1. Mesenchymal stem cells isolated from paediatric paravertebral adipose tissue show strong osteogenic potentialJan Rožanc, Lidija Gradišnik, Tomaž Velnar, Minja Gregorič, Marko Milojević, Boštjan Vihar, Boris Gole, Uroš Maver, 2022, izvirni znanstveni članek Opis: Mesenchymal stem cells (MSCs) represent the basis of novel clinical concepts in cellular therapy and tissue regeneration. Therefore, the isolation of MSCs from various tissues has become an important endeavour for stem cell biobanking and the development of regenerative therapies. Paravertebral adipose tissue is readily exposed during spinal procedures in children and could be a viable source of stem cells for therapeutic applications. Here, we describe the first case of MSCs isolated from paravertebral adipose tissue (PV-ADMSCs), obtained during a routine spinal surgery on a child. Using quantitative real-time PCR and flow cytometry, we show that PV-ADMSCs have different levels of stem marker expression compared to the MSCs from other sources while having the highest proliferation rate. Furthermore, we evaluate the multipotency of PV-ADMSCs by the three-lineage (adipogenic, osteogenic and chondrogenic) differentiation and compare it to the multipotency of MSCs from other sources. It was found that the PV-ADMSCs have a strong osteogenic potential in particular. Taken together, our data indicate that PV-ADMSCs meet the criteria for successful cell therapy, defined by the International Society for Cellular Therapy (ISCT), and thus, could provide a source of MSCs that is relatively easy to isolate and expand in culture. Due to their strong osteogenic potential, these cells provide a promising basis, especially for orthopaedic applications.
Ključne besede: mesenchymal stem cells, paediatric surgery, paravertebral adipose tissue, regenerative medicine, stem marker expression, osteogenic potential, differentiation
Objavljeno v DKUM: 16.12.2024; Ogledov: 0; Prenosov: 5
 Celotno besedilo (3,70 MB)
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2. Comparison of canine and feline adipose-derived mesenchymal stem cells/medicinal signaling cells with regard to cell surface marker expression, viability, proliferation, and differentiation potentialMetka Voga, Valerija Kovač, Gregor Majdič, 2021, izvirni znanstveni članek Opis: Remarkable immunomodulatory abilities of mesenchymal stem cells, also called multipotent mesenchymal stromal cells or medicinal signaling cells (MSCs), have entailed significant advances in veterinary regenerative medicine in recent years. Despite positive outcomes from MSC therapies in various diseases in dogs and cats, differences in MSC characteristics between small animal veterinary patients are not well-known. We performed a comparative study of cells' surface marker expression, viability, proliferation, and differentiation capacity of adipose-derived MSCs (ADMSCs) from dogs and domestic cats. The same growth media and methods were used to isolate, characterize, and culture canine and feline ADMSCs. Adipose tissue was collected from 11 dogs and 8 cats of both sexes. The expression of surface markers CD44, CD90, and CD34 was detected by flow cytometry. Viability at passage 3 was measured with the hemocytometer and compared to the viability measured by flow cytometry after 1 day of handling. The proliferation potential of MSCs was measured by calculating cell doubling and cell doubling time from second to eighth passage. Differentiation potential was determined at early and late passages by inducing cells toward adipogenic, osteogenic, and chondrogenic differentiation using commercial media. Our study shows that the percentage of CD44+CD90+ and CD34−/− cells is higher in cells from dogs than in cells from cats. The viability of cells measured by two different methods at passage 3 differed between the species, and finally, canine ADMSCs possess greater proliferation and differentiation potential in comparison to the feline ADMSCs.
Ključne besede: mesenchymal stem cells, dog, cat, comparison, proliferation, differentiation, cell surface marker, viability
Objavljeno v DKUM: 21.10.2024; Ogledov: 0; Prenosov: 6
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3. Arhitekturna zasnova razvojno multimodalnega centra na industrijskem območju občine Hoče-Slivnica : magistrsko deloJan Ekart, 2021, magistrsko delo Opis: Tema magistrskega dela izhaja iz problematike širšega območja občine Hoče-Slivnica, natančneje predela umeščenega sredi infras- trukturnega trikotnika avtocestnega razcepa Slivnica, letališča Ed- varda Rusjana, objekta Magna in industrijske cone južnega Mari- bora.
Zasnovana je arhitekturna rešitev, ki na novo definira območje obeh gramoznic in v prostoru ustvarja novo stičiščno točko, ki de- luje kot možgani širšega industrijskega in podjetniškega območja. Površine, ki jih objekt zavzame, jih tudi vrne v obliki uporabne, ze- lene strehe.
Glavna tema naloge je zasnova podjetniškega in raziskovalnega huba, ki se razteza po celotnem objektu. Programsko je objekt zas- novan fleksibilno. Cilj je novim ali obstoječim velikim podjetjem v regiji omogočiti vse potrebne prostore za delo, bivanje in preživl- janje prostega časa, hkrati pa omogočati programe in aktivnosti, ki spodbujajo hitrejšo rast poslovanja.
Forma objekta izhaja iz osnovnih prostorskih linij in osi, ki so bile zaznane v analizah, hkrati pa iz potrebe po subtilnem prostorskem markerju in posnemanju narave oziroma vpenjanje arhitekture vanjo.
Ključne besede: multimodalni center, Hoče-Slivnica, marker, regeneracija, industrijska cona
Objavljeno v DKUM: 24.12.2021; Ogledov: 1092; Prenosov: 194
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4. Določitev optimalnega markerja homogenosti krmnih mešanic : magistrsko deloKristina Gluhak, 2019, magistrsko delo Opis: Krmne mešanice so sestavljene iz organskih in anorganskih materialov. Zakonodaja zahteva, da so zmesi krmnih mešanic po sestavi homogene in da posamezne komponente med zmesmi ne povzročajo kontaminacije krmnih mešanic med sabo, zato je potrebno izvesti testa homogenosti in navzkrižne kontaminacije. Kot markerji se lahko uporabljajo posamezna barvila (na primer metilvijolično), antibiotiki, kovine, magnetni delci itd.
Osnovni namen raziskav v okviru magistarskega dela je razvoj analizne metode, ki bo po statistični obdelavi rezultatov omogočala kvantitativno določanje markerja v različnih krmnih mešanicah. Proučevali smo manganov(II) oksid (MnO) kot možni marker. V vzorcih koruze in krmne mešanice smo analizirali vsebnost petih kovin: bakra, kalija, mangana, fosforja in cinka. Vsebnost kovin smo določili z induktivno sklopljeno plazmo z optično emisijsko spektrometrijo (ICP-OES). Pridobljene rezultate kemijske analize smo komometrično obdelali. Izbrane kovine smo analizirali na vzorcih podjetja Perutnina Ptuj d.d.
Izvedli smo validacijo analizne metode in ovrednotili merilno negotovost. Ugotovili smo, da je metoda določanja izbranih kovin v koruzi in krmni mešanici z ICP-OES linearna, natančna, točna in primerna za ovrednotenje vsebnosti izbranih kovin v navedenih vzorcih.
Ključne besede: ICP-OES, homogenost, koruza, krma, marker, validacija, merilna negotovost
Objavljeno v DKUM: 10.10.2019; Ogledov: 1407; Prenosov: 108
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