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1.
Ensiling, in vitro rumen digestion and soaking in slurry altered the germination capacity of Rumex obtusifolius seeds
Anastazija Gselman, Maksimiljan Brus, 2023, izvirni znanstveni članek

Opis: This study investigated whether the process of ensiling and in vitro digestion in rumen juice, as well as the response to soaking in pig or cattle slurry, affects the germination rate and germination energy of Rumex obtusifolius (broad-leaved dock) seeds. Seeds were subjected to different treatments (200 seeds each) in three experiments: (I) seed ensiling (8 weeks) followed by in vitro rumen digestion (24, 36 and 48 h); (II) the soaking of non-ensiled and ensiled seeds in cattle or pig slurry (2, 4 and 24 weeks); and (III) the in vitro rumen digestion (24, 36 and 48 h) of non-ensiled and ensiled seeds followed by soaking in cattle or pig slurry (24 weeks). The control treatment included untreated seed (0—non-ensiled seed; 0—no in vitro rumen digestion; and 0—no soaking in slurry). Germination tests (germination rate and germination energy) were then conducted in four replicates in the germination chamber under alternating day (20–35 °C for 14 h under light) and night conditions (17–20 °C for 10 h without light) at 75% relative humidity. Experiment I showed that ensiling significantly (p ≤ 0.001) reduced both the germination rate and germination energy of R. obtusifolius seeds. In addition, the length of in vitro digestion duration that the non-ensiled seeds were subjected to significantly (p ≤ 0.001) reduced their germination energy but not the total germination rate. However, the seeds that were subjected to the process of ensiling and in vitro digestion in the rumen lost their germination completely. The Experiment II investigated the effects of soaking non-ensiled seeds in slurry and showed that germination rates were comparable in pig and cattle slurry. Longer soaking times significantly reduced the germination rate, with no germination observed after 24 weeks. The Experiment III considered the combined effects of in vitro digestion and slurry soaking and showed that rumen digestion reduced the proportion of germinable seeds. Germination was inhibited in pig slurry, while in cattle slurry, a decreasing germination rate was observed with increasing digestion time.
Ključne besede: broad-leaved dock, in vitro rumen digestion, ensiling, pig and cattle slurry, seed germination
Objavljeno v DKUM: 05.04.2024; Ogledov: 229; Prenosov: 6
.pdf Celotno besedilo (293,08 KB)
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2.
Improvement of biogas production utilizing a complex anaerobic digestion model and gradient-based optimization
Tina Kegl, Breda Kegl, Marko Kegl, 2024, izvirni znanstveni članek

Opis: : Anaerobic digestion (AD) is a promising technology for renewable energy production from organic waste. In order to maximize the produced biogas quantity and quality, this paper deals with the optimization of the AD process in a CSTR bioreactor of a full-scale biogas plant. For this purpose, a novel approach was adopted coupling, a highly complex BioModel for AD simulation, and a gradient-based optimization method. In order to improve AD performance, the dosages of various types of biological additives, the dosages of inorganic additives, and the temperature in the bioreactor were optimized in three different scenarios. The best biogas quality was obtained using multi-objective optimization, where the objective function involves the following two conflicting objectives: the maximization of biogas production and minimization of the needed heating energy. The obtained results show that, potentially, the content of CH4 can be increased by 11%, while the contents of H2, H2S, and NH3 can be reduced by 30%, 20%, and 81% when comparing the simulation results with the experimental data. The obtained results confirm the usefulness of the proposed approach, which can easily be adapted or upgraded for other bioreactor types.
Ključne besede: additives, anaerobic digestion, approximation method, BioModel, complex substrate, gradient-based optimization, process conditions
Objavljeno v DKUM: 12.03.2024; Ogledov: 218; Prenosov: 15
.pdf Celotno besedilo (7,33 MB)
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3.
Optimizacija priprave proteinov krvne plazme z razgradnjo na peptide v raztopini za proteomsko analizo z masno spektrometrijo : diplomsko delo univerzitetnega študijskega programa I. stopnje
Eva Zajšek, 2023, diplomsko delo

Opis: V diplomskem delu sta predstavljena danes najpogostejša načina priprave vzorcev proteinov z razgradnjo na peptide za proteomsko analizo z masnim spektrometrom: SDS PAGE in stopenjska priprava v raztopini, ki vključuje denaturacijo, redukcijo, alkilacijo proteinov in njihovo cepitev na peptide s tripsinom. Naš namen je bil skrajšati, poenostaviti in poceniti v znanstveni literaturi opisani protokol stopenjske priprave vzorcev proteinov v raztopini, vendar kljub temu ohraniti dobro razgradnjo proteinov in identifikacijo z masnim spektrometrom. V delu je tako podrobneje prikazana analiza optimizacije priprave vzorcev v raztopini: različni reagenti, različne koncentracije, združevanje korakov, dodajanje korakov, različne temperature in različno dolge inkubacije. Rezultati kažejo, da protokola ne moremo skrajšati na račun časa inkubacije tripsina, vendar pa lahko to storimo z združitvijo korakov denaturacije, redukcije in alkilacije ter skrajšanim časom inkubacije teh korakov. Kot boljši alkilacijski reagent se je v primerjavi z jodoacetamidom (IAM) zaradi manjšega števila neželenih modifikacij izkazal kloroacetamid (CAM), za oba reagenta pa je primerna koncentracija pod 10 mM. Ugotovili smo, da dodaten korak prekinitve alkilacije z ditiotreitolom (DTT) zaradi prihranka časa in kemikalij ni primeren, saj ne zmanjša števila neželenih modifikacij. Povzeti protokol smo nekoliko pocenili z zamenjavo alkilacijskih reagentov, saj je kloroacetamid (CAM) cenejši in ga je na voljo za več eksperimentov, sicer pa ceno celotnega eksperimenta določa predvsem poraba tripsina, čigar cena znaša okrog 2,23 EUR za 1 μL.
Ključne besede: proteomika, SDS PAGE, in-solution-digestion, reagenti, masna spektroskopija, priprava vzorcev
Objavljeno v DKUM: 31.03.2023; Ogledov: 564; Prenosov: 43
.pdf Celotno besedilo (4,45 MB)

4.
Building of mini digester for mesophilic anaerobic digestion
Bogomir Muršec, Peter Vindiš, 2009, strokovni članek

Opis: Anaerobic digestion is the biological degradation of organic material in the absence of air andmayimply environmental benefits with regard to waste treatment, pollution reduction, energy production, and improvements in agricultural practices. Ananaerobic digester is a system that harnesses this natural process to treat waste, produce biogas that can be converted to heat and electricity and anaerobic digestate, a soil improving material. The paper presents the building of a mini digester for mesophilic anaerobic digestion.With the mini digester the amount of biogas production (methane) from different organic wastes is observed. The basic structure is made of stainless steel, on which other components are fixed. The most important components are the eudiometers, pump and heater with thermostat, fermenters, thermometer and barometer. The experiment is made according to DIN 38 414, part 8. After building of a mini digester some experiments with different organic materials are made.
Ključne besede: mini digester, mesophilic anaerobic digestion, organic wastes
Objavljeno v DKUM: 11.07.2017; Ogledov: 1691; Prenosov: 106
.pdf Celotno besedilo (493,38 KB)
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5.
Biogas as a renewable energy source
Matjaž Ošlaj, Bogomir Muršec, 2010, pregledni znanstveni članek

Opis: Renewable energy technology does not simply produce energy, heat and transport fuel, but also offers the opportunity to live in the footsteps of a reasonable future development. In Europe and other industrialized regions, the main reason for the development of renewable energy is the environment, in particular the concern in relation to global climate change and the need to improve security and diversity of energy supply. In the developing countries, they promise a new hope for renewable primary energy supply in regions without conventional energy and provide an opportunity for sustainable development. Productionof "green energy" from biogas, which is among the renewable energy sources, promises an environmentally less damaging way of obtaining energy by reducing CO emissions into the environment and reduces energy dependence on imported energy sources. Biogas production is of major importance for the sustainable use of agrarian biomass as renewable energy source.
Ključne besede: anaerobic digestion, biogas, manure, renewable energy
Objavljeno v DKUM: 11.07.2017; Ogledov: 1650; Prenosov: 104
.pdf Celotno besedilo (194,11 KB)
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6.
In vitro selection and characterization of new probiotic candidates from table olive microbiota
Cristian Botta, Tomaž Langerholc, Avrelija Cencič, Luca Cocolin, 2014, izvirni znanstveni članek

Opis: To date, only a few studies have investigated the complex microbiota of table olives in order to identify new probiotic microorganisms, even though this food matrix has been shown to be a suitable source of beneficial lactic acid bacteria (LAB). Two hundred and thirty eight LAB, belonging to Lactobacillus plantarum, Lactobacillus pentosus and Leuconostoc mesenteroides species, and isolated from Nocellara Etnea table olives, have been screened in this survey through an in vitro approach. A simulation of transit tolerance in the upper human gastrointestinal tract, together with autoaggregation and hydrophobicity, have been decisive in reducing the number of LAB to 17 promising probiotics. None of the selected strains showed intrinsic resistances towards a broad spectrum of antibiotics and were therefore accurately characterized on an undifferentiated and 3D functional model of the human intestinal tract made up of H4-1 epithelial cells. As far as the potential colonization of the intestinal tract is concerned, a high adhesion ratio was observed for Lb. plantarum O2T60C (over 9%) when tested in the 3D functional model, which closely mimics real intestinal conditions. The stimulation properties towards the epithelial barrier integrity and the in vitro inhibition of L. monocytogenes adhesion and invasion have also been assessed. Lb. plantarum S1T10A and S11T3E enhanced trans-epithelial electrical resistance (TEER) and therefore the integrity of the polarized epithelium in the 3D model. Moreover, S11T3E showed the ability to inhibit L. monocytogenes invasion in the undifferentiated epithelial model. The reduction in L. monocytogenes infection, together with the potential enhancement of barrier integrity and an adhesion ratio that was above the average in the 3D functional model (6.9%) would seem to suggest the Lb. plantarum S11T3E strain as the most interesting candidate for possible in vivo animal and human trials.
Ključne besede: antibiotics, bacteria pathogen, cell metabolism, olives, probiotics, digestion
Objavljeno v DKUM: 19.06.2017; Ogledov: 1459; Prenosov: 347
.pdf Celotno besedilo (1,63 MB)
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Aerobic digester design for the biodegradation of plant tannins in industrial wastewater
Marko Tramšek, Andreja Goršek, 2008, izvirni znanstveni članek

Opis: This paper describes aerobic digester design for the biodegradation of plant tannins in industrial wastewater. For optimal design, using the criterion of minimal total holding time, some experimental investigations into tannins' biodegradation rate in industrial wastewater were performed in the first part of this research. The chemical oxygen demand method (COD) was applied to follow the tannins degradation rate. The kinetic parameters of a supposed Aiba's inhibition kinetic model were determined using experimental data. In the second part of the study, equations for determining the optimal volumes of two in series connected aerobic digesters were established. Furthermore, a comparison is presented of volumes between one and two in series connected aerobic digester systems regarding wastewater volume flow rate, qV = 120 m3 d-1 and wastewater treatment efficiency, COD = 9 8%.
Ključne besede: digester design, plant tannins, Aiba's inhibition kinetic model, aerobic digestion, industrial wastewater
Objavljeno v DKUM: 31.05.2012; Ogledov: 2060; Prenosov: 61
URL Povezava na celotno besedilo

10.
The aerobic biodegradation kinetics of plant tannins in industrial wastewater
Marko Tramšek, Andreja Goršek, Peter Glavič, 2006, izvirni znanstveni članek

Opis: This paper describes an experimental determination of the biodegradation rate for tannins present in industrial wastewater, after the extraction of chestnut chips. Experiments were performed in a laboratory aerobic reactor (Armfield) by using biomass from an existing industrial wastewater treatment plant. The outlet tannins concentration was determined under various processing conditions. Simultaneously, an optical microscope was used to monitor the mix of microbiological cultures in the biomass. On the basis of data obtained in experiments, non-linear regression was used to perform parametric analysis of various kinetic models, which took into account inhibition, as quoted in literature (Haldane, Edwards, Aiba, Luong). The statistical analysis, based on the P-criterion, F-criterion, adjusted coefficient of determination, Kolmogorov-Smirnov test and root mean squared error, showed that the biodegradation of plant tannins in industrial wastewater under selected conditions for aerobic digestion, can be most successfully described statistically by the Aiba's kinetic model.
Ključne besede: plant tannins, susbtrate inhibition, kinetic models, statistic analysis, aerobic industrial wastewater digestion
Objavljeno v DKUM: 31.05.2012; Ogledov: 1919; Prenosov: 49
URL Povezava na celotno besedilo

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