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Comparison of methods for detection of four common nosocomial pathogens on hospital textiles
Sabina Fijan, Sonja Šostar-Turk, Urška Rozman, 2014, izvirni znanstveni članek

Opis: Introduction: Although the most common vehicle for transmission of health-care acquired infections is the personto- person transmission route, the role of environment should not be ignored and hospital linen may contribute to the spreading of nosocomial infections. The contact plate method and swabbing are common methods for sampling microorganisms on textiles; however, results are available after two days as they are based on incubation followed by phenotypeidentification. An important alternative is using quick wash-off methods followed by PCR detection, which shortens the identification process from two days to a few hours. Methods: The following test microorganisms at different concentrations were inoculated onto textile swatches and dried overnight: Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and Clostridium difficile. RODAC plate sampling as well as a non-destructive wash-off method for capturing microorganisms from the textilesusing a Morapex device were used. The elution suspension from the Morapex device was used for two methods. In the first method, classical incubation on selective media followed by phenotypic identification was used and in the second method DNA was extracted from the elution suspension followed by amplification and agarose gel electrophoresis to visualize amplified products. Conclusions: All chosen bacteria were found using all methods. However, the most sensitive proved to be detection using PCR amplification as we detected the sample with initial concentration of 102 cfu/mL inoculated onto the textile surface before drying. The final detectablerecovered bacterial concentration on textiles was up to 10 cfu/mL.
Ključne besede: health care associated infections, hospital textiles, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Clostridium difficile, Morapex
Objavljeno: 05.04.2017; Ogledov: 1024; Prenosov: 306
.pdf Celotno besedilo (811,70 KB)
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Diversity of spa types among MRSA isolates from Maribor University hospital
Božena Kotnik-Kevorkijan, Marija Klasinc, Slavica Lorenčič Robnik, Živa Petrovič, Maja Rupnik, 2009, izvirni znanstveni članek

Opis: Background: Typing of pathogens is an important part in control and preventionof health care-associated infections. For methilcillin-resistant S. aureus (MRSA) a new sequence-based and easily internationally comparable typing method, spa typing, was recently described. We have used this method to analyze the MRSA types present in our hospital and to compare them with types reported elsewhere. Methods: 63 MRSA strains isolated from patients in Maribor University Hospital (MUH) during the year 2006 were spa typed. Typing data were compared to the patient hospitalization data to detect possible spatial and temporal clusters. Results: Sixty-three MRSA strains were distributed into 12 spa types. Seven spa types were represented only by a single isolate. The three most prevalent types (t001, t288, t003) included 80 % of all strains. The most prevalent type, t001, was present in many differentwards and during entire year. This type is also one of the mostly isolated types worldwide. High prevalence of other two types seems to be associated with small scale transmission events. Conclusions: Most of the spa types present in MUH are well known and widespread also elsewhere in Slovenia, in other EU countries and worldwide. Typing has helped us to follow the introduction of different MRSA types to the hospital environment and to detect occasional transmissions.
Ključne besede: MRSA, typing, spa types, health care-associated infections
Objavljeno: 10.07.2015; Ogledov: 814; Prenosov: 71
.pdf Celotno besedilo (156,26 KB)
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