKUM: 11.07.2024; Ogledov: 87; Prenosov: 9
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9. Ensiling, in vitro rumen digestion and soaking in slurry altered the germination capacity of Rumex obtusifolius seedsAnastazija Gselman, Maksimiljan Brus, 2023, izvirni znanstveni članek Opis: This study investigated whether the process of ensiling and in vitro digestion in rumen juice, as well as the response to soaking in pig or cattle slurry, affects the germination rate and germination energy of Rumex obtusifolius (broad-leaved dock) seeds. Seeds were subjected to different treatments (200 seeds each) in three experiments: (I) seed ensiling (8 weeks) followed by in vitro rumen digestion (24, 36 and 48 h); (II) the soaking of non-ensiled and ensiled seeds in cattle or pig slurry (2, 4 and 24 weeks); and (III) the in vitro rumen digestion (24, 36 and 48 h) of non-ensiled and ensiled seeds followed by soaking in cattle or pig slurry (24 weeks). The control treatment included untreated seed (0—non-ensiled seed; 0—no in vitro rumen digestion; and 0—no soaking in slurry). Germination tests (germination rate and germination energy) were then conducted in four replicates in the germination chamber under alternating day (20–35 °C for 14 h under light) and night conditions (17–20 °C for 10 h without light) at 75% relative humidity. Experiment I showed that ensiling significantly (p ≤ 0.001) reduced both the germination rate and germination energy of R. obtusifolius seeds. In addition, the length of in vitro digestion duration that the non-ensiled seeds were subjected to significantly (p ≤ 0.001) reduced their germination energy but not the total germination rate. However, the seeds that were subjected to the process of ensiling and in vitro digestion in the rumen lost their germination completely. The Experiment II investigated the effects of soaking non-ensiled seeds in slurry and showed that germination rates were comparable in pig and cattle slurry. Longer soaking times significantly reduced the germination rate, with no germination observed after 24 weeks. The Experiment III considered the combined effects of in vitro digestion and slurry soaking and showed that rumen digestion reduced the proportion of germinable seeds. Germination was inhibited in pig slurry, while in cattle slurry, a decreasing germination rate was observed with increasing digestion time. Ključne besede: broad-leaved dock, in vitro rumen digestion, ensiling, pig and cattle slurry, seed germination Objavljeno v DKUM: 05.04.2024; Ogledov: 308; Prenosov: 19
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10. The Digital Pig: Automatic Systems for Behavior Detection in Weaned PigsAnja Lešnik, 2020, diplomsko delo Opis: In this bachelor's thesis, we used machine learning techniques to detect pigs in group pens, which would help to improve the welfare and comfort of pigs. Mask-RCNN was used for object segmentation. The implementation was based on Resnet101. The goal was to achieve the highest possible precision in detection of the pig's body, head, and tail. We predicted that the accuracy will be the highest for body detection and lower for head and tail detection. We also concluded that the difference in precision and recall will be less than 10% between hand-labeled bounding boxes and the predicted bounding boxes from our model. As predicted, body detection represented the highest results, as the accuracy of head and tail detection was lower. The difference between precision and recall was 10% for body detection and higher than 10% for head and tail detection. Precision of the body detection was 96%, as the whole body is easier to detect. The head detection precision score was 66%. Tail detection precision was 77%, which is a large difference compared to the percentage of head detection. The use of machine learning in livestock farming could be a potentially useful tool for detecting welfare in pigs, as it would reduce the frequency of aggressive behaviors and the number of injuries. In the future, we want to refine our model to achieve higher precision for head and tail detection. Once the algorithm has clearly detected all the pigs in the image, we will try to refine the model to detect different forms of behavior. This technology would help us to evaluate welfare, which would be improved if necessary. Ključne besede: pig, pig annotation, behavior, welfare, machine learning Objavljeno v DKUM: 08.09.2020; Ogledov: 1051; Prenosov: 188
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