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Comparison of the esterification of fructose and palmitic acid in organic solvent and supercritical carbon dioxide
Saša Šabeder, Maja Leitgeb, Željko Knez, 2005, izvirni znanstveni članek

Opis: Sugar fatty acid esters are nonionic surfactants, which are used for personal care products, cosmetic applications, and as emulsifiers for food. In recent years, enzymatic synthesis of sugar fatty acid esters is attracting keen attention as a new manufacturing method for future application. Reaction parameters of lipase-catalyzed synthesis of fructose fatty acid esters in organic solvent were optimized in a batch reactor at atmospheric pressure. Optimum conditions for reaction performed in organic solvent at atmospheric pressure were 10% (wžw of substrates) of lipase from Candida antarctica B, 12.14% (wžw of reaction mixture) of molecular sieves at 60 C, and stirring rate of 600 rpm. Esterification of fructose and palmitic acid was performed insupercritical carbon dioxide with and without addition of cosolvent (organicsolvent) at 60 C. Effect of pressure on enzyme activity was studied.
Ključne besede: chemical processing, high pressure technology, supercritical CO2, esterification, organic solvent, lipase catalysed synthesis, fructose fatty esters, Candida antarctica B, enzyme activity
Objavljeno v DKUM: 01.06.2012; Ogledov: 2400; Prenosov: 186
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Enzymatic synthesis of sugar fatty acid esters in organic solvent and in supercritical carbon dioxide and their antimicrobial activity
Maja Leitgeb, Saša Šabeder, Željko Knez, 2008, izvirni znanstveni članek

Opis: Lipase-catalyzed synthesis of different sugar fatty acid esters was performed in high yields in 2-methyl-2-butanol at atmospheric pressure and in supercritical carbon dioxide (SC CO2) at 10 MPa. Influence of molecular sievesconcentration on conversion in SC CO2 was studied. Growth inhibitory effect of commercial sucrose fatty acid esters and enzymatically synthesized sucrose and fructose fatty acid esters on Gram-positive and Gram-negative micro-organisms, as well as on yeast was tested. Sucrose laurate inhibited the growth of Bacillus cereus food poisoning bacteria at a concentration of 9.375 mg/ml.
Ključne besede: biocatalysis, lipase, sugar fatty acid ester, organic solvent, cupercritical CO2, antimicrobial activity
Objavljeno v DKUM: 31.05.2012; Ogledov: 2326; Prenosov: 108
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