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Opis: Antimicrobial resistance (AMR) is increasing worldwide. This is due to the widespread and often uncontrolled release of antibiotics into surface water, drinking water, and the food chain. The traces of antibiotics (ng/L to μg/L) bioaccumulate, disrupt ecosystems, and accelerate AMR, yet regulatory monitoring remains inadequate. Sensitive analytical methods for the detection and quantification of antibiotics at trace levels in complex matrices are therefore essential. Conventional techniques, i.e., liquid or gas chromatography, mass spectrometry, and capillary electrophoresis, offer high accuracy but are associated with costly instrumentation, lengthy workflows, and extensive sample preparation. Electrochemical sensors based on advanced nanomaterials, particularly magnetic nanoparticles (MNPs), have attracted considerable interest due to their advantages in sensitivity and selectivity, wide linear dynamic ranges, extremely low limits of detection (LOD) and quantification (LOQ), low instrument cost, and rapid response. This review provides a critical overview of recent advances in MNP-based electrochemical platforms for antibiotic detection. The focus is on wet-chemical synthesis routes, modification approaches, and strategies for integrating magnetic nanocomposites into electrodes. Synergistic improvements through hybrid architectures are emphasized, combining MNPs with carbon nanomaterials, biopolymers, metal–organic frameworks, and molecularly imprinted polymers. Trends over the last five years have shown that applications in environmental monitoring, food safety, and drinking water are increasing. We summarize the key performance metrics, outline the current technical bottlenecks, such as long-term stability and large-scale manufacturing, and provide an outlook for the future. Taken together, these developments position MNP-based electrochemical sensors as versatile, highly effective tools for curbing antibiotic pollution and slowing the advancement of AMR.
Ključne besede: electrochemical sensor, antibiotics̀ determination, magnetic nanomaterials, material chemistry, nanocomposites, antimicrobial resistance, food safety, environmental sensing
Objavljeno v DKUM: 04.12.2025; Ogledov: 0; Prenosov: 2
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Opis: Amoxicillin (AMX) and Ciprofloxacin (CIP) are antibiotics commonly used in human medicine with high environmental toxicity and poor biodegradability. They have been found in various hospital effluents and groundwater, and their environmental impact is still not fully understood. In this work, we investigated the possibility of treating model wastewaters containing the antibiotics AMX and CIP using ozonation, with the addition of H$_2$O$_2$ under various conditions, including different pH values, H$_2$O$_2$, and ozone dosages. The quantification of and treatment efficacy for antibiotic removal were determined via solid phase extraction followed by chromatographic separation by liquid chromatography coupled with tandem triple quadrupole mass spectrometry (LC/MS/MS). This analytical system is quite efficient for the detection of all major antibiotic classes, even if they are present at very low concentrations. The efficiency of ozonation was determined by measuring the TOC (Total Organic Carbon) changes after ozonation of the model wastewater and by measuring the concentration of the two antibiotics. In a sequential activated sludge process of ozone-treated model wastewater, almost complete TOC removal and an overwhelming decrease in antibiotic concentrations (up to 99%) were observed. Ozonation resulted in complete removal of AMX and CIP in less than 30 and 120 min, respectively. The results of this work indicate that ozonation could be a suitable pretreatment method to reduce the toxicity of contaminants (AMX and CIP) and improve the biodegradability of hospital wastewater.
Ključne besede: antibiotics, amoxicillin, AMX, ciprofloxacin, CIP, hospital wastewater, hydrogen peroxide, ozone, sludge, water treatment
Objavljeno v DKUM: 06.08.2024; Ogledov: 75; Prenosov: 16
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Opis: To date, only a few studies have investigated the complex microbiota of table olives in order to identify new probiotic microorganisms, even though this food matrix has been shown to be a suitable source of beneficial lactic acid bacteria (LAB). Two hundred and thirty eight LAB, belonging to Lactobacillus plantarum, Lactobacillus pentosus and Leuconostoc mesenteroides species, and isolated from Nocellara Etnea table olives, have been screened in this survey through an in vitro approach. A simulation of transit tolerance in the upper human gastrointestinal tract, together with autoaggregation and hydrophobicity, have been decisive in reducing the number of LAB to 17 promising probiotics. None of the selected strains showed intrinsic resistances towards a broad spectrum of antibiotics and were therefore accurately characterized on an undifferentiated and 3D functional model of the human intestinal tract made up of H4-1 epithelial cells. As far as the potential colonization of the intestinal tract is concerned, a high adhesion ratio was observed for Lb. plantarum O2T60C (over 9%) when tested in the 3D functional model, which closely mimics real intestinal conditions. The stimulation properties towards the epithelial barrier integrity and the in vitro inhibition of L. monocytogenes adhesion and invasion have also been assessed. Lb. plantarum S1T10A and S11T3E enhanced trans-epithelial electrical resistance (TEER) and therefore the integrity of the polarized epithelium in the 3D model. Moreover, S11T3E showed the ability to inhibit L. monocytogenes invasion in the undifferentiated epithelial model. The reduction in L. monocytogenes infection, together with the potential enhancement of barrier integrity and an adhesion ratio that was above the average in the 3D functional model (6.9%) would seem to suggest the Lb. plantarum S11T3E strain as the most interesting candidate for possible in vivo animal and human trials.
Ključne besede: antibiotics, bacteria pathogen, cell metabolism, olives, probiotics, digestion
Objavljeno v DKUM: 19.06.2017; Ogledov: 1573; Prenosov: 374
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