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1.
Preparation of enantiomers using high pressure technologies
Paul Thorey, 2010, doktorska disertacija

Opis: The study of two different methods of obtaining chiral alcohols is proposed herein. The requirement of the relatively new paradigm of green chemistry associated with clean technologies such as biocatalysis or non-conventional solvents, dense gases, was focused at. Indeed, the two methods of production of chiral alcohols were: - the conversion of acetophenone into (R)-1-phenylethanol in dense gases catalysed by Lactobacillus brevis alcohol dehydrogenase and its coenzyme, NADP/H; - the resolution of (±)-trans-1,2-cyclohexanediol by cocrystal formation with tartaric acid followed by supercritical extraction. In both cases high enantiopurities were achieved (ee>99%).
Ključne besede: High-pressure technologies, enantiomers, green chemistry, R-1-phenylethanol, Lactobacillus brevis, alcohol dehydrogenase, NADP, liquid propane, enzyme deactivation, resolution, trans-1, 2-cyclohexanediol, tartaric acid, cocrystal, supercritical carbon dioxide, extraction, X-ray diffraction, differential scanning calorimetry
Objavljeno: 02.02.2011; Ogledov: 1757; Prenosov: 53
.pdf Polno besedilo (6,25 MB)

2.
PRIPRAVA PODATKOVNE BAZE PROBIOTIČNIH IN PROTEKTURNIH KULTUR
Vanja Žiher, 2012, magistrsko delo

Opis: Probiotične bakterije se med seboj razlikujejo. Ene so uspešnejše pri borbi proti škodljivim bakterijam, druge pri spodbujanju delovanja prebavil, tretje zopet pri spodbujanju imunskega sistema. Vse boljše poznavanje mehanizmov njihovega delovanja in vse več kliničnih raziskav, opravljenih v zadnjih letih, nakazuje možnost uspešne uporabe probiotičnih bakterij tako v preventivi, kot tudi podpori pri zdravljenju različnih bolezni. V magistrski nalogi, smo uporabili anketni vprašalnik in na podlagi pridobljenih rezultatov, smo opisovali lastnosti in klinično uporabo določenih probiotičnih sevov. Zbrani podatki, bodo predstavljeni na spletni strani Fakultete za Kmetijstvo in biosistemske vede Maribor.
Ključne besede: probiotiki, imunski sistem, Lactobacillus, Bifidobacterium, Enterococcus
Objavljeno: 25.05.2012; Ogledov: 1272; Prenosov: 181
.pdf Polno besedilo (1,97 MB)

3.
A co-culture model of the developing small intestine offers new insight in the early immunomodulation of enterocytes and macrophages by Lactobacillus spp. through STAT1 and NF-kB p65 translocation
Martin Trapečar, Aleš Goropevšek, Mario Gorenjak, Lidija Gradišnik, Marjan Rupnik, 2014, izvirni znanstveni članek

Opis: The early establishment of a complete microbiome has been shown to play an integral part in the development and maintenance of an intact intestine and its immune system, although much remains unknown about the specific mechanisms of immune modulation in newborns. In our study we show in a co-culture model of the undeveloped small intestine that members of Lactobacillus spp. influence STAT1 and NF-kB p65 nuclear translocation in both intestinal epithelial cells as well as underlying macrophages. Moreover, by using imaging flow cytometry we were able to monitor each individual cell and create a framework of the percentage of cells in which translocation occurred in challenged versus control cell populations. We also observed a significant difference in baseline translocation in intestinal cells when cultured alone versus those in a co-culture model, underpinning the importance of 3D models over monolayer set-ups in epithelial in vitro research. In conclusion, our work offers new insights into the potential routes by which the commensal microbiome primes the early immune system to fight pathogens, and shows how strain-specific these mechanisms really are.
Ključne besede: microbiome, Lactobacillus, immune system, pathogens
Objavljeno: 19.06.2017; Ogledov: 51; Prenosov: 0
.pdf Polno besedilo (2,86 MB)

4.
Microorganisms with claimed probiotic properties: an overview of recent literature
Sabina Fijan, 2014, pregledni znanstveni članek

Opis: Probiotics are defined as live microorganisms, which when administered in adequate amounts, confer a health benefit on the host. Health benefits have mainly been demonstrated for specific probiotic strains of the following genera: Lactobacillus, Bifidobacterium, Saccharomyces, Enterococcus, Streptococcus, Pediococcus, Leuconostoc, Bacillus, Escherichia coli. The human microbiota is getting a lot of attention today and research has already demonstrated that alteration of this microbiota may have far-reaching consequences. One of the possible routes for correcting dysbiosis is by consuming probiotics. The credibility of specific health claims of probiotics and their safety must be established through science-based clinical studies. This overview summarizes the most commonly used probiotic microorganisms and their demonstrated health claims. As probiotic properties have been shown to be strain specific, accurate identification of particular strains is also very important. On the other hand, it is also demonstrated that the use of various probiotics for immunocompromised patients or patients with a leaky gut has also yielded infections, sepsis, fungemia, bacteraemia. Although the vast majority of probiotics that are used today are generally regarded as safe and beneficial for healthy individuals, caution in selecting and monitoring of probiotics for patients is needed and complete consideration of risk-benefit ratio before prescribing is recommended.
Ključne besede: Lactic-acid bacteria, Lactobacillus, Bifidobacterium, Saccharomyces, Enterococcus, Streptococcus, Pediococcus, Leuconostoc, Bacillus, Escherichia coli
Objavljeno: 21.06.2017; Ogledov: 71; Prenosov: 3
.pdf Polno besedilo (267,98 KB)

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