1. Addressing the needs of the rapidly aging society through the development of multifunctional bioactive coatings for orthopedic applicationsTinkara Mastnak, Uroš Maver, Matjaž Finšgar, 2022, pregledni znanstveni članek Opis: The unprecedented aging of the world’s population will boost the need for orthopedic
implants and expose their current limitations to a greater extent due to the medical complexity of
elderly patients and longer indwelling times of the implanted materials. Biocompatible metals with
multifunctional bioactive coatings promise to provide the means for the controlled and tailorable
release of different medications for patient-specific treatment while prolonging the material’s lifespan
and thus improving the surgical outcome. The objective of this work is to provide a review of several
groups of biocompatible materials that might be utilized as constituents for the development of
multifunctional bioactive coatings on metal materials with a focus on antimicrobial, pain-relieving,
and anticoagulant properties. Moreover, the review presents a summary of medications used in
clinical settings, the disadvantages of the commercially available products, and insight into the latest
development strategies. For a more successful translation of such research into clinical practice, extensive knowledge of the chemical interactions between the components and a detailed understanding
of the properties and mechanisms of biological matter are required. Moreover, the cost-efficiency of
the surface treatment should be considered in the development process.
Ključne besede: biomaterials, orthopedic implants, multifunctional coatings, drug delivery, antimicrobial, pain relief
Objavljeno v DKUM: 09.04.2025; Ogledov: 0; Prenosov: 1
 Celotno besedilo (21,61 MB)
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2. Review of potential drug-eluting contact lens technologiesTina Lovrec-Krstič, Kristjan Orthaber, Uroš Maver, Tomislav Šarenac, 2023, pregledni znanstveni članek Opis: The field of ophthalmology is expanding exponentially, both in terms of diagnostic and therapeutic capabilities, as well as the worldwide increasing incidence of eye-related diseases. Due to an ageing population and climate change, the number of ophthalmic patients will continue to increase, overwhelming healthcare systems and likely leading to under-treatment of chronic eye diseases. Since drops are the mainstay of therapy, clinicians have long emphasised the unmet need for ocular drug delivery. Alternative methods, i.e., with better compliance, stability and longevity of drug delivery, would be preferred. Several approaches and materials are being studied and used to overcome these drawbacks. We believe that drug-loaded contact lenses are among the most promising and are a real step toward dropless ocular therapy, potentially leading to a transformation in clinical ophthalmic practice. In this review, we outline the current role of contact lenses in ocular drug delivery, focusing on materials, drug binding and preparation, concluding with a look at future developments.
Ključne besede: contact lens materials, advanced ocular drug delivery, dropless ocular therapy, drug-laden contact lens
Objavljeno v DKUM: 03.12.2024; Ogledov: 0; Prenosov: 1
Celotno besedilo (4,80 MB)
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3. PREPARATION OF AMINO ACID AND PEPTIDE POLYSACCHARIDE DERIVATIVES AND THEIR APPLICATION AS BIOMATERIALS : doctoral dissertationAna Bratuša, 2024, doktorska disertacija Opis: The derivatization of the polysaccharide dextran with N-protected amino acids (Boc-L-Phenylalanine, BocGlycine, Boc-L-Cysteine, and Boc-L-Cysteine-S-Trt) and peptides (Boc-L-DiPhenylalanine, Boc DiGlycine, and 2,5-diketopiperazine) as the basis for biomaterial preparation is presented in this Doctoral Dissertation. Such prepared dextran derivatives are intended to mimic the proteoglycan complex (PGs), one of the most important structural and functional biomacromolecules in the extracellular matrix (ECM) of tissue. Nowadays, developments in biomaterials are focusing increasingly on the preparation and use of biomimetic molecular structures to achieve positive results in tissue engineering (TE) and drug delivery. Designing and synthesizing these biomimetic materials, however, requires sophisticated chemical and material preparation methods, knowledge that is, currently, unexplored.
In this work, we developed a suitable procedure for dextran derivatization, and investigated the most optimal reaction or deprotection conditions (temperature and time) and isolation/purification methods. The structures of the obtained BocPhe-Dex, BocGly-Dex, BocCys-Dex, and BocSTLC-Dex were analyzed with FTIR, NMR, SEC-MALS, and EA. The results showed that dextran derivatization was successful in all cases except in the case of dextran derivatization with BocCys. Investigation of the effect of the derivatization conditions and purification on the stability, purity, and other important chemical and physical properties of the obtained product, showed that the temperature and time of derivatization do not have a bigger effect on the products' properties, while the purification method, on the other hand, has. Its effect is visible in the product's purity and mass yields of products prepared under the same reaction conditions.
Derivatization of dextran with peptides (Boc-L-DiPhenylalanine, BocDiGlycine, and 2,5-diketopiperazine) was performed using the CDI coupling agent or Amberlite-IR 120 as a catalyst. The products were analyzed with FTIR and 1H and 13C NMR. The results showed successful dextran derivatization in the case of BocDiPhenylalanine and BocDiGlycine, while, in the case of 2,5-diketopiperazine, a reaction covalent bond with the dextran was not confirmed.
BocPhe-Dex and BocSTLC-Dex were selected as the most optimal amino acid-dextran derivatives for further preparation of 3D formulations in the shape of nanoparticles (NPs). Nanoparticles were prepared with the emulsion/solvent evaporation method from the obtained BocPhe-Dex and BocSTLC-Dex products (prepared in the first stage of this Doctoral Dissertation). SEM analysis showed that the prepared NPs were homogeneous and nicely spherical, with an average dry diameter of 325 ± 118 nm in the case of BocSTLC-Dex, and 1039 ± 382 nm in the case of NPs prepared from BocPhe-Dex. All the prepared NPs retained their proper spherical shape and stability during the acidic treatment, and so confirmed their potential for further functionalization and applications for drug delivery. The BocSTLC-Dex NPs were also evaluated with cell viability tests, which showed that the prepared NPs were not cytotoxic, one of the most important characteristics for the drug delivery applications of NPs.
This work serves as a basis for further studies on the derivatization of polysaccharides with amino acids and peptides, and their application in tissue engineering or drug delivery.
Ključne besede: Amino Acid-Dextran derivatives, Peptide-Dextran derivatives, Proteoglycan complex, 3D formulation, Nanoparticles, Drug delivery
Objavljeno v DKUM: 18.10.2024; Ogledov: 0; Prenosov: 40
Celotno besedilo (9,08 MB) |
4. Multifunctional iron oxide nanoparticles as promising magnetic biomaterials in drug elivery : a ǂreviewKatja Vasić, Željko Knez, Maja Leitgeb, 2024, pregledni znanstveni članek Ključne besede: multifunctional biomaterials, bio-composites, nanocarriers, magnetic nanoparticles, modification, drug delivery
Objavljeno v DKUM: 20.08.2024; Ogledov: 72; Prenosov: 16
Celotno besedilo (5,66 MB) |
5. Functionalisation of silicone by drug-embedded chitosan nanoparticles for potential applications in otorhinolaryngologyUrban Ajdnik, Lidija Fras Zemljič, Matej Bračič, Uroš Maver, Olivija Plohl, Janez Rebol, 2019, izvirni znanstveni članek Ključne besede: silicone, tympanostomy tube, chitosan, nanoparticles, drug delivery, antimicrobial activity
Objavljeno v DKUM: 22.03.2019; Ogledov: 1460; Prenosov: 6 |
6. Antimicrobial medical textiles based on chitosan nanoparticles for gynaecological treatmentTijana Ristić, 2014, doktorska disertacija Opis: The aim of this dissertation was to develop a novel medical tampon for alternative gynaecological treatment using chitosan nanoparticles as an antimicrobial agent or as a drug delivery system. For this purpose viscose tampon band was used and functionalized with chitosan and trimethyl chitosan nanoparticles. A comprehensive of interactions between chitosan and cellulose as well as characterization of prepared materials were done. At the beginning, chitosan (CS) and trimethyl chitosan (TMC) solutions, as well as nanoparticles synthesised by ionic gelation were studied. Their characterization was focused on determining the charge and antimicrobial properties against common pathogenic microorganism. The influence of cationic charge on the inhibition of microbial growth was confirmed. Since CS and TMC solutions and nanoparticles dispersions exhibited antibacterial activity against Lactobacillus, a detailed investigation in chitosan’s antimicrobial mode of action was performed using a novel diffusion nuclear magnetic resonance (D-NMR). D-NMR allowed the monitoring of intra- and extracellular water exchange from the cells indicating the membrane alteration and leakage of intracellular constituencies. Further, in order to study the adsorption phenomena and molecular interactions between CS/TMC (solution or nanoparticles) and cellulose material, model cellulose surfaces were used, and adsorption was studied by quartz crystal microbalance with dissipation. CS and TMC were favourably deposited onto cellulose model surface at higher ionic strength, higher pH values, i.e. factors causing lower solubility, where the presence of electrostatic interactions was negligible and non-electrostatic interactions were dominant. The knowledge gained from the model surfaces was extremely helpful in characterization of real systems, i.e. functionalized cellulose fibres and for understanding the obtained results. Immobilization of CS and TMC (in the form of solution and/or nanoparticles) onto cellulose viscose fibres was confirmed with several analytical methods. The attachment of chitosan onto fibres was reversible, as endorsed with desorption studies mimicking the conditions of vaginal usage. Evaluation of antimicrobial properties was performed using two different techniques, both revealing a high inhibition of the tested microorganism. In addition, Lactobacillus susceptibility testing has shown that chitosan-coated fibres do not have any negative influence on the resident microbiota. Assessment of in-vitro cytotoxicity demonstrated that samples do not cause a cytotoxic effect in direct contact. Additionally, model drug was incorporated into chitosan nanoparticles and subsequently attached onto fibres in order to create modern, vaginal drug delivery systems. Antimicrobial medical textiles investigated in the scope of this dissertation show the potential for their exploitation in gynaecological field as preventive or curative treatment without triggering any adverse effects for the user.
Ključne besede: chitosan, N, N, N-trimethyl chitosan, nanoparticles, antimicrobial activity, cellulose model films, regenerated cellulose fibres, diffusion nuclear magnetic resonance, quartz crystal microbalance, vaginal infections, drug delivery systems, cytotoxicity
Objavljeno v DKUM: 13.02.2014; Ogledov: 3877; Prenosov: 197
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