1. Using machine learning and natural language processing for unveiling similarities between microbial dataLucija Brezočnik, Tanja Žlender, Maja Rupnik, Vili Podgorelec, 2024, izvirni znanstveni članek Opis: Microbiota analysis can provide valuable insights in various fields, including diet and nutrition, understanding health and disease, and in environmental contexts, such as understanding the role of microorganisms in different ecosystems. Based on the results, we can provide targeted therapies, personalized medicine, or detect environmental contaminants. In our research, we examined the gut microbiota of 16 animal taxa, including humans, as well as the microbiota of cattle and pig manure, where we focused on 16S rRNA V3-V4 hypervariable regions. Analyzing these regions is common in microbiome studies but can be challenging since the results are high-dimensional. Thus, we utilized machine learning techniques and demonstrated their applicability in processing microbial sequence data. Moreover, we showed that techniques commonly employed in natural language processing can be adapted for analyzing microbial text vectors. We obtained the latter through frequency analyses and utilized the proposed hierarchical clustering method over them. All steps in this study were gathered in a proposed microbial sequence data processing pipeline. The results demonstrate that we not only found similarities between samples but also sorted groups’ samples into semantically related clusters. We also tested our method against other known algorithms like the Kmeans and Spectral Clustering algorithms using clustering evaluation metrics. The results demonstrate the superiority of the proposed method over them. Moreover, the proposed microbial sequence data pipeline can be utilized for different types of microbiota, such as oral, gut, and skin, demonstrating its reusability and robustness.
Ključne besede: machine learning, NLP, hierarchical clustering, microbial data, microbiome, n-grame
Objavljeno v DKUM: 04.09.2024; Ogledov: 38; Prenosov: 9
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2. Gut microbiome composition in patients with chronic urticaria : a review of current evidence and dataMirela Kristo, Liborija Lugović-Mihić, Melba Munoz, Maja Rupnik, Aleksander Mahnič, Petar Ozretić, Morana Jaganjac, Diana Cesić, Matea Kuna, 2023, pregledni znanstveni članek Opis: Recent studies have linked gut microorganism composition and chronic urticaria (CU); however, the underlying mechanisms responsible for this connection are unknown. Since the human immune system is in homeostasis with microbiota, and the composition of the microbiome regulates the development and function of the immune system, it is likely that an alteration of microbiota components (a dysbiosis) could influence the course of chronic spontaneous urticaria (CSU), including disease severity, patient quality of life and treatment outcome. To date, several studies have identified changes in the gut microbiota composition of patients with CSU, though only a few have exhibited metabolic abnormalities associated with gut dysbiosis. The studies on CSU patients predominantly showed that the relative abundance of beneficial bacteria was decreased (Firmicutes and Bacteroides), while that of opportunistic bacteria was increased (Enterobacteria and Proteobacteria). In addition, serum metabolome analysis revealed that gut microbiota-associated alterations in unsaturated fatty acids and the butanoate metabolism pathway may play a role in CSU. These findings are potentially associated with inflammation mediated by the imbalance of Th1/Th2/Th17 cytokines, which might contribute to CSU pathogenesis. Further research in this field could improve clinical, diagnostic, and therapeutic approaches to patients with CSU. By applying new knowledge on gut microbial communities and metabolomics, future CSU therapies could modify the microbiota composition using agents such as probiotics or other similar agents, which, in combination with current standard therapies, could hopefully lead to a reduction in symptoms and an improved quality of life for CSU patients.Recent studies have linked gut microorganism composition and chronic urticaria (CU); however, the underlying mechanisms responsible for this connection are unknown. Since the human immune system is in homeostasis with microbiota, and the composition of the microbiome regulates the development and function of the immune system, it is likely that an alteration of microbiota components (a dysbiosis) could influence the course of chronic spontaneous urticaria (CSU), including disease severity, patient quality of life and treatment outcome. To date, several studies have identified changes in the gut microbiota composition of patients with CSU, though only a few have exhibited metabolic abnormalities associated with gut dysbiosis. The studies on CSU patients predominantly showed that the relative abundance of beneficial bacteria was decreased (Firmicutes and Bacteroides), while that of opportunistic bacteria was increased (Enterobacteria and Proteobacteria). In addition, serum metabolome analysis revealed that gut microbiota-associated alterations in unsaturated fatty acids and the butanoate metabolism pathway may play a role in CSU. These findings are potentially associated with inflammation mediated by the imbalance of Th1/Th2/Th17 cytokines, which might contribute to CSU pathogenesis. Further research in this field could improve clinical, diagnostic, and therapeutic approaches to patients with CSU. By applying new knowledge on gut microbial communities and metabolomics, future CSU therapies could modify the microbiota composition using agents such as probiotics or other similar agents, which, in combination with current standard therapies, could hopefully lead to a reduction in symptoms and an improved quality of life for CSU patients.
Ključne besede: gut microbiome, intestinal microbiome composition, chronic spontaneous urticaria, dysbiosis, metabolome
Objavljeno v DKUM: 19.02.2024; Ogledov: 360; Prenosov: 15
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3. Relationship between genome and epigenome - challenges and requirements for future researchGeneviève Almouzni, Neža Grgurevič, Gregor Majdič, Uroš Potočnik, Janez Ilaš, 2014, drugi znanstveni članki Opis: Understanding the links between genetic, epigenetic and non-genetic factors throughout the lifespan and across generations and their role in disease susceptibility and disease progression offer entirely new avenues and solutions to major problems in our society. To overcome the numerous challenges, we have come up with nine major conclusions to set the vision for future policies and research agendas at the European level.
Ključne besede: genome, epigenome, microbiome, environment
Objavljeno v DKUM: 28.06.2017; Ogledov: 1168; Prenosov: 372
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4. A co-culture model of the developing small intestine offers new insight in the early immunomodulation of enterocytes and macrophages by Lactobacillus spp. through STAT1 and NF-kB p65 translocationMartin Trapečar, Aleš Goropevšek, Mario Gorenjak, Lidija Gradišnik, Marjan Rupnik, 2014, izvirni znanstveni članek Opis: The early establishment of a complete microbiome has been shown to play an integral part in the development and maintenance of an intact intestine and its immune system, although much remains unknown about the specific mechanisms of immune modulation in newborns. In our study we show in a co-culture model of the undeveloped small intestine that members of Lactobacillus spp. influence STAT1 and NF-kB p65 nuclear translocation in both intestinal epithelial cells as well as underlying macrophages. Moreover, by using imaging flow cytometry we were able to monitor each individual cell and create a framework of the percentage of cells in which translocation occurred in challenged versus control cell populations. We also observed a significant difference in baseline translocation in intestinal cells when cultured alone versus those in a co-culture model, underpinning the importance of 3D models over monolayer set-ups in epithelial in vitro research. In conclusion, our work offers new insights into the potential routes by which the commensal microbiome primes the early immune system to fight pathogens, and shows how strain-specific these mechanisms really are.
Ključne besede: microbiome, Lactobacillus, immune system, pathogens
Objavljeno v DKUM: 19.06.2017; Ogledov: 1485; Prenosov: 377
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